| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 690,144 | A→G | intergenic (+21/‑2438) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 690,144 | 0 | A | G | 87.5% | 18.9 / ‑4.1 | 18 | intergenic (+21/‑2438) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base A (2/0); major base G (14/0); minor base C (2/0); total (18/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTG > ACB143/690021‑690260 | cccGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCA‑ggggg > 3:189360/1‑134 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCA‑CGCCAccccccg > 2:37557/1‑134 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGCCGGCA‑GGGCGccccccc > 6:281331/1‑134 (MQ=255) ttCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCCTCA‑TGCCCCCCCGC‑GTTT‑ttgttt > 6:69525/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCA‑GGCCAGCCCCC‑GGTG‑TTTTTtgggggg > 2:82998/1‑135 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGGGGGC‑GGCCGGCCCCC‑CTTG‑TTTTTTTGGGGTGgtggtt > 6:267547/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GGCCGCCCCCCGTTGG‑TTTTTGGGGGTGGgttgt > 7:99045/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCA‑GGGCAGCCCGC‑GTTT‑TTTTTTTGGGTTTggtgtt > 7:86645/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GGCCTCCCCGC‑GTTG‑TTTTTTGGggttgggtttt > 6:151279/1‑132 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCC‑C‑GGTG‑TTTTTTGGGGGTGgtttttt > 5:109034/1‑136 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GGGCGGCCCCC‑GGTGTTTTTTGGGGGTTGgtttttt > 1:42187/1‑136 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGA‑GGGCGCCCCGC‑GGTT‑TTTTTTGGGGGTGggtgtttg > 5:222530/1‑135 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCCTGAGGGCCCCCCCCG‑GTTG‑GTTTTGGGgggtggtgttgg > 5:172305/1‑132 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCA‑GGCCTCCCCGC‑GTTT‑TTTTTTGGGGGTGGttgttgg > 4:254034/1‑136 (MQ=255) cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGCCCTCA‑GGCCCCCCCCC‑CTTG‑TTTTTTGGGGGTGGGTGTTGGGGGTgggggg > 5:310405/1‑135 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGGGGGCG‑GGCGGCCCCGC‑GGGG‑TTTTTGGGGGGGGGTTGTT‑TTGTTGggggggggggg > 5:7845/1‑132 (MQ=255) cAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGA‑GGCCGCCCCCC‑CTTG‑TTTTTTGGGGGTGGGTGTTTGGGGTGGGGGGGGACTGGTTTTTTGGGGTGTTGGGGGGGGGATgtgtgc > 2:205373/1‑135 (MQ=255) gggAAGTAGATGAAGC‑GGGGGGGCCGTGC‑GGCCGCCCCCC‑GGTT‑TTTTTTGGGGGTGGTTGTTGGGGTTGGGGGGGGACTGTTTTTAGAGGGTGGTTGGTGTGTGCTTTTTCCCCCCCACCCCGTGGAGTGCCagtt > 2:46945/1‑136 (MQ=255) | CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCA‑GGCCTCCCCGC‑TTTG‑TTTTTGGGGGTTGGTTGTTGCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTG > ACB143/690021‑690260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |