| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,300,670 | T→G | 45.8% | intergenic (‑100/+3473) | recC ← / ← PP_RS08750 | RecBCD enzyme subunit RecC/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,300,670 | 0 | T | G | 45.8% | 1.6 / 3.0 | 27 | intergenic (‑100/+3473) | recC/PP_RS08750 | RecBCD enzyme subunit RecC/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (9/4); new base G (7/3); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACT > ACB143/5300555‑5300798 | gggCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGGGC‑GGCCGTGCCCGCTCCCGCAGCGCTTggcggcgg > 2:15691/1‑132 (MQ=255) gCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGGGC‑CGCCGTGCCCGCTCCCGCCGCGCTTGGCGCAgggc > 4:66887/1‑134 (MQ=255) gCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCGCCCGCCGCGCTTCgcgccagtc > 4:70969/1‑131 (MQ=255) cGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑CGGGGC‑GGGCCTGCCCGCGCCCCCAGCGATTCGCGCAGGTCg > 4:157804/1‑136 (MQ=255) ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGCGC‑GGGCGGGCCCCCTACCGCATCGATTCGCCCAGGtg > 4:44313/1‑133 (MQ=255) ggTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCTCCCGCAGCGATTCGCGCAgggc > 8:82002/1‑132 (MQ=255) aGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGCGC‑GGGCGTGCCCGCTGCCGCAGCGCGTCGGGGAGGGCGA‑G‑gttttt > 6:202858/1‑136 (MQ=255) aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCGCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCcaca > 8:42981/1‑135 (MQ=255) aaGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCGCCCGCACCGATTCGCGCAGGTCGA‑G‑GTTTTTTCCcaca > 3:244204/1‑135 (MQ=255) ggTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGCGGGGCCC‑GGCCG‑GACCCCGCCCGCAGCCATAAGCGCACCGCGA‑G‑GTTTTTGGCCACaaa > 6:200027/1‑135 (MQ=255) ggTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCcaca > 8:63102/1‑133 (MQ=255) tGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGTGC‑GGCCGGGCCCCCTGCCCCCACCATTGGCGCCGGTGGC‑GTTTTTTTGCCCCCAAA‑ACAAAAacccaaa > 5:272416/1‑131 (MQ=255) tGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGGGCGGGCCGGGCCC‑CACCCCCCGCGAATGGCGCCGGGGGACG‑TTTTTTGCCCCCAAAACCAAAAcccaaccc > 5:276348/1‑131 (MQ=255) tGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGGGC‑GGGCGTGCCCGCTCCCCCAGCGCTTAGCGCAGGTCGA‑G‑TTTTTTTCCCACAAAGACAAAAACCCAAc > 1:156316/1‑134 (MQ=255) aTGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGGGCCCCCCCCCCCCGCGAATAGCGCAGGTCGG‑G‑TTTTTTTCCCCCAAACAAAAAAACACAACCttc > 8:9107/1‑134 (MQ=255) gCTGAGAGGGCAACGATATCACGCGTACATGAACCGGCCGAATCCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCGCCCGAAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCtgcgt < 8:146084/135‑1 (MQ=38) gATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGGGC‑GGCCGTGCCCCCGCCCGCCGCGATTAGCGCAGGGGGA‑G‑TTTTTTTCCCACAAAGACAAAACCCCGACCTGCGGGCGCAGATATGGGtt > 1:77262/1‑136 (MQ=37) aCGCGTACTGTAACCGGCCGAATGCCATCAGGCGGC‑GGGCGC‑GGCCGGGCCCGCGCCCGAAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGa < 6:125972/136‑1 (MQ=21) gTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGCGC‑GGCCGGGGCCCCGCCCCCCCCGCATGGCGCAGGGGGA‑G‑GTTTTTTGCCACAAAAACAAAACCCCAAACAGCCTGCGCAAATAAGGGTTTTTCGGAAtt > 3:138600/1‑135 (MQ=255) caaaCGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCGCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAACGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCt < 5:280953/134‑1 (MQ=255) gCCTAATGCATTCAGGCTGC‑GGGAGC‑GGCGGTGCCCGCTCCGGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGAt < 8:209370/136‑1 (MQ=21) gCCGAATCCAATCAGGCTGC‑GGTAGC‑GGCCGGGCCCGCCCCCGAAGCGATTCGCGCAGGTAGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGAt < 5:226696/136‑1 (MQ=18) gCCCAATGCCATCTGGGTGC‑GGGAGC‑GGCGGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGAt < 6:69367/136‑1 (MQ=255) cccATCCGGCTGC‑GGTCGG‑GGCGGGGCCGGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTAc < 8:271809/135‑1 (MQ=17) c‑GGCCGTGCCCGCTCCCGAAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAAccc < 7:63102/136‑1 (MQ=255) gggccccGCCCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCcaca < 3:218168/132‑1 (MQ=255) gCCCGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCGACTCTCACATGGGGAAACCCCACACt > 3:234640/1‑135 (MQ=255) | GGGCGGTGTTGAGCATCGGAAGGTCTCTGGAGGCGCTGATGGCTGAGAGGGGAACGATATCACGCGTACATGAAACGGCCGAATGCCATCAGGCTGC‑GGGAGC‑GGCCGTGCCCGCTCCCGCAGCGATTCGCGCAGGTCGA‑G‑TTTTTTGCCCACAAAGACAAAACCCCTACCTGCGTGCGCAGATAGGGGTTTTGCGAAATGAATCTTGACGATGACCTACTCTCACATGGGGAAACCCCACACT > ACB143/5300555‑5300798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |