| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 3,804,739 | (C)6→7 | coding (23/1185 nt) | ydhP_3 → | Inner membrane transport protein YdhP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 3,804,733 | 1 | . | C | 93.0% | 151.7 / 4.0 | 43 | coding (17/1185 nt) | ydhP_3 | Inner membrane transport protein YdhP |
| Reads supporting (aligned to +/- strand): ref base . (2/1); new base C (24/16); total (26/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGACACGGGCTGCAACGTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACA‑C‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTT > ACB143/3804606‑3804862 | aGGACACGGGCTGCAACGTCGCGGAAGCCGGGGCAGCATCCTCGCCGTTGCCGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATATGGATACAGATGTCAGTCCAGGGACA‑c‑ccccccg > 2:251418/1‑134 (MQ=255)aGGACACGGGCTGCAACGTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATACCGCCGTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGCACA‑c‑ccccccg > 6:82351/1‑134 (MQ=255)aGGACACGGGCTGCAACGTCGCGGAAGCCGGGGAAGCATCCTCGACGTCGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACA‑c‑ccccccg > 6:368487/1‑134 (MQ=255)aGGACACGGGCTGCAACCTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCGTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGACcc‑c‑ccccccg > 3:218186/1‑134 (MQ=255) gACACGGGCTGCAACCTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGAAGTCAGTCCAGGTACA‑c‑ccccccgct > 7:243650/1‑132 (MQ=255) acacGGGCTGCAACGTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACA‑c‑ccccccgctc < 2:3562/136‑6 (MQ=255) gCTGCAACTTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGt < 2:365437/136‑1 (MQ=255) cTGCAACGTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGTCTATTATCCCGCCTTGCACGTTTCCGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGt > 3:224061/1‑135 (MQ=255) cTGCAACCTCCCGGAAGCCGGGGGAGCATCCTCGACGTTGCAGGCATCGATTGACTATTATCCCGCCGTGAACGGTTGAGGATTTTTCAATAGGGATACAGATGTGAGTCCAGGAACACC‑CCCCCGCTCCCCAGt > 8:297299/1‑135 (MQ=255) gCAACGTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTaa > 1:231846/1‑135 (MQ=255) gCAACGTCGCGGAAGCCGGGGCAGCATCCTCGACGGTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCGCCCCACTaa > 2:235554/1‑135 (MQ=255) aaGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGc > 5:17079/1‑135 (MQ=255) gggCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATg > 5:231554/1‑136 (MQ=255) ggCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATg > 7:178226/1‑135 (MQ=255) gcagcaTCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGa < 2:77537/136‑1 (MQ=255) cagcaTCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGcc > 3:168823/1‑127 (MQ=255) agcaTCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGcc > 6:17923/1‑126 (MQ=255) aTCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAgccg < 1:24941/136‑1 (MQ=255) aTCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAgccg < 6:309428/136‑1 (MQ=255) aTCCTCGACGTTGCAGCAATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAgccg < 6:7293/136‑1 (MQ=255) ccTCGACGTTGCAGCCATCGATGGACTATTATCCCGCCTTGAACGTTTCAGCATGGTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAACCACCCTCGCCAAGATGGAAgccgc > 4:174336/1‑135 (MQ=255) aGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTggg > 3:79414/1‑135 (MQ=255) gATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACA‑C‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCACCATGGCACTGGGCAGCTTTGc < 3:169136/136‑1 (MQ=255) aCTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTAGCCATc > 2:311951/1‑136 (MQ=255) cccGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCgg > 4:242055/1‑136 (MQ=255) cTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAAt < 2:84060/136‑1 (MQ=255) cTTGAACGATTCAGCATGTTTCAATAGCGATACAGCTGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCGATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAAt < 1:255824/136‑1 (MQ=255) tGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTc > 7:278217/1‑136 (MQ=255) tGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACA‑CACCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCCCTGGGCAGCTTTGCCATCGGCACCGGCGAATTc > 1:74085/1‑136 (MQ=255) tGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACA‑C‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCg > 1:235764/1‑136 (MQ=255) gAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAAtt < 3:181076/134‑1 (MQ=255) aaCGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCCCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGc > 1:144303/1‑136 (MQ=255) cGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCca < 5:252380/136‑1 (MQ=255) tttCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCcatc < 2:128754/136‑1 (MQ=255) aGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGATTTCGCAATCATgg > 3:240173/1‑136 (MQ=255) aGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATgg > 5:6972/1‑136 (MQ=255) aGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCAGCATgg > 5:11302/1‑136 (MQ=255) tttCAATAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGa < 8:161644/136‑1 (MQ=255) aaTAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCCCCGGCGAATTCGCCATCATGGGCCTGAGGcg > 4:4679/1‑135 (MQ=255) aaTAGGGATACAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGcc > 3:370832/1‑136 (MQ=255) cAGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGc < 4:79414/135‑1 (MQ=255) aGATGTCAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCa < 3:374071/136‑1 (MQ=255) cAGTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGTAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAAt < 3:308966/135‑1 (MQ=255) gTCCAGGAACACC‑CCCCCGCTCCCCAGTAGAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGCCATTGCCAGCAATTTg > 1:358033/1‑136 (MQ=255) gTCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTg > 7:344647/1‑136 (MQ=255) tCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAgccg < 5:342783/55‑1 (MQ=255) tCCAGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAgccg > 6:342783/1‑55 (MQ=255) aGGAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGt > 5:123387/1‑136 (MQ=255) ggAACACC‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGtt < 8:264660/136‑1 (MQ=255) | AGGACACGGGCTGCAACGTCGCGGAAGCCGGGGCAGCATCCTCGACGTTGCAGCCATCGATTGACTATTATCCCGCCTTGAACGTTTCAGCATGTTTCAATAGGGATACAGATGTCAGTCCAGGAACA‑C‑CCCCCGCTCCCCAGTAAAAACACCGTCGCCAAGATGGAAGCCGCCATGGCACTGGGCAGCTTTGCCATCGGCACCGGCGAATTCGCCATCATGGGCCTGATGCCCGACATTGCCAGCAATTTGCAGTT > ACB143/3804606‑3804862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |