| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,537,457 | 0 | T | G | 52.9% | 0.9 / 11.0 | 17 | V402G (GTG→GGG) | mdfA | Multidrug transporter MdfA |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (0/9); total (2/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTT‑TGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCC > ACB143/1537323‑1537556 | ggggATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTT‑tgtg > 7:11009/1‑136 (MQ=255) tgctgGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTT‑TGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATc > 5:105137/1‑136 (MQ=255) gCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGGT‑TGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGCTAATCGGTAt > 1:171457/1‑136 (MQ=255) gtgatttttGCCG‑TGGGGGGGGGGGGGGGGGGGGCGTTGGCGCGGTGGCCGCTGTGGTG‑TGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 7:225648/131‑1 (MQ=255) ggggtttttGCGC‑GGGGGGGGGGGGGGGTGGGGGCGTGGGCGCGGTGGGCGCTGTGGTT‑GGGGGGGGGTGGGGGGCGTGGGGAAGGGGCTGTAGCTCGTTAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 7:236196/131‑1 (MQ=255) tggcttttGCCCGGGGGGGGGGGGGGGGGGGGGGGGTTGGCGCTGTGGCGGCTGTGGTT‑TGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 5:258938/132‑1 (MQ=255) tcATTTTGCCCGGGGGGGGGGGGGGGGCGGGGCCGTTGGGGTGGCGCCGGGTGTGGTT‑GGTGGGGGGGGGCGGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaaa < 5:194464/135‑1 (MQ=255) gatttttGCCG‑GGGGGGGGGGGGGGGGGGGGGGGTTGGCGCTGTGGCCGGTGGGGTT‑TGGGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaaa < 3:7126/132‑1 (MQ=255) tttttGCCG‑GGGGGGGGGGGGGGGGGGGGGCGTTGGCGGTGTGGCGGCTGTGGTT‑TGGGGTGGGTGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAc < 3:364136/133‑1 (MQ=255) aTTTTGCCG‑TGGGGGGGGGGGTGG‑GGGGGGGTTGGCGCTGGGGCCGGGGTGGTT‑TGGGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCg < 7:58887/136‑1 (MQ=255) ggggggggggggggggggggggggCGGGGGGGCCGCTGTGGTT‑TGGGGGGGTGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca < 3:43020/131‑1 (MQ=25) ggggggggggggggggggggTGGGCGCGGGGGCCGGGGTGGTT‑TGGGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca < 3:89919/131‑1 (MQ=37) ggggggggggggggGTTTGGGGCGGTGG‑CGGTGTTGTTGTGGGGTGGGGGGGCGGCCGGGGTAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 1:177648/136‑1 (MQ=25) ggtgTGGGGGGGGGTTGGGCGTTGTGGCGTCTTTGTTT‑GGTGGGGGTTGGGGGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccg < 8:98374/133‑1 (MQ=25) ggggggggggggGCTTGGGGGCGGGGGCCGCGGGGTTT‑GGGGGTGGGCGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 4:96263/135‑1 (MQ=25) ggggggggggggGCGTTGGCGCTTGGGCCGCGGTGGTT‑TGTGGTGGGTGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgcc < 4:255057/135‑1 (MQ=38) gggggggggggcgggggggcggTGGCGGGTGGGGTG‑TGTGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcg < 6:325198/130‑1 (MQ=25) gggggggggCGTTGGGGGTTGGCCCGGTGGGGTT‑TGGTGTGGGTGGGCGGCTTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCTGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGgccgcgcc < 8:2257/132‑1 (MQ=37) | GGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTT‑TGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGGCCGCGCC > ACB143/1537323‑1537556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |