| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,383,758 | 0 | C | G | 50.0% | 22.7 / 15.8 | 27 | intergenic (+528/‑330) | CJ019_05064/CJ019_05067 | putative protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/13); new base G (13/0); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTC > ACB143/5383658‑5383889 | acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTGTTTTGGGGCTGGAATTTGGGCGCGGCGGGTCg > 8:183453/1‑136 (MQ=255) aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGGTTTTTTTTGGGGGGATTTGGTGGCGGCGGGGCGaaaaaaag > 2:27665/1‑133 (MQ=255) aTGTCGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTAGGCGCTGCGGGTCGAAAAATCGGAtt < 1:318447/136‑1 (MQ=255) gtccccaCAGCGGAGTACTGGCGGACGTGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGCCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc < 8:139341/130‑1 (MQ=255) gtaCCTACCGCGGAGTCCGGGCGGACGGGGGTTCAAAACCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc < 6:211110/136‑1 (MQ=255) gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTTGCTGGAATTTTGGGGCTGGGGGGGGAAAAATAGGGTTTAAcc > 8:16093/1‑136 (MQ=255) gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTGTGGCTGGGATTTGGGGGCGGCGGGGGGAAAAAAAGGTTTTAAca > 6:337628/1‑135 (MQ=255) gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc < 8:103552/136‑1 (MQ=255) gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc < 2:28022/136‑1 (MQ=255) ggaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAACAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc < 4:205289/134‑1 (MQ=255) aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGGGGGGATTTTGGG‑GGTGCGGGTCGGAAAATAGGGTTTaaacac > 1:2637/1‑132 (MQ=255) aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GTTTTTTTTTGCCTGGAATTTTGGCGCTGCGGGTCGAAAAATAGGATTTAAaccc > 7:22014/1‑136 (MQ=255) aGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCACAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCtt < 3:255991/136‑1 (MQ=255) gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGCGGGGATTTTTGGGCGGGGGGGGGCAAAATCGGATTTAACACCGTTCTTg > 6:342961/1‑136 (MQ=255) gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGCGGGAATTTGGGGGCTGCGGGTGGAAAAATAGGATTTTACCCCGTTCTTg > 1:235610/1‑136 (MQ=255) aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCGCGGGCGTGGTTTTTTTTTGCTGGGATTTTGGGGGGGGGGGGGGGAAAAA‑AGGAGTTAAACCCGTtgttgtttt > 6:112486/1‑133 (MQ=255) gTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg < 6:343800/136‑1 (MQ=255) ttCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGCTGGGATTTTGGGGGGGGGGGGGGGAAAATAGGTATTAAACCCGGTGTTTTTGGGCGTTTGCCaaaaaaaat > 2:28280/1‑130 (MQ=255) aTCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTGTGGCGGGGATTTGGGGGGGGCGGGTCGAAAAATAGGGTTTAAACCCTTTCTTGTATGGCGGTTGCCAAAGATActgcctg > 1:32192/1‑136 (MQ=255) tCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑GGGTTTTTTTTGGGGGCATTTTGGGGGTGCGGGGGGGAAAATACGATTTAAACACAATGATGTAGGGCGTGTGCCCAAAATAatggaaaa > 2:97245/1‑131 (MQ=255) aaaGAAGAAGACGCCCTCGGGCGT‑GGTTTTTTTTGGGGGGGATTTTGGGGCGGGGGGGGGTAAAATAGGATTTTAACCCGTTGTTGTTGGGGGTTTTCGAAATATAATTCCTGCGCCGGTTTTAAGTTGGGGTGAt > 4:173363/1‑136 (MQ=255) aagaagaCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 3:187884/136‑1 (MQ=255) aagaagaCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 1:28280/136‑1 (MQ=255) agatgaCGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt < 2:142702/135‑1 (MQ=255) gCCCTCGGGCGT‑CTTTTTTTGTGCCTTGACTTTGGGCGCTGCTGTTCGAAAAATCTGATTTAACCCCTTTATTGTAGGGCGTTTCACAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtt < 8:310995/136‑1 (MQ=255) tcGT‑ATTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGTTACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc < 8:261056/135‑1 (MQ=255) gCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGTGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGGTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc < 8:261053/136‑1 (MQ=255) | ACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGT‑CTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTC > ACB143/5383658‑5383889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |