| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 1,310,041 | (C)5→6 | coding (406/1989 nt) | aaeB_2 → | p‑hydroxybenzoic acid efflux pump subunit AaeB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,310,036 | 1 | . | C | 97.1% | 259.8 / ‑0.4 | 68 | coding (401/1989 nt) | aaeB_2 | p‑hydroxybenzoic acid efflux pump subunit AaeB |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (40/26); total (41/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑A‑CCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGC > ACB143/1309902‑1310162 | ccTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGacc‑a > 7:119496/1‑135 (MQ=255) tCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑A‑cccccc < 5:251578/135‑2 (MQ=255) tCTGCTTACCTTGGCGCGTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑A‑cccccc < 5:49172/135‑2 (MQ=255) tACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTTCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑A‑cccccctgaaa > 8:360151/1‑128 (MQ=255) ccTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGt < 4:129130/134‑1 (MQ=255) ccTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGt < 4:123168/134‑1 (MQ=255) ttGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTAtt > 7:216901/1‑135 (MQ=255) ttGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTc > 5:371658/1‑136 (MQ=255) tGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTc > 3:63528/1‑135 (MQ=255) gcgcTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTc < 7:287858/133‑1 (MQ=255) gctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGcc < 2:339667/134‑1 (MQ=255) gctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGcc < 3:52343/134‑1 (MQ=255) ctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGt < 8:104686/135‑1 (MQ=255) ctggcGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑A‑CCCCCTGCAAGTATTCGACCAGGCCGTgg > 6:93684/1‑136 (MQ=255) gtACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGt < 1:26324/135‑1 (MQ=255) aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAcc > 6:146378/1‑136 (MQ=255) aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAcc > 6:93277/1‑136 (MQ=255) aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAc > 3:317845/1‑135 (MQ=255) aCCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAc > 7:270153/1‑135 (MQ=255) aGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAc > 1:275533/1‑130 (MQ=255) aGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAc > 1:270236/1‑130 (MQ=255) aGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCgaga > 6:142107/1‑135 (MQ=255) cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATc > 8:84371/1‑135 (MQ=255) cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt < 6:344672/136‑1 (MQ=255) cGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTAACGAGATc > 8:333972/1‑135 (MQ=255) gTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGAAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCTAGTATTCGACCAGGCCGTGGCTCTCTGTACCGAGATCt < 7:139418/135‑1 (MQ=255) gTCCAACCCTTTGCGCATCGCCCGGGGCTATTCCCTCGTGCCGGCCGGCTAAACCGCATGGATCATCGCCATTCCGGCCATTTCCCAA‑CCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCt < 3:147948/135‑1 (MQ=255) ccACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGct > 6:344926/1‑135 (MQ=255) aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGCCC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTg > 5:142804/1‑136 (MQ=255) aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCt > 3:399920/1‑135 (MQ=255) aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTg < 6:128577/136‑1 (MQ=255) aCCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTg > 5:122399/1‑136 (MQ=255) cAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTggg > 5:59413/1‑135 (MQ=255) aGTTGCGCAGCTCCTGTGCCTATGCCTTCGTGCTGGCCGGCTACAACGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTTCAAGTATTCGACCATGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGt < 4:245909/135‑1 (MQ=255) aGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGt < 1:159773/135‑1 (MQ=255) ttGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTAt < 2:178469/135‑1 (MQ=255) cgccgcGCCTGGGCCTATGCCTGCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTCCCGAGATCTGCCTGGGTAgttt > 2:6124/1‑135 (MQ=255) cAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTAGTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCt > 8:111370/1‑135 (MQ=255) cTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTCCCGAGATCTGCCTGGGTATTTTCTGTGCaa > 7:168010/1‑135 (MQ=255) cTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCat > 5:287402/1‑134 (MQ=255) tGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATCGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAc < 4:302738/135‑1 (MQ=255) gggCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAca > 7:258298/1‑135 (MQ=255) gCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagc < 4:14468/135‑1 (MQ=255) ccTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca < 5:157415/136‑1 (MQ=255) cTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca < 7:64857/135‑1 (MQ=255) cTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAAcagcca < 4:16343/135‑1 (MQ=255) gCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGCCC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCcagcag > 8:233830/1‑135 (MQ=255) gCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCcagcag > 8:233833/1‑135 (MQ=255) gCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt > 5:278928/1‑136 (MQ=255) ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCTTGGGTATTTTCTGTGCAACAGCCAGCAGt < 5:344926/135‑1 (MQ=255) ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt < 6:122399/135‑1 (MQ=255) ccTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt < 5:142107/135‑1 (MQ=255) cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCg < 4:70563/96‑1 (MQ=255) cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCg > 3:70563/1‑96 (MQ=255) cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGt < 4:317845/134‑1 (MQ=255) cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTg > 3:401371/1‑135 (MQ=255) cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTg > 3:405451/1‑135 (MQ=255) cTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTg > 1:84904/1‑135 (MQ=255) tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCtt < 3:384036/134‑1 (MQ=255) tGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCtt < 4:55553/134‑1 (MQ=255) gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGc > 1:362990/1‑135 (MQ=255) gCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGc > 3:278882/1‑135 (MQ=255) ggCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGc > 1:333373/1‑134 (MQ=255) cacCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGc > 2:200017/1‑135 (MQ=255) acCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCg < 8:119496/135‑1 (MQ=255) cAGCGATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGt > 7:177950/1‑134 (MQ=255) gATCATCGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGag > 5:115064/1‑134 (MQ=255) atcGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCaa > 5:81508/1‑136 (MQ=255) atcGCCTTGCCGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCaa > 6:295832/1‑136 (MQ=255) cGGCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTgggcggg > 5:392707/1‑135 (MQ=255) ggCCATTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTgggcggg > 6:86883/1‑134 (MQ=255) aTTGACC‑ACCCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGgc > 7:153365/1‑135 (MQ=255) | CCTGTTTCTGCTTACCTTGGCGCTTTGGCTGGCGCTGTGTACCGCAGCGTCCACCCAGTTGCGCAGCGCCTGGGCCTATGCCTTCGTGCTGGCCGGCTACACCGCAGCGATCATCGCCTTGCCGGCCATTGACC‑A‑CCCCCTGCAAGTATTCGACCAGGCCGTGGCTCGCTGTACCGAGATCTGCCTGGGTATTTTCTGTGCAACAGCCAGCAGTGCCTTGCTCTGGCCCATGCGGGTCGAGCAGCAACTGGGCGGGCAGGC > ACB143/1309902‑1310162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |