| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 690,173 | 0 | G | T | 47.4% | 3.7 / 13.3 | 19 | intergenic (+50/‑2409) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base G (6/4); new base T (9/0); total (15/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.67e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAGGCGCTGAAACT > ACB143/690043‑690308 | aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCA‑GGCCAGCCCCC‑GGTG‑TTTTTtgggggg > 2:82998/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGGGGGC‑GGCCGGCCCCC‑CTTG‑TTTTTTTGGGGTGgtggtt > 6:267547/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GGCCGCCCCCCGTTGG‑TTTTTGGGGGTGGgttgt > 7:99045/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCA‑GGGCAGCCCGC‑GTTT‑TTTTTTTGGGTTTggtgtt > 7:86645/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GGCCTCCCCGC‑GTTG‑TTTTTTGGggttgggtttt > 6:151279/1‑132 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCC‑C‑GGTG‑TTTTTTGGGGGTGgtttttt > 5:109034/1‑136 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GGGCGGCCCCC‑GGTGTTTTTTGGGGGTTGgtttttt > 1:42187/1‑136 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GGGCGCCCCGC‑GGTT‑TTTTTTGGGGGTGggtgtttg > 5:222530/1‑135 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCCTGAGGGCCCCCCCCG‑GTTG‑GTTTTGGGgggtggtgttgg > 5:172305/1‑132 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCA‑GGCCTCCCCGC‑GTTT‑TTTTTTGGGGGTGGttgttgg > 4:254034/1‑136 (MQ=255) cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGCCCTCA‑GGCCCCCCCCC‑CTTG‑TTTTTTGGGGGTGGGTGTTGGGGGTgggggg > 5:310405/1‑135 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGGGGGCG‑GGCGGCCCCGC‑GGGG‑TTTTTGGGGGGGGGTTGTT‑TTGTTGggggggggggg > 5:7845/1‑132 (MQ=255) cAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GGCCGCCCCCC‑CTTG‑TTTTTTGGGGGTGGGTGTTTGGGGTGGGGGGGGACTGGTTTTTTGGGGTGTTGGGGGGGGGATgtgtgc > 2:205373/1‑135 (MQ=255) gggAAGTAGATGAAGC‑GGGGGGGCCGTGC‑GGCCGCCCCCC‑GGTT‑TTTTTTGGGGGTGGTTGTTGGGGTTGGGGGGGGACTGTTTTTAGAGGGTGGTTGGTGTGTGCTTTTTCCCCCCCACCCCGTGGAGTGCCagtt > 2:46945/1‑136 (MQ=255) cccccGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGGGCCTGTGAGAGCGAGCGCCGCCCGCGCGGCGCTCGGtctc < 6:222530/136‑1 (MQ=255) gC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAgg > 4:144199/1‑137 (MQ=255) gtG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGATATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAGgcgc < 4:214443/136‑1 (MQ=255) g‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCGCGGTCTCACAGgcgcgg > 5:1072/1‑135 (MQ=255) tGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAGGCGCTGAAACt < 6:71967/137‑1 (MQ=255) tGGGGGTTGGTTGTTGCGGTTGGGGAGGAACTGTATTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAGGCGCTGAAACt < 2:81212/137‑1 (MQ=255) | ACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAGGCGCTGAAACT > ACB143/690043‑690308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |