Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,383,766	0	G	T	38.7%	28.9 / 13.6	31	intergenic (+536/‑322)	CJ019_05064/CJ019_05067	putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (3/16);  new base T (12/0);  total (15/16)
Fisher's exact test for biased strand distribution p-value = 3.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGG  >  ACB143/5383631‑5383895                                                                                                                                         |                                                                                                                                  tCGGGCTGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCTCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTtgt                                                                                                                                  <  8:87238/137‑1 (MQ=255)                      cAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTTTTTTTTGGGGGGGATTTTGGCGGTGCg                                                                                                             >  4:207541/1‑137 (MQ=255)                         gaGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGt                                                                                                          <  5:85280/136‑1 (MQ=255)                            acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGACGACGCCCTCGGGCGG‑GGTTTTTTTTGGCTGGAATTTTGGGGGTGCGGGTAGa                                                                                                       >  2:245319/1‑137 (MQ=255)                                   tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTTTTTTTTGGCGGGAATTTGGGCGCGGCGGGGCGAAAAAtag                                                                                                >  5:269114/1‑135 (MQ=255)                                   tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGGTTTTTTTGGCGGGAATTTTGGGGCGGCGGGTCGAAAAAtat                                                                                                >  2:323183/1‑135 (MQ=255)                                        aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTGTGGCTGGAATTTTGGCGCTGGGGGTCGAAAAATCGGAttt                                                                                           >  6:85280/1‑137 (MQ=255)                                          ttagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                         <  6:197686/136‑1 (MQ=255)                                            agtaCCGACAGCGGAGTACTGGCGGACGGGGGATCAAATCCCCCCGGCTCCACCAAATCCCACGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                       <  8:136284/137‑1 (MQ=255)                                             gtaCCTACAGCGGAGTACTGGCGGACGGGGGATCAAATCCCACCGGCTCCACCAAATCCAAAGAAGAAGACGACCTCGGGCGT‑GGTTTTTTTTGCCTGGAAATTTGGGGCTTCGGGTCGAAAAAAACGATTTAAcac                                                                                      >  8:311045/1‑135 (MQ=255)                                             gtaCCGACAGCTGAGTACTGGCGGACGGCGGTTCAAATCCAACCGGCTCCACCAAAAACAAAGAAGAAGACGCCCTCGGGCGTGATTTTTTTTTGCGGGGAGTTT‑GGAGCTGGGGGTGCAAAAAACAGAATTAAcca                                                                                      >  6:62718/1‑136 (MQ=255)                                             gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTTGGGGGGATTTGGGGGGGGCGGGGGGAAAAAACGGtttttaacc                                                                                      >  4:170527/1‑132 (MQ=255)                                             gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAccc                                                                                      <  2:254190/137‑1 (MQ=255)                                               aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTGTGCCTGGAATTTTGGCGGGGGGGGTGGAAAAATAGGTTTTaaaacca                                                                                    >  7:105067/1‑132 (MQ=255)                                                      gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTTTTTTTTTCGTGGAATTTTGTGGCGGCGGGGGGAAAAAAACGATTTTAACCCTTTGtttt                                                                             >  6:127192/1‑135 (MQ=255)                                                         gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAgg                                                                          <  4:295224/137‑1 (MQ=255)                                                             aCTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGCGGGGTTTTTGTGGCGTGGGGGCGAAAAAACGGCTTTATCCCCATTCTTTTattgggg                                                                      >  8:31121/1‑131 (MQ=255)                                                                ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                   <  1:297823/137‑1 (MQ=255)                                                                ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                   <  3:26707/137‑1 (MQ=255)                                                                ggCGGACGGGGGCTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                   <  3:252877/137‑1 (MQ=255)                                                                      cGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCACAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAg                                                             <  6:258197/137‑1 (MQ=255)                                                                                aaTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATActgcctg                                                   <  6:151856/137‑1 (MQ=255)                                                                                                        aaaGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGGTGGTATTTGGGGGCGGCGGGGGGGAAAAACGGGTTTTAACCCGTTGTTGTTGGGGGGTTGCCAAAGGTACTGCCTGCGCCGGTTTTAAGTTggggggatt                           >  4:115568/1‑132 (MQ=255)                                                                                                        aaaGAAGAAGACGCCCTCGGGCGG‑GGTTTTTTTTTGCTGGAATTTTGGGGCGGCGGGGGGAAAAAACGGGTTTAACCCCGGTGTTGGTGGGGGGGTCCCAAACATACTGCCTGCGCCGGGTTTGCGGTGGGGGCAtt                           >  8:202513/1‑137 (MQ=255)                                                                                                           caagaagaCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTGTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                        <  4:186485/136‑1 (MQ=255)                                                                                                                agaCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg                   <  2:24917/137‑1 (MQ=255)                                                                                                                  aCGCCCTCGGGCGT‑GTTTTTTTTTGGCTGGAATTTGGGCGCTGCGGGTCGAAAAATAGGATTTTACCCCTTTGTTGTAGGGCGTTTGCCCAAGATAATGCCAGCGCCTGTTTTACGTTGGGGTCATTTTTTGAGGGt                 >  6:157535/1‑137 (MQ=255)                                                                                                                    gCCCTCGGGCGT‑GGTTTTTTGTGGCGGGAATTTTGGCGCTGCGGGGGGAAAAATCGGATTTAACCCCTTTATTTTAGGGGGTTTCCCAAAGATAATGCCTGCGCCTGTTTTCAGTTGCGGTCATTTTTTAAGGGttt               >  4:237276/1‑137 (MQ=255)                                                                                                                         cGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtc          <  3:207541/137‑1 (MQ=255)                                                                                                                          gggCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct         <  2:260299/137‑1 (MQ=255)                                                                                                                          gggCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct         <  6:37514/137‑1 (MQ=255)                                                                                                                                  tttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTggg  <  6:269114/137‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  TCGGGCGGCTGCGGATTAAATCAGTAGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGG  >  ACB143/5383631‑5383895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑