| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 6,043,196 | 0 | C | A | 34.2% | 80.0 / 35.6 | 73 | A37S (GCC→TCC) | puuD_3 | Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD |
| Reads supporting (aligned to +/- strand): ref base C (25/23); new base A (0/25); total (25/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.43e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB143/6043069‑6043327 | gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gccg > 8:162961/1‑135 (MQ=255)gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑gccg > 2:20632/1‑135 (MQ=255) gTAATGCTGCGGCTCGATATTTGAAGCCGCGCGGGTAAAAACAAGGCCGTGCAGGCTCGCCAGCAGCGGTTGGGGGGGGCTGCCGCCATCACGGGCGGGCAACAGCAGTGGCAAGCGGGAAAA‑GCCGGCCGCCt < 2:307414/134‑1 (MQ=255) tgaggCGGCCCGTTTTTTGAGGGCGAGCGGTTAAAAACAATCCGGGGCTGGCGCGCCAGCCGGGCTGGGGTGGCGCGGCCGCCATCCGGGGCGGGAAAGATCTGTGCCACCCCGGCAAA‑GCCGGCCGCCTCGACa < 8:165353/135‑1 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACtt > 3:399359/1‑135 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCg > 5:402743/1‑136 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGcc > 5:179432/1‑136 (MQ=255) ttGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCg > 2:155332/1‑135 (MQ=255) ggCGTGCCGTAAAAAACATTGCGGGGCTGGCTAGCCTGCAGCGCTTGCGTGGCGCTGCCGCCATCCGGGGCGGGCAAGATCTGTGGCAAGCCGGAAAC‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc < 4:301742/135‑1 (MQ=255) aGCCGTAAAAAACAATGCCGTCCCGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCAACACGGGCCGGCAAGATCCGTGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgagga < 5:183137/136‑1 (MQ=255) ggTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTATCGCCGACCGTGTGCGAGGAGtt > 3:260380/1‑135 (MQ=255) gTAAAACCAATGCGGTGCCGGCGTGCAAGCAGCGCTTGGGGGTCGCTGCCGCCATCACGGGCCGGAAAGATCTGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttct < 6:327772/136‑1 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgc > 1:37285/1‑134 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTATTCCCTACctgc > 1:394554/1‑134 (MQ=255) cGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGACGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgc > 1:114150/1‑134 (MQ=255) tggCTAGCCAGCAGCGCTTGGGTGTCGCTGCCGCCATCACGGGCCGGCATGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 2:112427/133‑1 (MQ=255) aGGCGAGCCAGCTGCGCTTGCGTGTCGGTGCCGCAATCACGGGCGGGAAAGATCAGTCTCAAGCGGTCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 7:219725/134‑1 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTCCCTGCTGACgg > 3:202882/1‑135 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg > 5:1354/1‑135 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg > 5:368799/1‑135 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg > 5:6369/1‑135 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACgg > 8:73778/1‑135 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg > 7:3500/1‑134 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg > 7:230439/1‑134 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg > 1:78595/1‑134 (MQ=255) agcagcGGTTGCGGGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCATGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTg < 4:276766/135‑1 (MQ=255) agcagcGCTTGGGTGGCGCTGCCGCCTGCAGGGGCCGGCAAGATCAGGGCCATGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGa < 6:157033/136‑1 (MQ=255) agcagcGCTTGCGTGTCGCTGCCGCAATCAGGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTg < 7:266303/135‑1 (MQ=255) gCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTATTCCCTACCTGCTGACGGCAGGCGCTGCCACCGATc > 3:222673/1‑134 (MQ=255) gtCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 7:218209/135‑1 (MQ=255) cTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGAcc > 8:153462/1‑135 (MQ=255) cgccATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTATCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt < 4:260380/135‑1 (MQ=255) tccggggcgggcAAGTTCTGTGGCAAGCCGGAAAT‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:297121/132‑1 (MQ=255) tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:402743/136‑1 (MQ=255) tCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:205668/136‑1 (MQ=255) tCACGGGCCGCCAATATCAGGGCAAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:140651/136‑1 (MQ=255) ccccGGGCCGCAAAGATCGGTGCCACG‑CGGCAAACGCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:243330/133‑1 (MQ=255) ccggggCCGTCATGAACAGTGCCAACCGGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:34900/132‑1 (MQ=255) ccggggCCGGAAGTATCTGTGGCACGCGGCAAAA‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:206681/132‑1 (MQ=255) ccggggCCGGAAATATCAGTGGCACGCGGCAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:342556/132‑1 (MQ=255) cccGGGCCGGCAAGAGCAGTGGCAAGCCGGCAAA‑GCCGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:355134/133‑1 (MQ=255) cccGGGCCGCAAATATCCGGGGCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:190258/133‑1 (MQ=255) caggggCCGGAAATATCTGTGGCAAGCGGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:318785/132‑1 (MQ=255) caggggCCGGAAAGATACGGGCCACGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:240867/132‑1 (MQ=255) cACGGGCGGGCAATATCAGTGGCAAGCGGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:35151/135‑1 (MQ=255) cACGGGCCGGCAATATCTGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:164797/135‑1 (MQ=255) cACGGGCCGGCAAGATCTGTGGCAAGCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:114169/135‑1 (MQ=255) cACGGGCCGGCAAGATCAGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:312091/135‑1 (MQ=255) cACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:37285/135‑1 (MQ=255) cACGGGCCGGCAAGATCAGTGGAAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:326314/135‑1 (MQ=255) cACGGGCCGGCAAGATCAGTGGAAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGGGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:97210/135‑1 (MQ=255) cACGGGCCGGCAAGATCAGGGGCAACCCGTAAAA‑GCCGGCCGCCTCGTCATACTTGTCGCCTTCCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAATGTACCGCATTTtcgc < 2:304006/135‑1 (MQ=255) cACGGGCCGGAATGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:267122/135‑1 (MQ=255) cACGGGCCGGAAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:235316/135‑1 (MQ=255) cACGGGCCGGAAAGATCAGTGGCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:306853/135‑1 (MQ=255) cACGGGCCGGAAAGATCAGTGCCAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:415093/135‑1 (MQ=255) cACGGGCCGGAAAGATCAGGGCCACGCCGGAAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:280077/135‑1 (MQ=255) agggggCGGCAATATGAGGGGCTAGGCGGAATT‑GGCGGCGGCCTCGACATACTTGGCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGGTGACACCGATCAAAGGGACCGCATTTgcgc < 8:382449/132‑1 (MQ=255) aCGGGCCGGCAATATCCGTGGCACGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTc < 6:113422/136‑1 (MQ=255) cGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTc < 2:316606/135‑1 (MQ=255) cGGGCCGGCAAGACCCGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTc < 4:207043/135‑1 (MQ=255) ggcggccAAGATCCGTGGAAAGCGGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:162961/125‑1 (MQ=255) ggCGGGAAAGATCAGTGGCAAGCCGGCAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:68079/131‑1 (MQ=255) ccGGAATGATCTGTGCCAACCCGCAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATggg < 6:6369/136‑1 (MQ=255) gATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca > 2:14348/1‑135 (MQ=255) aTCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTacac > 8:153051/1‑135 (MQ=255) gTGGCAAGCGGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 8:388195/134‑1 (MQ=255) gTGGCAAGCCGGCAAT‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 7:71000/134‑1 (MQ=255) gTGGCAAGCCGGCAAA‑GCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCACTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 7:106834/134‑1 (MQ=255) cAAGCCGGAAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCa < 7:11496/135‑1 (MQ=255) ccGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGAGCTCATGGGCTTACACCGTGTGCAGGTa > 2:256409/1‑135 (MQ=255) cGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTa > 1:213780/1‑134 (MQ=255) cGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAc > 5:363075/1‑135 (MQ=255) | GCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGCAAGATCAGTGGCAAGCCGGCAAA‑GCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGGTAC > ACB143/6043069‑6043327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |