| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 3,063,553 | 0 | C | G | 53.8% | 5.1 / 16.0 | 13 | intergenic (+81/‑273) | adh_2/pqqD_2 | Aldehyde dehydrogenase/PqqA binding protein |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base G (1/6); total (6/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.91e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.13e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCC‑GCTCCCACAGGTTCACCGCTGGTT‑CCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGCA > ACB143/3063422‑3063683 | aCCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTTCCC‑GCCACCCCACCTTGACCCGAGGCT‑CCAGCACACGTGGAGAAACAGTGGgggccggc > 1:274349/1‑136 (MQ=12) aaGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCC‑GCCCCCCCCAGTTGACCCCCGGGT‑CCAGCACCCGGGTAACACCCGTGGgggcggggccg > 3:40400/1‑132 (MQ=17) gTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCATGCCC‑CCTCCCACACGGTCACCCCGGGGT‑GCAGAACCCGTGAAAAAACCGGGTGGGGGGGGGAGCCCCCAAAAagg > 5:152922/1‑136 (MQ=16) caCATCAATCCGCGGGGCTCCTTCTAAACCGCGGCGCGGCCTTCGCGGGCTGGCCC‑GCTCCCACAGGTTCACCGCTGGTT‑CCACAACCGGTGAATAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCAcat < 5:317999/135‑1 (MQ=255) actataaTCGGCTGGGCTTTTTATAAACCGCGCCGCGG‑CGTCGGGGGGTTGCCCGGCGCCCACATGTTC‑CCGGGGTTTGCCTCACCGGGTTAACACCGTGGGGGGGGGGGCGAGCCCGCAAAAGGGCCGGCAcat < 6:152922/130‑1 (MQ=255) cttcaAACCCGCGGCGCGGCGTGGGGGGGTTGGCCC‑GCCCCCACCGGTGCACCGCTGGTC‑CCAAACCGGGTAAATACCGTGTGGGGGGGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCa < 5:276951/135‑1 (MQ=255) tctAACCCGGGGCGCGGCCTTCGGGGGCTGGCCC‑GCCCCCACTGGTTCACCGCGGTTG‑CCACAACCGGTTAATAACCTGTGGGGGGGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAAc < 7:326975/135‑1 (MQ=255) caccGGTTCCCCGCTGTTT‑CCACAACGGGTTAATAACGTGTGGGGGGGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGt < 6:326937/136‑1 (MQ=255) gCTGGTT‑CCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCGACCCGTACCAACGCACCCCGCACGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGc > 7:330860/1‑136 (MQ=255) t‑CCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAAt > 7:23894/1‑136 (MQ=255) cAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGc > 4:144872/1‑136 (MQ=255) gtgGGGGGGGGCTAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACTCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCgg < 5:139375/136‑1 (MQ=255) tgGGGGGGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCgg < 3:336616/135‑1 (MQ=255) gggggCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGca > 3:115256/1‑136 (MQ=255) ggggCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGca > 4:56505/1‑135 (MQ=255) | ACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCC‑GCTCCCACAGGTTCACCGCTGGTT‑CCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGCA > ACB143/3063422‑3063683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |