Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	5,693,153	T→G	36.5%	R228R (CGT→CGG)	pyrC' →	Dihydroorotase‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,693,153	0	T	G	36.5%	32.4 / 8.2	52	R228R (CGT→CGG)	pyrC'	Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (14/18);  new base G (10/10);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC  >  ACB143/5693018‑5693288                                                                                                                                         |                                                                                                                                        ccGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGgcgtgcgt                                                                                                                                         <  7:345244/136‑1 (MQ=255)       ccTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAACCCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTgcgccc                                                                                                                                   >  2:274071/1‑134 (MQ=255)        cTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACGTGCTGC‑TGGTGGAACAGAGCGGcgtgggtggggccg                                                                                                                                  >  3:363738/1‑130 (MQ=255)             ccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGGGTGGGCCCGTCAGc                                                                                                                             >  5:211661/1‑136 (MQ=255)             ccAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGGGTGCGCCCTTCAGc                                                                                                                             >  4:352222/1‑136 (MQ=255)                    ggcCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCCCGTCAGCCAGCTCa                                                                                                                      >  2:42936/1‑136 (MQ=255)                        tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGGGTGCGGACTTAAGCCAGAtaccccg                                                                                                                  >  8:248880/1‑130 (MQ=255)                        tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACATCCGCCACATACCCAg                                                                                                                  >  3:157338/1‑136 (MQ=255)                             cACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCCCGTCAGCCCGATAACCAGCGccc                                                                                                             >  5:319702/1‑136 (MQ=255)                                  aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCCCGTCAGCCAGATCACCAGCGCCCGTGgc                                                                                                        >  1:234859/1‑136 (MQ=255)                                   ggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCCCTTCAGCCCGATCACCACCGCCCGTGgcg                                                                                                       >  7:71718/1‑136 (MQ=255)                                       ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCACCCTGCTGC‑TGGTGGAACAGAGCGGCGGGGGGGGGCCCTTCCGCCCCGTCACCCGCCCCCGGGGcgcgcg                                                                                                   >  4:85623/1‑136 (MQ=255)                                       ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGGGTGCGCAGATCAGCCAGATAAACCCCGCCCGGGGcgcgcg                                                                                                   >  6:95679/1‑136 (MQ=255)                                       ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGcgcgcg                                                                                                   >  8:118491/1‑136 (MQ=255)                                        cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCACATAAACCCGAAAAACACCGCCCGTGgcgcgccg                                                                                                  >  4:178705/1‑134 (MQ=255)                                        cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGAGGCGTGCGGGCGCGCATGAGACAGATCAACCGCCCCCGTGgcgcccca                                                                                                  >  2:77925/1‑132 (MQ=255)                                              cAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGC‑TGGTGGACCAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATc                                                                                            <  8:128220/136‑1 (MQ=255)                                              cAGCTTCCTCGGCCGGCCGGGCATCCCGGAGAGCGCCTAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGCGCGGCGTGCGGGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                            <  3:247480/136‑1 (MQ=255)                                               aGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGATCAGAGCGGCGGGCGGGGGCCCGTCAGCCAGCTGCCCAGCCCCCGGGGCGCGCGGcggctag                                                                                           >  5:318228/1‑130 (MQ=255)                                                    ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGGGGCCTTCAGCCCGATCACCCCCGCCCGTGGCGCGCGGCGGCTCgccccg                                                                                      >  7:58435/1‑134 (MQ=255)                                                     ctgggCGTGCCGGGCAGCCCGGAGCGCGCCTAAACCGGGGCCCTGGCGCGAAACCTGCTGC‑TGGGGTAACCGCGCGGCGGGCGGGCGCACTTCCGCCATACCCCCCGCGCCCGGGGCGCGCGGCTGATCgcccagg                                                                                     <  8:372233/133‑1 (MQ=255)                                                     cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGAACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                     <  5:196037/136‑1 (MQ=255)                                                     cTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                     <  2:125293/136‑1 (MQ=255)                                                         gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGGGCCCTTCAGCCCGCTCACCCGCGCCCGTGGCGCGCGGCGGATCgcccaggcc                                                                                   >  1:48431/1‑134 (MQ=255)                                                         gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACATCAGCCAGATCACCCCCCCCCGTGGCGCGCGGCTGCTAgccccggcccc                                                                                 >  6:303693/1‑135 (MQ=255)                                                         gCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACATCAGGCACAGAACCCACCCCCGGGGGGCGCGGGGGCTCgcccacgcgca                                                                                 >  2:207625/1‑136 (MQ=255)                                                                gggCATCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTGCTGC‑GGGTGGACCAGAGCGGCGTGCGGGCGCACTTCAGCCAGCTCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                          <  5:382688/136‑1 (MQ=255)                                                                gggCAGCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGAGCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                          <  1:339505/136‑1 (MQ=255)                                                                              gcgcCTAAACGGGGGCCCGGGCGCGCAACCTGCTGC‑TGGTGGAACCGCGGGGGGGGGGTGCGCAATTCACCCACATCCCCCCCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  7:154438/136‑1 (MQ=255)                                                                              gcgcCTAAACCGTGGCCCGGGCGCGCAACCTGCTGG‑TGGGGCAACAGCGCGGGGGGCGGGCCCACTTCAGCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  6:211661/136‑1 (MQ=255)                                                                              gcgcCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                            <  7:82271/136‑1 (MQ=255)                                                                               ggcCGAACCCGTGGCCCTGGCGCGCAACCTGCGGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCATTTCAGCCAGAGCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAcc                                                           <  7:247526/135‑1 (MQ=255)                                                                                   gaaccggTGGCCCGGGCGCGCACCCGGCTGG‑TGGTGGACCCGCGCGGGGGGCGTGCGCACTGCCCCCCGACCCCCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                       <  1:206840/130‑1 (MQ=255)                                                                                   gAAACGGTGGCCCGGGCGCGCAACCTGCTGC‑GGGTGGAACAGCGCGGCGTGCGGGCGCACTTCAGCCATATCACCAGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                       <  7:206598/136‑1 (MQ=255)                                                                                   gAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                       <  5:183900/136‑1 (MQ=255)                                                                                   gAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                       <  7:118491/136‑1 (MQ=255)                                                                                     aaCCGTGGCCCTGGCGCGAAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGa                                                      <  2:334858/135‑1 (MQ=255)                                                                                       ccGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGGGGCCCTAAGCCCGCAAACCCACCCCCCGGGCGCGCCGCCGGTCGCCCCCGCCCCCGCCGTGGGCCGGCCGGGGAcccccgcggg                                                   >  2:167595/1‑132 (MQ=255)                                                                                        ggTGGCCCTGGCGCGCAACCTGCTGC‑TGGTTTACCATAGGGGCGGGGGTGCGCACTTCACCCATATACCCCCCCCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  6:389901/135‑1 (MQ=255)                                                                                        cggggCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  8:317746/133‑1 (MQ=255)                                                                                        cgggccccGGGCGCGCAACCGGCGGC‑TGGGGCAACCTCCCGGGGGGCGGGCGCACTTCAGCCCGACCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  2:396533/131‑1 (MQ=255)                                                                                        cGTGGCCCTGGCGCGCAACGTGCTGC‑GGGTGGAACTGAGCGGCGGGCGTGCGCACTTCAGCCATATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  4:304723/136‑1 (MQ=255)                                                                                        cGTGGCCCTGGCGCGCAACCTGCTGC‑TGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  7:65981/136‑1 (MQ=255)                                                                                        cGTGGCCCTGGCGCGAAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                  <  3:93616/136‑1 (MQ=255)                                                                                             cccTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACGTCAGCCACATAACCACCCCCCGTTGCGCCCCGCGGCACGCCCCCGCCCACGCCGTGGGGCTGCCGGTAAACCCCGATTTAtcg                                               >  8:287091/1‑134 (MQ=255)                                                                                                 ggCGCGAAACCGCCTGC‑TGGTGCAACCTCGCGGGGTGCTTGCGAACTCCTCCCCTATCACCCCCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTAc                                         <  8:324670/136‑1 (MQ=255)                                                                                                  gcgcgcAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCCGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCTGTAcc                                        >  1:256237/1‑136 (MQ=255)                                                                                                     cgaacccTGCTGCGTGGT‑GAACATAGCGGCTTGCGGGCGCACTTCACCCAGACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc                                     <  4:303250/131‑1 (MQ=255)                                                                                                       cAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTg                                   <  1:351070/136‑1 (MQ=255)                                                                                                         aCCTGGTGG‑GGGTGCAACAGCGGGGGGTGCGGGCGCATTCCAGCCAGAGCCCCCGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                 <  4:290884/136‑1 (MQ=255)                                                                                                                               gcggggGGCGGGCGCACTCCCGCCCCACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg            <  6:2826/136‑1 (MQ=255)                                                                                                                                cggggTGCGTGCCCACTTCCCCCACATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc           <  5:14696/136‑1 (MQ=255)                                                                                                                                 ggcgTGCGTGCGCACTTCAGCCAGATCACCCCCGCCCCTGGCGCGCGGCTGATCGCCCCGGCCCCCGCCGTGGGCCTGCCCGGAACCGCCGCTGTAGCGCTGTACCCCCTGATCCTCACGGGAGAGTCGCTGcgcg          >  8:60286/1‑136 (MQ=255)                                                                                                                                 ggcgTGCGGGCGCACTTCCGCCCGCTCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCCCGCCCCGGCCTTGGGCCTGCCGGTAACCGCCCCTGTAGCGCTGTACCAGCTGCTACTCACGGCCGCGTCgcggcggg          >  6:190152/1‑134 (MQ=255)                                                                                                                                   cgtgcgtgCGCCCGTAAGCCAGATCACCCGCCCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCCGGAACCGCCGATGTAGCGCGGTACCAGCTGATACGCACGGGCGCGTCGCGGcgcgcg        >  4:261488/1‑134 (MQ=255)                                                                                                                                    gtgcgtgcGCACTGCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  8:83605/136‑1 (MQ=255)                                                                                                                                    gtgcgggcGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  6:130176/136‑1 (MQ=255)                                                                                                                                    gggggtgcgCAGTTCACCATAACCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  2:357631/132‑1 (MQ=255)                                                                                                                                    gggggtgcgAAGTTCCGCCAGATCACCCGCGCCGGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  6:319702/132‑1 (MQ=255)                                                                                                                                    gggcgtgcgCACTTCCCCCATAACCCCCGGGCCGGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  3:92051/134‑1 (MQ=255)                                                                                                                                    gggcgggcgCACTTCCGCCAGACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt       <  3:177453/134‑1 (MQ=255)                                                                                                                                      gcgtgcgCACTTAAGCCCGATCACCAGCCCCCGTGGCGCGCGGCTGATAGCCCAGGCCCAGGCCTTGGGCCTGCCGGGAACCGCCGATGTAGCGCGGTACCACCAGCTCCTCCCGGCCGAGTCGCTGCGCGAGGtc     >  6:297875/1‑136 (MQ=255)                                                                                                                                         tgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc  <  7:110464/136‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CCGCGACCTGGCCCAGGGCGGCCTGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGC‑TGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCC  >  ACB143/5693018‑5693288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑