| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 1,835,796 | 1 bp→TT | coding (2054/2754 nt) | barA ← | Signal transduction histidine‑protein kinaseBarA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,835,795 | 1 | . | T | 94.3% | 105.7 / ‑0.5 | 35 | coding (2055/2754 nt) | barA | Signal transduction histidine‑protein kinaseBarA |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (19/14); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | ACB143 | 1,835,796 | 0 | G | T | 94.3% | 117.2 / NA | 35 | T685N (ACC→AAC) | barA | Signal transduction histidine‑protein kinaseBarA |
| Reads supporting (aligned to +/- strand): ref base G (2/0); new base T (19/14); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑G‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCT > ACB143/1835665‑1835872 ||| aGGCGCGGCCGTCCATGCCTGCCAACTGCACATCCATCACCACCAGGTAGAACAATTCTTCCTCCACGGCCACTACCGCCGCATAACCATTGATGACGGCCAGTACCTCGGCACCCACGTCATCGAGCACG‑t‑ttt > 7:312487/1‑135 (MQ=255)aGGCGCGGCCGTCCATGCCGGGCATCTGCACCTCCATCAGCACCAGGTCGAACAAATATTCCTGCCCCGCCAGTACCCCCGCATAACCATTGTTGACGGCCCGTACCTCGGCCCCCAGGTCTTCGAGCAGG‑G‑tttt > 6:56146/1‑136 (MQ=255)aGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCCCCCAGGTCTTCGAGCAGG‑t‑ttt > 7:1419/1‑135 (MQ=255)aGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑t‑ttt > 3:209038/1‑135 (MQ=255) cgGTCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑t‑tttttta > 2:85536/1‑132 (MQ=255) cgGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑t‑ttttttac > 8:279412/1‑132 (MQ=255) tccGTCCATGCCGGTCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACcag < 6:367409/135‑1 (MQ=255) ccGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑t‑ttttttacca > 2:302268/1‑129 (MQ=255) gTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCCCCCAGGTCTTCGAGCAGGTT‑TTTTTACcagca > 8:75482/1‑135 (MQ=255) gTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCCCCCAGGTCTTCGAGCAGG‑GTTTTTTACcagca > 4:66165/1‑135 (MQ=255) gTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACcagcag > 6:89114/1‑136 (MQ=255) gTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACcagcag > 6:204218/1‑136 (MQ=255) gTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACcagca > 7:155565/1‑135 (MQ=255) tCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACcagcag < 4:342692/135‑1 (MQ=255) gCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTggcg < 3:264755/135‑1 (MQ=255) aTCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTggcggc > 1:261459/1‑135 (MQ=255) cacaTCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTgt > 4:197834/1‑135 (MQ=255) acaTCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTgt < 3:397818/134‑1 (MQ=255) catccatcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTgtcg < 8:128397/135‑1 (MQ=255) catccatcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTATTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTgtcg < 3:110746/135‑1 (MQ=255) atccatcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTgtcgtc > 6:324013/1‑136 (MQ=255) ccatcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGtcgtcg < 7:3218/135‑1 (MQ=255) ccatcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGtcgtcg < 1:272748/135‑1 (MQ=255) catcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGa > 8:235563/1‑135 (MQ=255) catcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGa > 4:119907/1‑135 (MQ=255) tcAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACg > 6:171923/1‑135 (MQ=255) gTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACTCAGAGTAgtt > 8:89509/1‑135 (MQ=255) gTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTAttt > 5:88943/1‑135 (MQ=255) gTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTAtt > 3:129561/1‑134 (MQ=255) cGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTc > 8:147580/1‑135 (MQ=255) cGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCg > 5:20837/1‑136 (MQ=255) cGAACAATTATTCCTGAAAGGCCTGTACCGAGGCATAACAATTGTTGACTTCCAGTACCTCGGCACCCAGGTCTTAGCGCAGGTT‑TTTTTACACGCAGAAGGTTGGCGGCATTGTAGTCCACGCTCAGTAgttta > 8:360117/1‑134 (MQ=255) aaTTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGgtcg > 1:366441/1‑135 (MQ=255) aaTTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGgtcg > 1:232613/1‑135 (MQ=255) ttcttcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGgtcgtc < 7:67977/135‑1 (MQ=255) tcttcCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGtcgtcg < 1:338652/135‑1 (MQ=255) ccTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTgggg < 7:112357/135‑1 (MQ=255) cTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACTCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGt < 1:227183/135‑1 (MQ=255) aCCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGATTGCCGTTGGGGTTGCt < 3:34890/135‑1 (MQ=255) tCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACg < 7:349048/52‑1 (MQ=255) tCTTCGAGCAGGTT‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACg > 8:349048/1‑52 (MQ=255) ||| AGGCGCGGCCGTCCATGCCGGGCATCTGCACATCCATCAGCACCAGGTCGAACAATTCTTCCTGCACGGCCTGTACCGCCGCATAACCATTGTTGACGGCCAGTACCTCGGCACCCAGGTCTTCGAGCAGG‑G‑TTTTTACCAGCAGCAGGTTGGCGGCATTGTCGTCGACGCAGAGTATTTTCGGTCGTCGCTGGCCGTTGGGGTTGCT > ACB143/1835665‑1835872 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |