Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,626,0890TG40.0% 23.9 / 10.7 35V174G (GTG→GGG) hcf136_3Ycf48‑like protein
Reads supporting (aligned to +/- strand):  ref base T (11/10);  new base G (0/14);  total (11/24)
Fisher's exact test for biased strand distribution p-value = 8.97e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGC  >  ACB143/3625955‑3626219
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ggcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTGGGTGCCTACGGGGTgg                                                                                                                                   >  2:139322/1‑137 (MQ=255)
 gcagCGGAGGCGGCCGCAGACGAGCGGCGCGTGGCGGGGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGc                                                                                                                                  <  4:231505/137‑1 (MQ=255)
 gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGc                                                                                                                                  >  4:218453/1‑137 (MQ=255)
      cGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑gc                                                                                                                            >  3:79513/1‑137 (MQ=255)
           cTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGACAT‑GCGCAcc                                                                                                                       >  3:27894/1‑137 (MQ=255)
                 cAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCGACGGGGTGGCCAT‑GCGCACCgccgac                                                                                                                 >  4:108726/1‑137 (MQ=255)
                  aGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGccgacg                                                                                                                >  5:211038/1‑137 (MQ=255)
                      gAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGACGAcggcgg                                                                                                            >  7:241385/1‑137 (MQ=255)
                                                gctggtGGCCGACGGCGCGGACAAGCCGTTGTGGCCCCTCAATTTCACCGATCCCCCCACGGGCGTGGTCGGCGGTGCCTAGGGGGTGGCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGcc                                                                                  <  5:204660/137‑1 (MQ=255)
                                                  ggctgGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGGGCCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGa                                                                                <  8:191162/136‑1 (MQ=255)
                                                       gccgccGGCGCGGACAAGCGGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGGCGGCGGGGCCTAGGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  8:138958/137‑1 (MQ=255)
                                                       gCCGACGGCGCGGACAAGCGGTTGTGGGCCCTGAATTTCCCCGATGCCCGCACCGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAG‑GCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  6:209532/137‑1 (MQ=255)
                                                       gCCGACGGCGCGGACAAGCGGTTGTGGGCCCTGAATTCCACCGATGCCCGCACCGGCCGGGGCGTCGGTGCCTACGGGGGGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  4:23508/137‑1 (MQ=255)
                                                       gCCGACGGCGCGGACAAGCCGTTGTGGGCCCTGATTTTCCCCGATCCCCGCACCGGCCGGGGCGGCGTGGCCTACGGGGTGGCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  7:173734/137‑1 (MQ=255)
                                                             ggggcgcACACGCGGTTGTGGGCCCTGAATTACCCCGAGGCCCGCACGGGCGTGGGGGTGGGGGCCT‑CGGGGGGGGCATGGCCCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg                                                                     <  2:265477/134‑1 (MQ=255)
                                                             ggCGCGGACAAGCCGTTGTTGGCCCTTAATTTCACCGATGCCCGCAACGGCCTGGGCGGCGGTGCCTCCGGGGGGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg                                                                     <  5:155177/137‑1 (MQ=255)
                                                             ggCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCACCGGCCTGGTGGGCGGGGCCTCCGGGGTGGCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg                                                                     <  2:211020/137‑1 (MQ=255)
                                                               ggcgGACATGCGGTTGTTGGCCCTGAATTCCCCCGAGCCCCCCACCGGCGTGGGCGGCGGGGCCGCGGGGGGGCCCAT‑GCCCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGc                                                                   <  6:174519/136‑1 (MQ=255)
                                                                  ggAAAAGCCGTTGTTGGCCCTGAATTTCCCCCAGCCCCGCAACGGCCTGGGCGGCGGTGCCTACGGGGGGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTg                                                                <  8:7758/137‑1 (MQ=255)
                                                                    aCAAGCGGTTGTTGGCCTTCAATTTCCCCTCTCCCCGCAACGGCCTGGTCGGCGGTGCCTAGGGGGGGGCCTT‑GCGCCCGGAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                              <  1:268651/137‑1 (MQ=255)
                                                                    aCAAGCGGTTGTGGGCCCTGAATTTCACCGATCCCCGCACCGGCCTGGGCGGGGGTGCCTCGGGGGGGGCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                              <  3:218453/137‑1 (MQ=255)
                                                                    aCAAGCCTTTGTTGGCCCTGAATTGCACCCTTCCCCGCCAGGGCCGGGGCGGCGGTGCCTACGGGGGGCCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                              <  8:124135/137‑1 (MQ=255)
                                                                    aCAAGCCGTTGTTGGCCCTGAATTTCACCGAGGCCCGCACCGGCCTGGTGGTCGGTGCCTAGGGGGGGGCCTT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                              <  3:254597/137‑1 (MQ=255)
                                                                     aacgcggttgttGGCCCTTAATTTCCCCGATGCCGGCAAGGGCCGGGGCGTGGGGGCCTCCGGGGGGGCCTT‑GCGCAGCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGAc                                                             <  3:35833/131‑1 (MQ=255)
                                                                                         aTTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGgcg                                         >  3:22879/1‑137 (MQ=255)
                                                                                                ggttGCCCGCAACGGCGGGGGCGTGGGTGCTTACGGGGGGGCCAT‑GCGCACCTACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAg                                  <  4:141405/134‑1 (MQ=255)
                                                                                                cccggCCCGCACCGGCCTGGGGGGCGGTGCCGCCGGGGTGGCCAT‑CCCCACCGAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAg                                  <  6:169201/133‑1 (MQ=255)
                                                                                                  atcccccCAACGGCCGGGGCGGCGGGGCCTCCGGGGGGCCCAT‑CCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                                <  3:26031/134‑1 (MQ=255)
                                                                                                   tccccGCACCGGCGGGGTCGTGGGTGCCTCGGGGGGGGCCAG‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCa                               <  1:112050/135‑1 (MQ=255)
                                                                                                     cccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGCCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                             >  1:131034/1‑137 (MQ=255)
                                                                                                          aaCGGCCTGGTCGTCGGGGCCTACGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGgt                        <  8:241385/137‑1 (MQ=255)
                                                                                                                  gggggtcgGGGCCTACGGGGTGGCCAT‑GCGCACCGAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCg                <  7:146844/133‑1 (MQ=255)
                                                                                                                  ggTCGTCGGTGCCTCCGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCg                <  2:43729/137‑1 (MQ=255)
                                                                                                                                aCGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGc  >  5:241215/1‑137 (MQ=255)
                                                                                                                                aCGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGc  >  8:15094/1‑137 (MQ=255)
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GGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCAT‑GCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGC  >  ACB143/3625955‑3626219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑