Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,456,028 A→C 15.5% intergenic (‑50/+92) patA ← / ← algL Peptidoglycan O‑acetyltransferase/Alginate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,456,0280AC15.5% 91.0 / 2.4 49intergenic (‑50/+92)patA/algLPeptidoglycan O‑acetyltransferase/Alginate lyase
Reads supporting (aligned to +/- strand):  ref base A (13/27);  new base C (5/4);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATAACGTTGCCCGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGC  >  ACB143/1455896‑1456137
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ctaTAACGTTGCCCGCTCAAGTAGTACAGTCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGAACATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAAcc                                                                                                               <  3:159393/133‑1 (MQ=255)
cGATAACGTTGCCCGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTTTTCACAGCATCCAGGGCAAAGCGCCCCCCAAAcc                                                                                                               <  5:393/135‑1 (MQ=255)
cGATAACGTTGCCCGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAAcc                                                                                                               <  1:151589/135‑1 (MQ=255)
cGATAACGTTGCCCGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAAcc                                                                                                               <  2:213646/135‑1 (MQ=255)
cGATAACGTTGCCCGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAAcc                                                                                                               <  3:44552/135‑1 (MQ=255)
      cGTTGCCCGCTCAAGTAGTACCTGCCGATGAAGATCGGCAAGAACAGGAACATGAACACGTTGGACGAGAAGACCATCCCGGTTCTACTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGt                                                                                                        <  2:285675/136‑1 (MQ=255)
       gTTGCCCGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTg                                                                                                       <  2:391391/136‑1 (MQ=255)
            ccGCTCAAGTAGTACAGGCCGACGAAGATCGGCAAGAACACGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAgg                                                                                                  <  8:353594/136‑1 (MQ=255)
             cGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGc                                                                                                 >  7:307727/1‑136 (MQ=255)
              gCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCCGGGCAAGGcg                                                                                                >  4:124836/1‑135 (MQ=255)
              gCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGGGCCAGGcg                                                                                                >  7:74799/1‑135 (MQ=255)
                 cAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGa                                                                                             <  4:51184/136‑1 (MQ=255)
                  aaGTAGTACAGGGCGAGGAAGATCGGCAAGAACACGAACAGGAACAGGTTGGACGAGTAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCACGGCAAAGCGCCCCCCAAACGCCCCAGTGCAAGGCTGGa                                                                                             <  8:60198/135‑1 (MQ=255)
                  aaGTAGTACAGGCCGAGGAAGATCTGCAAGAACAGGAACATGAACACGTTGGACGAGAAGACCATGCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGa                                                                                             <  3:209931/135‑1 (MQ=255)
                  aaGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGa                                                                                             <  2:255709/135‑1 (MQ=255)
                  aaGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGa                                                                                             <  8:35126/135‑1 (MQ=255)
                  aaGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGCAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGa                                                                                             <  4:218303/135‑1 (MQ=255)
                           aGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACTAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTa                                                                                   <  8:143183/136‑1 (MQ=255)
                           aGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAACCCCCCCAGTGCAATGCGGGCAGGCAACt                                                                                     >  8:333102/1‑134 (MQ=255)
                           aGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTa                                                                                   <  3:33379/136‑1 (MQ=255)
                           aGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTa                                                                                   <  6:338862/136‑1 (MQ=255)
                             gCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCACAGCGCCCCCCAAACCCCCCCGTGCAAGGCGGGAAGCGAACt                                                                                     >  3:319848/1‑132 (MQ=255)
                             gCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCCGGGCAAGGCGGGGAGGCAACt                                                                                     >  3:230663/1‑132 (MQ=255)
                               cGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAACCCCCCCCGGGCAAGGCTGGAAGGCCAATTAGATc                                                                               >  4:33379/1‑136 (MQ=255)
                                gAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCCAACCCCCCAGTGCAAGGCGGGCAGGCAACGTAGATcc                                                                              >  6:387810/1‑136 (MQ=255)
                                     aGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCtgtg                                                                         >  4:89408/1‑136 (MQ=255)
                                     aGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCtgtg                                                                         <  4:18301/136‑1 (MQ=255)
                                     aGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCtgtg                                                                         <  4:322690/136‑1 (MQ=255)
                                     aGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCtggg                                                                         >  5:12667/1‑134 (MQ=255)
                                          ggCAAGAACAGGAACAGGACCACGTTGGACGAGAAGACCCTCCCGGTTCTCCTAATTTGTTCACAGCCTCCAGGGCAAAGCGCCCCCCCCACCCCCCAGGGCATGGCTGGCAGGGACGGTAGATCCCTGgg‑ggggg                                                                   >  1:406886/1‑133 (MQ=255)
                                          ggCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAACCCCCCGAGTGCAAGGGTGGTTGGGGAGTGAGATCCCGGTG‑GGGGc                                                                   >  1:278323/1‑136 (MQ=255)
                                          ggCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTG‑GGAGc                                                                   <  2:1470/136‑1 (MQ=255)
                                            cAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCC‑GTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAACCCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTG‑GGTGCggg                                                                >  2:1938/1‑136 (MQ=255)
                                              aGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAATCGCCCCCCAAACCCCCCAGTGGAAGGCTGGAAGGCAACTTAGATCCCAGTG‑GGAGCggg                                                                >  6:199286/1‑135 (MQ=255)
                                                   aggaacaggaacACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCACACCCCCCAGTGCAAGGCGGGAAGGCAACTTAGATCCCTGTG‑GGGGCGGGGCAGcc                                                          >  2:293417/1‑136 (MQ=255)
                                                                    ggACGAGACGCCCATCCCGGTGCTCATAATTTGTCCACAGCATCCAGGCCAAAGCGCCCCCCAAACCCCCCAGTGCACGGCTGGAAGGCAACTTAGACCCCTGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTcc                                         <  4:292282/135‑1 (MQ=255)
                                                                            agaCCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTCCGCCCCCGCa                                <  7:323287/136‑1 (MQ=255)
                                                                            accccATCCGGGTTCTCCAAATTTGTCAACCCCATCCAGGGCAAACCGCCCCCCAACCCCCCCCGTGAACGGCTGGACGCCAAATTAGATCCCTGGG‑GGAGGGGGAACCCCCGCCCCCACA‑GGTTCCGCCCCCGCa                                <  2:357298/133‑1 (MQ=255)
                                                                                 aTCCCGGTTCTCATAATTTGTTCCCCCCAGCCCGGCCAAAGCGCCCCCCAACCCCCCCAGGCCACGGCTGGACGGAAACTTAGACCCCTGTG‑GGCGCGGGCAAGCCCGCCCCCACA‑GGTTCCGCCCCCGCAATggg                           <  3:124836/136‑1 (MQ=255)
                                                                                 aTCCCGGTGCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTCCGCCCCCGCAATggg                           <  7:198915/136‑1 (MQ=255)
                                                                                  tCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTCCGCCCCCGCAATgggg                          <  1:293417/136‑1 (MQ=255)
                                                                                   cccGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCACAACCCCCACCGGGCAAGGCTGGACGGCAACCGAGATCCCTGTG‑GGGCCGGGGCAGCCCGCGCCCCCC‑CGGTCCGGCCCGGCCATggggg                         >  6:289453/1‑136 (MQ=255)
                                                                                     cGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGAGCAAGGCTGGAAGGCAACTTAGAACCCTCTGTGGGGGGGGCCAGCCCCGCCCCCCA‑GGTGCGCCCCCCCCATAgggggg                        >  6:273759/1‑135 (MQ=255)
                                                                                       gTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCAGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTCCGCCCCCGCAATGGGGGTCaa                     <  8:255647/136‑1 (MQ=255)
                                                                                       gTTCTCCAAATTTGTTCCCAGCAGCCCGGCCAACCCGCCCCCCAACCCCCCCAGTGCAAGGCGGCACGCCAACTTAGATCCCGGTG‑GGAGCGGGCACCCCCGCTCCCACA‑GGTTCCGCCCCCGCAATGGGGGTCaa                     <  4:230663/136‑1 (MQ=255)
                                                                                       cttCCCCTAATTTGTTCCCAGCACCCAGGGCAAACCGCCCCCCAACCCCCCCAGTGCACCGCTGGACGGCAACTTCGATCCCTGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTCCGCCCCCGCAATGGGGGTCaa                     <  4:256922/135‑1 (MQ=255)
                                                                                          ctcCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCAGTG‑GGAGCCGGGCAGCCCGCACCCCCACGGTACCCCCCCCCCAACggggtccaaa                    >  8:94657/1‑129 (MQ=255)
                                                                                              tAATTTGTTCACAGCACCCCGGGCAAACCGCCCCCCAAACCCCCCAGGGCACGGCGGTAAGGCAACTTAGATCCCTGGG‑GGAGCGGGAAAGCCCGCTCCAACA‑GGTTCCGCCCCCGCAATGGGGGTCAATTCAttt              <  8:60887/136‑1 (MQ=255)
                                                                                                          cccacccAGGCCAACCCCCCCCCAAAACCCCCCAGCCCAAGGCTGGACTGAAATTTATTCCCGTGGG‑GGAGGGGCAAAGCCCGCGCCCACA‑GGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGc  <  1:196523/131‑1 (MQ=255)
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CGATAACGTTGCCCGCTCAAGTAGTACAGGCCGAGGAAGATCGGCAAGAACAGGAACAGGAACACGTTGGACGAGAAGACCATCCCGGTTCTCCTAATTTGTTCACAGCATCCAGGGCAAAGCGCCCCCCAAACCCCCCAGTGCAAGGCTGGAAGGCAACTTAGATCCCTGTG‑GGAGCGGGCAAGCCCGCTCCCACA‑GGTTCCGCCCCCGCAATGGGGGTCAATTCATTTCTTGCTCTCTGC  >  ACB143/1455896‑1456137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑