| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,811 | C→T | intergenic (‑820/+831) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,811 | 0 | C | T | 85.4% | 244.2 / 30.3 | 96 | intergenic (‑820/+831) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (3/11); new base T (28/54); total (31/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACC > ACB143/5386682‑5386943 | taGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCa < 3:14551/136‑1 (MQ=255)gAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCa < 3:86945/137‑1 (MQ=255) gTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGGTTGTTTGTCGGATCAag > 8:30278/1‑137 (MQ=255) tttCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAagag > 8:84616/1‑137 (MQ=255) tttCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAagag > 5:72077/1‑137 (MQ=255) tCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCt > 6:16858/1‑137 (MQ=255) ttCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTAcc > 7:11167/1‑137 (MQ=255) cACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAAc < 1:100718/137‑1 (MQ=255) gTCAGACCCCTTATAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTATGCGCGTAATCTGATGATTGAAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCCGATCAAGAGCTACCAACTCTTTTTc < 1:324442/137‑1 (MQ=255) gTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTc > 8:53255/1‑137 (MQ=255) gTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTc > 1:28218/1‑137 (MQ=255) aCCCCGTAGAAAAGATCAAAGGATCGTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCtttt > 8:233945/1‑130 (MQ=255) aCCCCGTAGAAAAGATCAAAGGATCCTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCtttt < 7:233945/130‑1 (MQ=255) ccccGTAGAAAATATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCTCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAgg < 2:190337/137‑1 (MQ=255) ccccGTAGAAAAGATCAAAGGATCTTCGTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAgg < 6:44974/137‑1 (MQ=255) cccGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGt > 3:143904/1‑137 (MQ=255) ccGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTa < 6:157298/137‑1 (MQ=255) ccGTAGAAAAGATCAAAGGATCTTCTTGACATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAACACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTa < 4:219052/137‑1 (MQ=255) aagTATAAAAGATCAAAGGATCTTCTTGATATGCTTTTTTTCTTCGCGTAATCTGCTGCTTTCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTAGCAACTCTTTTTCGGATGGTa < 6:78888/135‑1 (MQ=255) gTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAc > 3:257124/1‑137 (MQ=255) tAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCTTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGATCTACCAACTCTTTTTCCTAAGGTAACt < 2:220159/137‑1 (MQ=255) aGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAAACAACCGCTACCAGCGGTGGTTTGTTTGTCGGATCATGAGCTACCAACTCTTTTTACGAAGGTAACt > 8:315534/1‑137 (MQ=255) aGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTg > 4:26275/1‑137 (MQ=255) gAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTgg < 3:72020/137‑1 (MQ=255) caaacGATCAAAGGATATTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGATTGCAAAC‑AAAAAAACCACCGCTACCTGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTgg < 7:81153/136‑1 (MQ=255) aaaaGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGc > 4:127483/1‑137 (MQ=255) aaGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCtt < 2:270501/137‑1 (MQ=255) aGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTc < 3:168896/137‑1 (MQ=255) aaaGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTcagcag > 4:277137/1‑137 (MQ=255) aaaGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTcagcag > 7:58665/1‑137 (MQ=255) aaaGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATAAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTcagcag > 4:129400/1‑137 (MQ=255) aGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCagag < 3:176906/137‑1 (MQ=255) ggATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgc < 1:204379/137‑1 (MQ=255) taTCTTCTTGAGATCCATTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 5:25491/136‑1 (MQ=255) gATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 8:145830/137‑1 (MQ=255) gATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 5:276629/137‑1 (MQ=255) gATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 3:157231/137‑1 (MQ=255) gATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 3:267291/137‑1 (MQ=255) gATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAgcg < 1:235875/137‑1 (MQ=255) attGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATa < 3:258626/136‑1 (MQ=255) tagaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCa < 8:23139/136‑1 (MQ=255) agaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCaa > 3:201207/1‑137 (MQ=255) gaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCaaa < 3:64266/137‑1 (MQ=255) gaTCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCaaa < 8:193673/137‑1 (MQ=255) tCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGCGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa > 4:68579/1‑137 (MQ=255) tCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa < 4:257124/137‑1 (MQ=255) tCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa < 6:324640/137‑1 (MQ=255) tCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa < 2:179744/137‑1 (MQ=255) ccTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATAc < 2:111624/137‑1 (MQ=255) cTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCttttt > 5:138539/1‑95 (MQ=255) cTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCttttt < 6:138539/95‑1 (MQ=255) cTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACt > 3:281852/1‑137 (MQ=255) ttttttCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGt > 7:47862/1‑137 (MQ=255) tttttCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGtt < 7:16615/137‑1 (MQ=255) ttttCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttc < 2:199137/137‑1 (MQ=255) ttttCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGttc < 3:26275/137‑1 (MQ=255) ttttATTCGCGTAATCTGCTGCTTGAAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTATGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGATTCAGCAGCGCGCAGATACCAAATACTGttc < 1:93437/137‑1 (MQ=255) tGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTa < 7:84616/137‑1 (MQ=255) cgTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGTTAACTGGATTCAGAAGAGAGCAGATACCAAAAAATGTTATTATAgtgt > 4:249029/1‑137 (MQ=255) cgTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtgt < 7:260507/137‑1 (MQ=255) cgTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAgtgt > 7:280791/1‑137 (MQ=255) gTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTa > 7:222889/1‑137 (MQ=255) gTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTa < 8:183170/137‑1 (MQ=255) gTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTCGTTTGATAGTCGCATCAAGAGCAACCAACTCTTTTTCCGAAGGTAACTGGCTTCATCAGAGCGCAGATACAAAATAATGTTCTTCTACTGTa > 7:180191/1‑137 (MQ=255) ctgctgCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGt < 4:201207/137‑1 (MQ=255) gctTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTa < 4:68544/80‑1 (MQ=255) gctTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTa > 3:68544/1‑80 (MQ=255) ctTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAg < 5:16858/137‑1 (MQ=255) tGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGc < 8:263760/137‑1 (MQ=255) tGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGc < 3:285582/137‑1 (MQ=255) gCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc < 6:116986/137‑1 (MQ=255) gCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc < 6:111583/137‑1 (MQ=255) gCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc < 3:127634/137‑1 (MQ=255) gCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGcc < 7:297719/137‑1 (MQ=255) aaac‑aaaAAAACCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGccac < 7:87678/137‑1 (MQ=255) aaaaaaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa > 7:167504/1‑100 (MQ=255) aaaaaaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATa < 8:167504/100‑1 (MQ=255) aaaaaaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACtt < 8:280791/137‑1 (MQ=255) aaaaaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTc < 3:277137/137‑1 (MQ=255) aaaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa < 3:63162/137‑1 (MQ=255) aaaCCACCGCTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCaa < 1:290781/137‑1 (MQ=255) cTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCcaccac < 1:324503/123‑1 (MQ=255) cTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCcaccac > 2:324503/1‑123 (MQ=255) cTACCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGt > 2:204375/1‑137 (MQ=255) aaCCTGCGGTGGTTTGTTTGTCGGATCAAGAGCTAACAACTCTTTTTCCTAAGTTAACTGGCTTCAGCAGATCGCATATACCAAATACTGTGCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTa < 3:203143/136‑1 (MQ=255) aCCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg < 7:53255/137‑1 (MQ=255) aCCAGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAAATGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAATAACTCTGTAg < 7:252800/137‑1 (MQ=255) aCCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAg < 2:60821/137‑1 (MQ=255) aGCGGTGGTTTGTTTGTCGGATCAAGAGCTACCCACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCAc < 5:4776/137‑1 (MQ=255) cGGTGGTTTGTTTGTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCg < 8:11167/137‑1 (MQ=255) ggtggtTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAAAACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGc > 1:223325/1‑137 (MQ=255) tttgtttgTCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACa < 6:72077/137‑1 (MQ=255) tttgtttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACa > 8:92003/1‑137 (MQ=255) tttgTCGGATCAAGAGCTACCAACTCATTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc < 8:288531/137‑1 (MQ=255) tttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc < 3:205171/137‑1 (MQ=255) tttgCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATAcc < 4:102677/137‑1 (MQ=255) | GAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAAC‑AAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACC > ACB143/5386682‑5386943 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |