Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,383,7690CG41.2% 44.7 / 16.0 34intergenic (+539/‑319)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (2/18);  new base G (14/0);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 8.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTG  >  ACB143/5383652‑5383901
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cAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTGGCTG‑GAATTTGGGCGCTgg                                                                                                                     >  6:394729/1‑135 (MQ=255)
      acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTGTTTTTTGGCGG‑GGATTTTGGGGGTGCggggg                                                                                                                >  1:79083/1‑133 (MQ=255)
                   tGTAGTACGGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAt‑tt                                                                                                 <  5:339122/136‑1 (MQ=255)
                   tGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAt‑t                                                                                                  <  8:148938/135‑1 (MQ=255)
                      agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTaa                                                                                               <  2:237946/135‑1 (MQ=255)
                      agtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTaa                                                                                               <  3:152983/135‑1 (MQ=255)
                       gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTG‑GGATTTGGGGGGGGGGGGGGGCAAAATAGGAA‑TTaaa                                                                                              >  3:93456/1‑134 (MQ=255)
                       gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTaa                                                                                               <  2:136152/134‑1 (MQ=255)
                       gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGCCTCCACCAAATCCAAAGAAGAAGACGCCCACGGCCGGCGGTTTTTGTGGCCG‑GGATTAGGTGGCGACGGGTCGCAAAATCGGAT‑TTaa                                                                                               >  8:231749/1‑134 (MQ=255)
                        taCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAAcc                                                                                             <  1:109834/135‑1 (MQ=255)
                        taCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGGCGAAAAATCGGAT‑TTAAcc                                                                                             <  4:69562/135‑1 (MQ=255)
                             aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTTGGCTGTGGATTTTGGTGGGGCGGGGGGAAAAAAAGCTT‑TTAAcccagt                                                                                         >  1:210381/1‑132 (MQ=255)
                              cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTTGGGG‑GAATTTTGGCGCGGCGGGTGGCAAAAACGGGT‑TTTACCCCgttgt                                                                                      >  5:153632/1‑134 (MQ=255)
                              cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTGTTTTTTGGGGG‑GGATTTTTGGGCGGGGGGGGGAAAAATCGGGTGTTTACCCCtta                                                                                        >  3:370189/1‑134 (MQ=255)
                                gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTG‑GTATTTGGGGGGGGCGGGGGGGAAAATCGGAT‑TTTAACCCgttggt                                                                                     >  8:54217/1‑132 (MQ=255)
                                gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCtt                                                                                     <  1:201968/135‑1 (MQ=255)
                                  ggAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTGCGTG‑GAATTTTTGGGCTGCGGGTGGGAAAATCGGAT‑TTAACCCCGTTGTTGTa                                                                                  >  6:64756/1‑136 (MQ=255)
                                   gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTG‑GAATTTGGGGGGGGGGGGGGGGAAAAAAGGAT‑TTAAACCCGTTATTGttg                                                                                 >  6:266737/1‑134 (MQ=255)
                                    aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTGGCTG‑GGATTTTGGGGCTGCGGGGGGAAAAATAGGGG‑TTAAACCCCTTGGTGTAgg                                                                                >  6:95021/1‑136 (MQ=255)
                                            cGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGtt                                                                           <  4:157529/133‑1 (MQ=255)
                                                  ggggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCaaat                                                                   <  8:1609/135‑2 (MQ=255)
                                                                   ggCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTTGGGGG‑GGATTTTGGGGGGGGGGGGGGCAAAAAAGGAT‑TTTAAACCTTTGTTTTTGGGGGGGTTACAAAGATACTGCCTGCGccggg                                                  >  4:273238/1‑132 (MQ=255)
                                                                   ggCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTTGGTG‑GTATTTTGGCGCTGCGGGGCGAAAAATCGGGG‑TTTACCCCGTTGTTGTATGGGGGTTGGCAAAGATTCTGCCGGCGCCGGt                                                  >  7:79965/1‑135 (MQ=255)
                                                                             aaTCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCGG‑GTATTTTGGGGCGGGGGGGGGGAAAAAAGGAT‑TTTAACCCGTTTTTTTTTGGCGTTTGCCAAATATACTGCCTGCGCCGGTTTTGCGtttc                                        >  8:207077/1‑133 (MQ=255)
                                                                               tacAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                      <  2:187878/133‑1 (MQ=255)
                                                                                 cAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGGTG‑GGATTTTGGGGGTGGGGGTGGTAAAATAGGAT‑TTTAAACCATTCTTTTTTGGGGGTTTCCAAAGAAAATTCCTGCGCCGTTTTTAAGTTTCGGtg                                    >  8:216435/1‑134 (MQ=255)
                                                                                      aagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                              <  6:371482/136‑1 (MQ=255)
                                                                                       ataagaCTCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                              <  7:63955/133‑1 (MQ=255)
                                                                                       agaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGTGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                              <  2:306128/135‑1 (MQ=255)
                                                                                       agaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                              <  7:232025/135‑1 (MQ=255)
                                                                                                ccTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGTGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtttt                    <  6:269406/136‑1 (MQ=255)
                                                                                                              ttttGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTggg        <  8:247429/134‑1 (MQ=255)
                                                                                                              ttttGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTggg        <  4:29916/134‑1 (MQ=255)
                                                                                                                   tgCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTg  >  2:243745/1‑135 (MQ=255)
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CAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTG‑GAATTTGGGCGCTGCGGGTCGAAAAATCGGAT‑TTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTG  >  ACB143/5383652‑5383901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑