Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	3,130,824	(T)9→8	intergenic (+212/‑78)	livF_3 → / → oprD_7	High‑affinity branched‑chain amino acidtransport ATP‑binding protein LivF/Porin D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,130,816	0	T	.	100.0%	206.7 / NA	44	intergenic (+204/‑86)	livF_3/oprD_7	High‑affinity branched‑chain amino acidtransport ATP‑binding protein LivF/Porin D
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (23/21);  total (23/21)

AACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGC  >  ACB143/3130683‑3130942                                                                                                                                      |                                                                                                                               aaCGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGttt                                                                                                                              >  3:171495/1‑136 (MQ=255) aaCGCGGCCCAGGCCGCGGTTTTTTGGCCCTGACGAAAGCGGTGTGGGGCCGGGAACAGCAGTTGTTCATATTGCTTAGCAGACGGAGCATGATAGTGTTGAGGAAGTTTTTCAATTGGTTGTATAACAATGGttt                                                                                                                              >  8:105761/1‑136 (MQ=255)                    ttttttAGCCCTGACGAAAGCGCTGTGGCGCCGGGAACAGCAGGTGTGCAGATTGCTTAGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TGTTTTTTGTTGGCATGgttgt                                                                                                          >  8:103039/1‑136 (MQ=255)                           gCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGaac                                                                                                    <  4:6791/135‑1 (MQ=255)                                              ggCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTg                                                                                >  3:370878/1‑136 (MQ=255)                                              ggCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTg                                                                                >  1:237652/1‑136 (MQ=255)                                               gcgcCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTgg                                                                               <  6:262706/136‑1 (MQ=255)                                                     gCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTAGTTCAATTCCTTGAATAACAATGG‑TATTTTTTCATGGCATGGTTGTTGGAACAAAGGGATCTTCAGCGCCTGGAAAGgc                                                                         >  3:323553/1‑136 (MQ=255)                                                             agTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCt                                                                 <  6:87380/136‑1 (MQ=255)                                                                    cAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCAtt                                                          <  6:312836/136‑1 (MQ=255)                                                                       aTTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTcc                                                        >  7:136685/1‑135 (MQ=255)                                                                          gcctCGCAGAAGGAGCATCATAGTGTTGATGAATTTGTTCAATTGCTTGAATAAAAATTG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAg                                                     <  6:3294/132‑1 (MQ=255)                                                                          gCTTCGCAGACGGAGCATCATAGTGTTGAGGTAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAg                                                     <  4:226079/135‑1 (MQ=255)                                                                           cTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGa                                                   <  5:178785/136‑1 (MQ=255)                                                                                cAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACt                                              <  2:237652/136‑1 (MQ=255)                                                                                   aCGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGcc                                           <  3:314453/136‑1 (MQ=255)                                                                                          atcatAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATTG‑TTTTTTTTGTGGTCATGGTTGTTTGAACAACGTGATCTGCCTCGACTGGAAATGAGCCTCACTCGTAAGTCCAAGTATCACTGCAATgcgc                                     >  4:51088/1‑135 (MQ=255)                                                                                          atcatAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGct                                    <  2:64356/136‑1 (MQ=255)                                                                                          atcatAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGct                                    <  5:84764/136‑1 (MQ=255)                                                                                          atcatAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGct                                    <  7:105761/136‑1 (MQ=255)                                                                                                gtgtTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTAcc                               <  8:106593/135‑1 (MQ=255)                                                                                                gtgtTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTAcc                               <  5:158463/135‑1 (MQ=255)                                                                                                  gtTGAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGtt                            >  6:39341/1‑136 (MQ=255)                                                                                                     gAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTg                          <  4:318256/135‑1 (MQ=255)                                                                                                     gAGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTg                          <  4:170629/135‑1 (MQ=255)                                                                                                      aGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGc                                            <  1:133289/116‑1 (MQ=255)                                                                                                      aGGAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGc                                            >  2:133289/1‑116 (MQ=255)                                                                                                        gAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTa                       >  4:277275/1‑135 (MQ=255)                                                                                                        gAAGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGATCTACCTTTTGCTa                       >  2:97794/1‑135 (MQ=255)                                                                                                        gAAGTTGGTCAATTGCTTGAATAACACTGG‑TTTTTTTTGTTGGCATGGGTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTAc                      <  6:177287/136‑1 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGCTCTGCAGCGCATGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCAATGCGCTCTACAGTTTGATAcc                     >  8:246436/1‑135 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTa                       >  4:42280/1‑134 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTa                       >  4:267191/1‑134 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTa                       >  3:346206/1‑134 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTa                       >  2:101811/1‑134 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTa                       >  8:161230/1‑134 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTa                       >  8:211418/1‑134 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACa                     >  7:119054/1‑136 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACa                     <  1:20844/136‑1 (MQ=255)                                                                                                         aaGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCAATGCGCTCTACCGTTTGCTa                       >  8:219566/1‑134 (MQ=255)                                                                                                          aGTTGTTCAATTGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGTGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAg                    <  3:88839/136‑1 (MQ=255)                                                                                                                     tGCTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCttt         >  2:120282/1‑136 (MQ=255)                                                                                                                       cTTGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTtc        <  1:220604/135‑1 (MQ=255)                                                                                                                         tGAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTctct     >  5:65910/1‑136 (MQ=255)                                                                                                                          gAATAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGACTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTg    >  6:6931/1‑136 (MQ=255)                                                                                                                            aTAACAATGG‑TTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGc  >  3:231824/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                               AACGCGGCCCAGGCCGCGTTTTTTTCGCCCTGACGAAAGCGCTGTGGCGCCGGCAACAGCAGTTGTGCAGATTGCTTCGCAGACGGAGCATCATAGTGTTGAGGAAGTTGTTCAATTGCTTGAATAACAATGGTTTTTTTTTGTTGGCATGGTTGTTGGAACAACGGGATCTGCAGCGCCTGGAAAGGCGCCTACCTCGTCATTCCAAGGATCACTGCCATGCGCTCTACCGTTTGCTACAGAACAGCTCTTTCTCTGGC  >  ACB143/3130683‑3130942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑