Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 2,171,262 C→A 11.9% F147L (TTC→TTA)  CJ019_02060 → IS1182 family transposase ISPpu16

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,171,2620CA11.9% 265.5 / 6.4 134F147L (TTC→TTA) CJ019_02060IS1182 family transposase ISPpu16
Reads supporting (aligned to +/- strand):  ref base C (74/42);  new base A (2/16);  total (76/58)
Fisher's exact test for biased strand distribution p-value = 4.02e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GGATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGC  >  ACB143/2171127‑2171393
                                                                                                                                          |                                                                                                                                     
  atctCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCa                                                                                                                                      <  6:67384/132‑1 (MQ=255)
   tttCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCaaa                                                                                                                                    <  7:212678/136‑1 (MQ=255)
                 ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTTCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑cg                                                                                                                    >  3:163363/1‑136 (MQ=255)
                 ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑cg                                                                                                                    >  8:9148/1‑136 (MQ=255)
                 ccGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCAGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGGTCAAA‑GCAGTGCACAAT‑cg                                                                                                                    >  7:339428/1‑136 (MQ=255)
                         cGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGt                                                                                                             >  1:172810/1‑135 (MQ=255)
                            aaGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACt                                                                                                         >  7:198142/1‑136 (MQ=255)
                              ggACAACGGTAAAGCCATTGGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTTAACAAT‑CGCGACCGTAACTTc                                                                                                       <  7:386734/136‑1 (MQ=255)
                              ggAAAACGTTAAAGCCATTCGTAGCGTCTGCCGTCAGTTGGTGATCCTCTGCCTTAAC‑CTCAACCTTTTCTCTTTGTCTATCATTGCC‑ATCGAC‑GGCAGCAATTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTc                                                                                                       <  8:204632/136‑1 (MQ=255)
                                     gttAAAGCCATTCGTCGCGTCTGCCGTCTGTTCGTGAGCCTGTGCCTTAAC‑CTCAACCTTTTCTCTGTGTCCATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAgg                                                                                                <  7:298512/134‑1 (MQ=255)
                                     ggaaaaGCAATTCGTCGCGTCTGCCGTCTGTTCGTGATCCTCTGCCGAAAC‑CTAAACATTTTCTCTGTGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTAAAA‑GCAGTCAAAAAC‑CGCGACCGTAACTTCACCCAgg                                                                                                <  8:1547/133‑1 (MQ=255)
                                     ggTAAAGCCATTCGTAGCGTCTGCCGTCTGTTCTTTACCATATGCCGAAAC‑CTCAACCTTTTCTCTGCTGCGATAATTGCC‑ATCGCC‑GGCAGCACGTTAAAA‑GCTTTTAAAAAT‑CGCGACCGTAACTTCACCCAgg                                                                                                <  2:94490/136‑1 (MQ=255)
                                     ggTAAAGCAATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTCAACAAT‑CGCGACCGTAACTTCACCCAgg                                                                                                <  7:382812/136‑1 (MQ=255)
                                          acccATTCGTCGCGGCTGCCGGCAGTGCGGCAGCCTCTGCCGAACC‑CTCAACCTTTGCTCTGAGGCTATCATGCCC‑ACCGCC‑GGCAGCATTTACAAC‑GCCGTCAAAAAC‑CGCGACCGTAACTTCACCCAGGCCAAg                                                                                           <  8:17820/134‑1 (MQ=255)
                                          aGCCATTCGTCGGGTGTGCCGGCTGTTCGTGATCCTCTGCCTAAAC‑CTCAACATTTTTTCTGAGTCCAACATTCCC‑ATCGAC‑GGCAGAAAGTACAAA‑CCTGTCAAAAAC‑CGCGACCGTAACTTCACCCAGGCCAAg                                                                                           <  2:141026/136‑1 (MQ=255)
                                           gCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTAAAA‑GCATTGAAAAAT‑CGAGACCGTAACTTCACCCAGGCCAAgg                                                                                          >  5:64226/1‑136 (MQ=255)
                                            ccATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                         >  1:287091/1‑136 (MQ=255)
                                             cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTTCC‑ATCGAC‑GGCAGCAAGTACAAA‑CCAGTTAACAAT‑CGCGAACGTAACTACCCCCAGGCCAAggg                                                                                         >  6:127042/1‑134 (MQ=255)
                                             cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGTACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                        >  2:381102/1‑136 (MQ=255)
                                             cATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGt                                                                                         >  4:189149/1‑135 (MQ=255)
                                               ttCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCCGCAAGTTCAAA‑GCAGTGAACAAT‑AGCGACCGTAACTTCACCCAGGCCAAGGTg                                                                                        >  6:61750/1‑134 (MQ=255)
                                                 cGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAgg                                                                                    >  7:308721/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTACC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GAAGTGAACAAT‑CGCGACCGTATCTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  6:361580/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTTCC‑ATCGAC‑GGCAGCAACTTCAAA‑GCAGTGAACAAT‑CGCTACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  7:28842/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  8:371150/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  6:137885/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAAATTCACACAGGCCAAGGTGAAGGc                                                                                   >  6:307106/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAAAAAT‑CGCGACCGTAACTTCACCCAGCCCAAGGTGAAGGc                                                                                   >  6:353421/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAA‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGACAAGGTGAAGGc                                                                                   >  4:139861/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCAGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑AGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGc                                                                                   >  2:67263/1‑136 (MQ=255)
                                                  gTAGCGTCTGCCGTCAGTACGTGATCCTCTGCCGTAAC‑CACAACCTTTTCTCTGAGTCGATCATTGCC‑AACGAC‑GGCCGCAAGTTCACA‑GCAGAGAACAAT‑CGCGACCGTAACTTCCACCACGCCAAGGTGCAGGc                                                                                   >  4:230278/1‑136 (MQ=255)
                                                        tCTGCCGTCTGTTCGTGATCTCCTGCCGAAAC‑CACAACCTTTTCTGTGAGTCTATCATTGCC‑AGCGAC‑GGCAGAAAGTACAAC‑GCCGTCAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCAt                                                                             <  2:172810/136‑1 (MQ=255)
                                                         cTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  3:245150/1‑136 (MQ=255)
                                                         cTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  2:72223/1‑136 (MQ=255)
                                                          tGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  3:369651/1‑135 (MQ=255)
                                                           gCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGCC‑GGCAGCAAGTTCAAA‑GCAGTGAAAAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATg                                                                            >  7:150202/1‑134 (MQ=255)
                                                           gCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGCAAAAT‑CGCGACCGTAACTTCAACCAGGCCAAGGGGCAGGCCCGCATggc                                                                          >  2:208589/1‑134 (MQ=255)
                                                             cGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATgcag                                                                         >  6:397775/1‑135 (MQ=255)
                                                               acAGTTCGTGACCCTCTGCCGAACC‑CACAACATTTTCTCTGAGCCGAAAATTCCC‑ATCGAC‑GGCAGCAAGTGAAAA‑GCAGTCAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCaa                                                                      <  5:62223/135‑1 (MQ=255)
                                                                   ttCGTTATCCTCTGCCTAAAC‑CAAAACTTTTTCTCTGAGTCTATAATTGCC‑AGCGAG‑GGCAGCAAGTAAAAA‑GCTGTCAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                  <  8:82901/136‑1 (MQ=255)
                                                                    tgttGATCCTGTTCCTTAAC‑TTAATNATTTTNTCTGTNTCTTACATTCCA‑TTCGAG‑GGAAGCATTTTAAAA‑CCTTTAAACAAA‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                  <  2:361344/132‑1 (MQ=255)
                                                                    tCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATc                                                                   >  5:396453/1‑134 (MQ=255)
                                                                    gcGTGAGCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGGCGATCATGGCC‑ATCGAC‑GGCAGCAAGTTCAAC‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCg                                                                  <  8:125500/134‑1 (MQ=255)
                                                                     ggTGATCCTCTGCCGTAAC‑CTCAACCTTTTGTTTGTTTCTTTAATTGCC‑TTTGAG‑GTCAGAAATTTAAAA‑CCATTTAAAAAA‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                 <  4:153157/134‑1 (MQ=255)
                                                                     ctttaTCCTGTGCCGTACC‑CTCAACTTTTTCTCTGAGGGGATAATGTCC‑ATCGAC‑GGCATCATGTTCAAA‑GCAGTTAAAAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                 <  8:298047/131‑1 (MQ=255)
                                                                     cGTGATCCTCTGCCGTAAC‑ATCAACCTTTTCTCTGAGTCTATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTTAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCga                                                                 <  4:226778/135‑1 (MQ=255)
                                                                      tttaggctctGACGAAAC‑CTAACCATTTTCTTGTCGTCTATCATTG‑C‑ATCACCGGGCATAAATTTCAAA‑GCTGT‑AACAATCCGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagc                                                               <  4:100778/130‑1 (MQ=255)
                                                                         acccTCTCCCGTAAC‑CACACCGTTTTCTCGGCGCCAAGAATTCCC‑ATCGAC‑GGCAGAATTTAAAAA‑CCATTAAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                            <  3:340583/134‑1 (MQ=255)
                                                                          tCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGCGTCGATCATTTCC‑ATCGCC‑GGCAGCAAGTTAAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgctcagag                                                           >  5:202367/1‑136 (MQ=255)
                                                                          tCCTCTGCC‑TAACACTCAACATTTTCTCTGAGTCTATAATTGCC‑ATCGAG‑GGCAGCAAGTTCAAA‑GCAGTAAAAAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcaga                                                            <  3:230424/135‑1 (MQ=255)
                                                                          accTCTGCCGTCAC‑CACACCATATCATCTGAGCGGATCTTACCC‑ATCCAC‑GGCTCCAAGATCACA‑GCAGTAAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagag                                                           <  2:384008/135‑1 (MQ=255)
                                                                           ccTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGAAAATCgagcagag                                                           >  3:284279/1‑135 (MQ=255)
                                                                           ccTCTCCCTAAAC‑CACAACTTTTACTCTGTGTCCATAATTGCC‑ATCGAG‑GGCAGCAATTTCAAA‑CCAGTAAACAAA‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCgagcagagc                                                          <  4:370516/136‑1 (MQ=255)
                                                                           ccTCGGCGGAAAC‑ATCAACTTTTTCACGGAGTCCATCATTGCC‑ATCGCC‑GGCAGC‑ATTTCAAACCCAGGTAAAAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCGGCATGCAGCAAATCgagcagagc                                                          <  7:182915/136‑1 (MQ=255)
                                                                                gcctaaaC‑CTCAACCTTTTATCTGCGCCCACCATTGCC‑ATCTAG‑GGCAGCATGTTAAAA‑GCTTTCAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  6:333171/131‑1 (MQ=255)
                                                                                gccgacaC‑CTAAACCTTTTCCATCAGGCCATCATTCCC‑ATCCAG‑GGCACCAAGTACAAC‑CATGTCAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  4:79656/130‑1 (MQ=255)
                                                                                gCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATAGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCAtt                                                       >  1:361344/1‑134 (MQ=255)
                                                                                gCCGTAAC‑CTAAACTTTTTCTCTGAGCCGATATTTGCC‑ATGCAG‑GGAAGAATTTTAAAA‑GCATTCAAAAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  3:374438/136‑1 (MQ=255)
                                                                                gCCATACC‑CTCACCCTTTTCTCTCCGTCGAGAATTGCC‑AGCGAG‑GGCAGCATGTTCAAC‑GCAGTCAACACC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  6:360310/136‑1 (MQ=255)
                                                                                cccGTAAC‑CCCAACATTTTCTCTGCGTC‑CTAAATGCCAAGCTCG‑GGCAGAAATTACAAA‑CCAGTCAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  4:201381/135‑1 (MQ=255)
                                                                                 ccttAAC‑CTCAACTTTTTCTCTGAGTCTATCTTTCCC‑ATCGAC‑GCCAGAAATTCAAAA‑CCAGTCAAAAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  4:90643/132‑1 (MQ=255)
                                                                                    tAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCCCCCAGGCCAAGGTGAAGGCCCGCAGGCAGCAAATCGAGCAGAGCATTGAgcgc                                                 >  8:136169/1‑136 (MQ=255)
                                                                                       c‑cTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGCGCGCTAc                                              >  8:144184/1‑136 (MQ=255)
                                                                                       c‑cTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                              >  8:374225/1‑136 (MQ=255)
                                                                                       c‑cTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAc                                              >  3:370020/1‑136 (MQ=255)
                                                                                         cTCAACTTTTTCTCTGAGTCGATCATTCCC‑ATCGCC‑GGCAGCAATTTAAAA‑GCAGTCAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTAcc                                             <  5:116875/136‑1 (MQ=255)
                                                                                          tCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GCCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTAACCCAGGCCAAGGTGAAGGACCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCAAcc                                             >  6:37792/1‑135 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTCCCTTACAg                                       >  1:22893/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  2:390979/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  3:187057/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  3:201240/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  7:62106/1‑136 (MQ=255)
                                                                                               cTTTTCTCTGAGTCGATCATAGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAAAAAT‑CGCGACCGTAACTTCACCCAGGGCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAg                                       >  3:65070/1‑136 (MQ=255)
                                                                                                    ctctGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATg                                  >  5:322579/1‑136 (MQ=255)
                                                                                                     tctGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATAGAGAGATACCTTACAGCGATg                                  >  8:222099/1‑135 (MQ=255)
                                                                                                      ctGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGa                                >  1:353894/1‑136 (MQ=255)
                                                                                                        gatgctaTCATTCCC‑TTCCAG‑GGAAGCATGTACAAA‑GCCTTCAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGAt                               <  1:365592/129‑1 (MQ=255)
                                                                                                         aGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCACTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTc                             >  4:196411/1‑136 (MQ=255)
                                                                                                           tCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  4:115007/1‑136 (MQ=255)
                                                                                                           tCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  4:109155/1‑136 (MQ=255)
                                                                                                            cGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCTACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGAATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  5:206721/1‑135 (MQ=255)
                                                                                                            cGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  7:169418/1‑135 (MQ=255)
                                                                                                            cGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCgg                           >  3:214174/1‑135 (MQ=255)
                                                                                                            cGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGc                          >  2:173300/1‑136 (MQ=255)
                                                                                                                 aTTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                     >  4:315913/1‑136 (MQ=255)
                                                                                                                 aTTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGAATTGAGCGCTACCTTACAGCGATGGATTCGGCAGAcc                     >  5:177696/1‑136 (MQ=255)
                                                                                                                  ttccc‑AGCGAC‑GGCAGCAAGATCAAA‑CCAGGGAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCGAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                    <  5:125315/133‑1 (MQ=255)
                                                                                                                  ttGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     >  6:93790/1‑103 (MQ=255)
                                                                                                                  ttGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGa                                                     <  5:93790/103‑1 (MQ=255)
                                                                                                                   tccc‑ATCGAC‑GGCAGCATGTTCAAA‑GACGTCAAAAAG‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCg                    <  2:238407/133‑1 (MQ=255)
                                                                                                                      c‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGc                 >  7:55805/1‑135 (MQ=255)
                                                                                                                        aTCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCaa               <  8:37452/136‑1 (MQ=255)
                                                                                                                            aC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGcc           >  5:211735/1‑136 (MQ=255)
                                                                                                                            aC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGcc           >  6:168737/1‑136 (MQ=255)
                                                                                                                                gcagcaAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCg          >  6:209519/1‑134 (MQ=255)
                                                                                                                                    caATTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    <  8:261731/136‑1 (MQ=255)
                                                                                                                                    caAGTTCACA‑GCAGTCACAAAA‑CGCGACCGTAACTTCACCCAGGCCAAGGTTAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    <  8:146538/136‑1 (MQ=255)
                                                                                                                                    caAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    >  7:322900/1‑136 (MQ=255)
                                                                                                                                     aaGTTAAAA‑CCAGCCAACAAC‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTg    <  5:148875/135‑1 (MQ=255)
                                                                                                                                       gTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGc  >  8:275206/1‑135 (MQ=255)
                                                                                                                                          |                                                                                                                                     
GGATTTCAAAACCATCGCCGACTTTCGCAAGGACAACGGTAAAGCCATTCGTAGCGTCTGCCGTCAGTTCGTGATCCTCTGCCGTAAC‑CTCAACCTTTTCTCTGAGTCGATCATTGCC‑ATCGAC‑GGCAGCAAGTTCAAA‑GCAGTGAACAAT‑CGCGACCGTAACTTCACCCAGGCCAAGGTGAAGGCCCGCATGCAGCAAATCGAGCAGAGCATTGAGCGCTACCTTACAGCGATGGATTCGGCAGACCGGGCAATGCCGGAGGTGGC  >  ACB143/2171127‑2171393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑