Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,500	C→A	intergenic (‑509/+1142)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,500	0	C	A	100.0%	73.2 / NA	27	intergenic (‑509/+1142)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/15);  total (12/15)

ATAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑CGT‑‑‑CA‑GGAT‑C‑‑A‑‑T‑CCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG  >  ACB143/5386368‑5386604                                                                                                                                     |                                                                                                                    aTAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtg                                                                                                                  >  1:383321/1‑135 (MQ=255)   aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTTAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               >  6:40493/1‑136 (MQ=255)   aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               >  1:88072/1‑136 (MQ=255)   aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               >  6:83234/1‑136 (MQ=255)    tccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               <  1:334795/134‑1 (MQ=255)    gCCAGCAGTCTAACTAAACAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               <  5:35648/135‑1 (MQ=255)    gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCTGTGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCGTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggtg                                                                                                              <  6:91304/136‑2 (MQ=255)    gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGTGGTGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggtg                                                                                                              <  8:293739/136‑2 (MQ=255)    gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               <  5:112699/135‑1 (MQ=255)     ccAGCAGTCTAACTAAAAAGGCCTCCCAGATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               <  4:245418/134‑1 (MQ=255)     ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggtg                                                                                                              <  4:357961/135‑2 (MQ=255)     ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggtg                                                                                                              >  6:223659/1‑134 (MQ=255)     ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggtg                                                                                                              >  6:252029/1‑134 (MQ=255)     ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggtg                                                                                                              >  7:224196/1‑134 (MQ=255)     ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               >  4:146432/1‑134 (MQ=255)     ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               <  3:361300/134‑1 (MQ=255)          agTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggtgtacgg                                                                                                         >  1:139192/1‑129 (MQ=39)                                           ccTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGc                                                                 >  8:196722/1‑136 (MQ=255)                                           ccTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGc                                                                 >  1:140318/1‑136 (MQ=255)                                                    ttGATAACAAAAATTGATCTAATTCTTTAATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGGCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa                                                         <  1:381239/135‑1 (MQ=255)                                                          aCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAc                                                   >  4:93987/1‑135 (MQ=255)                                                                 ttGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg                                            <  4:38452/135‑1 (MQ=255)                                                                               tCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                               <  8:284278/134‑1 (MQ=255)                                                                               tCATTAAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGT‑‑‑TA‑CGCTAC‑‑AACC‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt                               <  6:40934/134‑1 (MQ=255)                                                                                 aTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCGCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                           <  7:90429/136‑1 (MQ=255)                                                                                 aTTTAACACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑C‑‑AACC‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                           <  7:36010/136‑1 (MQ=255)                                                                                        aCCCTAATATGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGt                    <  1:155497/136‑1 (MQ=255)                                                                                          ccTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGCGGGCTTTTTTATTGCGCTGCGGGTGc                  >  6:291024/1‑136 (MQ=255)                                                                                              ttatGACATCGCGGCCGGCTGGTCGACGACGGGGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  2:383321/135‑1 (MQ=255)                                                                                              atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  6:139809/136‑1 (MQ=255)                                                                                              atatGACATCTCGGCCGGCGTGTCGACGACGGCGGTCGCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGAGGTTGCCAgg              <  8:255959/136‑1 (MQ=255)                                                                                              atatCACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  1:143764/136‑1 (MQ=255)                                                                                               tatGACATCTCGGCCGGCTTGGCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg              <  4:337739/135‑1 (MQ=255)                                                                                                     aTCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               <  7:104797/38‑1 (MQ=255)                                                                                                     aTCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑ggt                                                                                                               >  8:104797/1‑38 (MQ=255)                                                                                                       ctcGGCCGGCTTGTTGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCGTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt     >  5:3657/1‑136 (MQ=255)                                                                                                       ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt     >  6:362927/1‑136 (MQ=255)                                                                                                       ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt     >  1:216374/1‑136 (MQ=255)                                                                                                       ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt     >  5:69561/1‑136 (MQ=255)                                                                                                       ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTATTATTGCGCTGCGGGAGCCAGGGCGTGccc      >  3:212377/1‑135 (MQ=255)                                                                                                        tcGGCCGGCTTGTCGACCACGGCGGTCTCAGT‑GGTGTACG‑GTAC‑A‑‑A‑‑TCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGATGCGGGTGCCAGGGCGTGACCtt    >  5:313863/1‑136 (MQ=255)                                                                                                          ggccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg  >  7:312245/1‑136 (MQ=255)                                                                                                          ggccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg  >  5:132480/1‑136 (MQ=255)                                                                                                           gccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt    <  4:192186/133‑1 (MQ=255)                                                                                                           gccggcTTGTCGACGACGGCGGTCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt    <  4:348427/133‑1 (MQ=255)                                                                                                           gccggcTTGTCGACGACGGCGGGCTCAGT‑GGTG‑‑TACGGTA‑CAAA‑‑C‑CCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt    <  4:183160/133‑1 (MQ=255)                                                                                                                                     |                                                                                                                    ATAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑CGT‑‑‑CA‑GGAT‑C‑‑A‑‑T‑CCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGG  >  ACB143/5386368‑5386604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑