Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,693,175	A→C	54.2%	S236R (AGC→CGC)	pyrC' →	Dihydroorotase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,693,175	0	A	C	54.2%	‑4.2 / 18.8	44	S236R (AGC→CGC)	pyrC'	Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (7/13);  new base C (3/21);  total (10/34)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAG  >  ACB143/5693041‑5693306                                                                                                                                        |                                                                                                                                    tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGCGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACGTCCGCCAGCTAACCAg                                                                                                                                    >  5:57455/1‑136 (MQ=255)    cccACGATGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAgcgc                                                                                                                                 <  3:101028/136‑1 (MQ=255)                 cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCGCCCGATCACCCGCCCCCGTGGCGCGCgg                                                                                                                    >  2:5143/1‑136 (MQ=255)                       cAGCTTCCTGGGCCTGCCGGGCATCGCGGAGAGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGGGGGCGTGCGCACTTCAGCCAGACCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                              <  1:298654/136‑1 (MQ=255)                           ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCCGATCACCCGCGCCCGGGGCGCGCGGCGGCTCgccc                                                                                                          >  3:37995/1‑136 (MQ=255)                             ccTCGGCGTGCCGGCCCTCCGGGAGCGCCCCGAACCCGGGGCCCGGGCGCGCAACCTGG‑TGCTGGTGGAACTCTGCGGGGTGCTGGCGCACTTCAGCCAGACCCCCCGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                        <  4:229993/136‑1 (MQ=255)                             ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGGGCGCCCATAAGCCCACTAACCACCGCCCCGGGCGCCCGGCAGCTAgcccac                                                                                                        >  2:155245/1‑135 (MQ=255)                               gcgccgtGCGGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCGGGCGCGAAACCTGC‑TGCTGGGGCAACTGAGCGGCGTGCGTGCGCACTTCAGCCACACCCCCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                                      <  2:56411/130‑1 (MQ=255)                               gcGGCCTGCCGGGCATCCCGGAGAGCGCCGAACCGGGGGCCCGGGCGCGCAACCTGC‑TGCTGGGGGAACACCGCGGCGGGGGGGCGCACTACAGCCAGAACCCCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                      <  8:31371/135‑1 (MQ=255)                                 ggccgcccGGGCATCCCGGAGAGCGCCTAAACCGGGGCCCGGGCGCGCACCCGGC‑TGCGGGTGGAACCGCGCGGCGGGGGTGCGCAATCCCGCCAGAACACCCGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                      <  4:82272/128‑1 (MQ=255)                                 ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACGTGC‑GGCTGGTGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccaggc                                                                                                      <  5:195098/134‑1 (MQ=255)                                   ccggCCGGCCATCCCGGACCGCCCCGAACCGGGGGCCCTGGCGCGCAACCTGC‑TGCTGGGGTAACAGCGCGGGGGGCGGGCCCACTGCCGCCCGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccaggcccag                                                                                                  <  6:199519/133‑1 (MQ=255)                                         gggctgcccGGAGAGCGCCTAAACGGGGGCGGGGGCGCGCACCGTGG‑TGCTGGGGGAACCGCGGGGCGGGCGGGCGCAATCCCCCCCGACCACCCGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                            <  2:107956/130‑1 (MQ=255)                                                 cGGTGAGCGCCTAAACCGGGGCCCTGGCGCGCACCCTGC‑GGCGGGTGGAACAGAGGGGCGTGCGGGCGCACTTCAGCCCGAGCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGcc                                                                                    <  5:69698/136‑1 (MQ=255)                                                  ggAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCg                                                                                   <  7:255648/136‑1 (MQ=255)                                                       ggccgtaaaCGGGGGCCCTGGCGCG‑AAACTGCGTGGTGGTGCACCCGCGGGGCGGGCGGGGGCACTACAGCCAGACCACCCGCGCCGTGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                              <  2:266736/130‑1 (MQ=255)                                                       gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                              <  5:262354/136‑1 (MQ=255)                                                       gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCGGGTGGAACAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                              <  7:52109/136‑1 (MQ=255)                                                       gcgcCGAAACCGGGGCCCTGGCGCGCAACCTGC‑TGCTGGGGGACCAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                              <  4:223893/136‑1 (MQ=255)                                                                 gggggCCGTGGCGCGCAACCTGC‑GGGTGGGGCAACCGCGGGGGGGGGGGGCGCACTGCAGCCAGATCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  3:201062/133‑1 (MQ=255)                                                                 ggTGGCCCTGGCGCGAAACGTGC‑TGCTGGTGTAACAGAGCGGCGTGCGTGCGAACTACCGCCATATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  5:251366/135‑1 (MQ=255)                                                                 cggggCCGTGGCGCGCAACCTGG‑TGCTGGTGGAACCGCGCGGCGGGCGGGCGCAATTCACCCAGACCCCCCGCGCGCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  4:18293/133‑1 (MQ=255)                                                                 cggggCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  6:274609/133‑1 (MQ=255)                                                                 cGTGGCCCTGGCGCGCACCCTGC‑TGCGGGGTGAACAGTGCGGCGGGGGTGCGAACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                    <  2:90441/136‑1 (MQ=255)                                                                      cccTGGCGCGCAACCTGC‑TGCTGGGGTAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                               <  6:62708/136‑1 (MQ=255)                                                                             gcgcAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGCTCGCCCCGGCCCCGGCCGTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAcccg                                                        >  1:176015/1‑134 (MQ=255)                                                                              cgcAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCATAAGCCCGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCGTGGGCCTGCCGGTAACCGCCGCTGTAGCGCGGTTCCAGc                                                       >  7:21306/1‑136 (MQ=255)                                                                               gcAACCTGC‑GGCTGGGGGAACAGAGGGGCGTGGGTGCGCACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                                      <  7:78405/136‑1 (MQ=255)                                                                                                      gagCGGCGTGCGTGCGCACTTCAGCCACACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg                                <  1:37752/136‑1 (MQ=255)                                                                                                      gagCGGCGTGCGGGCGAACGGCACCCACATCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCGGTACCAGCTGATCCTCACGGACGAGTCGCTg                                <  6:84586/136‑1 (MQ=255)                                                                                                        gcggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGCTCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACCGACGAGTCGCTgcg                              >  7:78720/1‑136 (MQ=255)                                                                                                             gtgcgtgcGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  8:215020/136‑1 (MQ=255)                                                                                                             gggggtgcgAACTTCAGCCATAGCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  2:22748/132‑1 (MQ=255)                                                                                                             gggcgtgcgAAGTTCGCCCAGATCCCCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  4:294955/134‑1 (MQ=255)                                                                                                             gggcgggcgCACTCCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  2:70034/134‑1 (MQ=255)                                                                                                             gggcgggccCACTTCCCCCCGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                         <  1:139922/134‑1 (MQ=255)                                                                                                               gcgtgcgCACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATAGCCCAGGCCCCGGCCTTGGGCCTGCCGGTAACCGCCGAGGTATCGCTGTACCAGCTGATCCTCACGGACTAGTCGCTGCGCGAGtta                       >  2:81688/1‑135 (MQ=255)                                                                                                               gcgtgcgCACTTCAGCCAGACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                       <  6:203227/136‑1 (MQ=255)                                                                                                               gcgtgcgCACATCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCGTGGGCCTTCCGGGAACCGCCGCTGTAGCGCTGTACCCGCTGATCCTCACGGACGCGTCGCTGCGCGAGTtc                       >  7:150129/1‑136 (MQ=255)                                                                                                                 gggcgcACTTCAGCAAGATCACCAGCGCCCTGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtctc                     <  1:259860/134‑1 (MQ=255)                                                                                                                  tgcgCACTTCAGCCAGATCACCAGCGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                    <  8:25409/136‑1 (MQ=255)                                                                                                                  ggcgcCCGTCAGCCAGAACACCCGCGCCCGTGGCCCGCGGCTGATCGCCCAGGCCCAGTCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                    <  6:129159/135‑1 (MQ=255)                                                                                                                                 gATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTACCCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTg     >  3:184818/1‑136 (MQ=255)                                                                                                                                    cACCCGGGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg  <  3:70107/136‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGC‑TGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAG  >  ACB143/5693041‑5693306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑