Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	2,094,790	A→C	21.4%	V192G (GTG→GGG)	CJ019_01995 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,094,790	0	A	C	21.4%	150.0 / 6.1	78	V192G (GTG→GGG)	CJ019_01995	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/19);  new base C (2/17);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 1.51e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATTCGTTGTGCTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATG  >  ACB143/2094658‑2094914                                                                                                                                       |                                                                                                                              aaTTCGTTGTGCTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCAcg                                                                                                                             <  5:141372/135‑1 (MQ=255) aaTTCGTTGTGCTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCAcg                                                                                                                             <  3:216799/135‑1 (MQ=255)      gTTGTGCTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCgcgc                                                                                                                       <  5:101511/136‑1 (MQ=255)      gTTGTGCTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCgcgc                                                                                                                       <  3:47693/136‑1 (MQ=255)      gTTGTGCTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCgcgc                                                                                                                       <  3:376887/136‑1 (MQ=255)           gCTCGGCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGaa                                                                                                                  <  7:221306/136‑1 (MQ=255)            cTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTAGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAg                                                                                                                 >  2:209676/1‑136 (MQ=255)               gTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGcc                                                                                                              <  5:247786/136‑1 (MQ=255)               gTCTTCTGCCGGGGCCATGAGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACTT‑GAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGcc                                                                                                              <  4:64924/136‑1 (MQ=255)                       ccGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGCAGGCCCGgcgcgc                                                                                                      >  1:272004/1‑136 (MQ=255)                         ggggCCATGCGGTCCAGTACGTGGCATTTGGCCCGGGCAAACTTTTACTAAATATACGT‑CAGATGCAATTAATACACCTTCGGCTCGGGGTGCTTGCCAAAATA‑CTCCACGCCGCGCTCGACGGCCAGGCGCGCCt                                                                                                    <  4:329673/136‑1 (MQ=255)                             ccATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCCCGCCGGG‑cc                                                                                               >  6:194459/1‑136 (MQ=255)                              cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTTCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑ccc                                                                                              >  3:307623/1‑135 (MQ=255)                              cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCg                                                                                              >  8:105071/1‑136 (MQ=255)                               agggggTCCAGAACCTGGGCATTGGCCGGGGCCAACAGTTTCTCAAGATACGT‑CCGCTGCTCTTCAAACCCCTTCGGCGCCGGCTGCTTGCGCAAATA‑CTCCCCGCCGCGCTCGACGGCCCGGCGCGCCTGG‑CCgg                                                                                             <  6:69377/132‑1 (MQ=255)                                 ccGGTCCAGTCCCTGGGCATTGCCCCGGGCCAACAGTTGCTCAACATCCTC‑CTGCTGCCATTCATACACCTTCGGCGCCGGCTGCTTGCGAAAATA‑CTCCCCGCCGCGCTCGATGGCCAGGCGCGCCTGG‑CCGGGc                                                                                           <  4:141158/135‑1 (MQ=255)                                    gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTATTCATACACCTTCGGCTCCGGCTCCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACCCGCCCCAGG‑Ccgggcggg                                                                                        >  3:371795/1‑136 (MQ=255)                                        aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGccgt                                                                                    >  4:266861/1‑134 (MQ=255)                                         gTAGCTGGGCATTGGCCGGGCCCATCTTTTTCTAATGTTAGGT‑GCGCGCTTTTTAATACACCTTGGGCGGCGGGGGTTGGCTAAATTA‑CTCCCCCCGGCGCTCGAAGGCCCGCCGCGCCTGG‑CCGGGCTGGCCttt                                                                                   <  4:113837/136‑1 (MQ=255)                                          tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCCGGCTGGCCtttt                                                                                  >  5:321475/1‑136 (MQ=255)                                          tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCCGGCAGGGCtgtt                                                                                  >  2:351170/1‑136 (MQ=255)                                          tACCTGGGCATTGCCCCGGCCCAACTGTTTCTAAACATCCGT‑CCGCTGCGCTTCATCCACCTTCGGCTCCGGCTGCTGGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCtttt                                                                                  <  6:298531/136‑1 (MQ=255)                                            cgtGGGCATGGGCCGGGCCAAACTGTTTCTAAAGATACGT‑CAGCTGCTCTTCAAACACCTGGGGCGCGGGCTGCTTGCGAAAATC‑CGGCCCGCCGCGCGCGACGGCCCGGCGCGCGGGG‑CCGGGCTGGCCTTTTTg                                                                                <  4:124202/134‑1 (MQ=255)                                             cggggCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCGGCTCTTCATACACCTGCGGCTCCGGCTGCTTGCGCAAATC‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGc                                                                               <  8:295087/134‑1 (MQ=255)                                                 gCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGGCCCGGGGGGGCGTTTTTgaggg                                                                            >  5:316203/1‑132 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCAGGCGCCCCGGG‑GCGGGCGGGCCAGTTTGGAGGGc                                                                          >  7:78422/1‑136 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCGGGGCGTTTTTGAgggg                                                                          >  3:164545/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCGGGGCGTTTTGCAgggg                                                                          >  6:147618/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGGGGGGCGTTTTTAAGGGc                                                                          >  5:141613/1‑136 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGG‑CCGGGCGGGGCGTTTTGCAgggg                                                                          >  4:307786/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAgggg                                                                          >  2:326633/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCGGGGCGTTTTTCAgggg                                                                          >  2:26525/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑GCCGGCGGGGCGGTTTGGAgggg                                                                          >  1:313742/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCGTTTTGGAgggg                                                                          >  5:90944/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACGCGCCCCAGG‑GCGGGGGGGCCATTTTGCAGGGc                                                                          >  4:64006/1‑136 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑ATGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCTTTTTGACGGGc                                                                          >  4:264213/1‑136 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCCGCTGCTTGCGCAAATA‑CTTCACGCCGCGCTCGAAGGCCCCGCCCCCCAGG‑CCGGGCGGGGCGTTTTTGAGGGc                                                                          >  3:95841/1‑136 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCATCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCGTTTTGCACGGc                                                                          >  6:60394/1‑136 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACAACTTAGGCTACGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGG‑CCGGGGTGGCCGTTTTTCAgggg                                                                          >  5:170581/1‑135 (MQ=255)                                                  cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCGCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGGGGGCCGGTTTGCCGGGc                                                                          >  6:67382/1‑136 (MQ=255)                                                               cAACTGTTCCTAACGATCCGT‑CCGCTGCTCTTAAACCCCCTTCGGCTCGGGCGGCTTGCGAAAATC‑CTGCCCGCCGCGCTCGCCGGCCAGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAgg                                                             <  4:177179/136‑1 (MQ=255)                                                               cAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGGAAATA‑CTGCACCCCGCGCTAGAAGGCCAGGCGCGCCTTG‑CCGGGCTGGCGTTTTTGCAGGGCGTGGTTGGCGAgg                                                             >  6:304237/1‑136 (MQ=255)                                                               cAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCCCGCCTGG‑CCGGGCGGGGCTTTTTTGCGGGACGGTTGGccctgg                                                             >  3:7851/1‑132 (MQ=255)                                                                     tttCTCAAGATACGT‑CAGCTGCTCTTCAAACACCTGCGGCTCCGGCTGCTGGCGAAAATA‑CTCCCCGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGttgttg                                                       <  7:311878/136‑1 (MQ=255)                                                                          cAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACGCGCGCCGGG‑CCGGGCTGGGCGGTTTGCAGGGCCGGGTGGCCGGGGTTGTTgaagaa                                                  >  3:217006/1‑136 (MQ=255)                                                                          acaactACGTCCAGTTGCTCTTAATAAACGTTCGGGGCGGGCTGTTGGCCAAATTA‑CTGCCCGCCGCGGGGGCGGCCCGGGGGCGGCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                   <  4:248914/131‑1 (MQ=255)                                                                              ctACGT‑CAGCGGCTCGTCATACCCCTTCGGCTCCGGCTGCTTGCGCAAATC‑CTCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAActct                                              <  8:57247/135‑1 (MQ=255)                                                                                   t‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCCGGCAGGCCGTTTTTCAGGGCCGGTTGGCCGAGGTTGTTTAATAActcaaaat                                          >  2:229769/1‑130 (MQ=255)                                                                                   t‑CAGCTGCTCTTCAAACACCTTCGGCTCCGGCTGCTTGCGAAAATA‑CTCCACGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                         <  1:279848/136‑1 (MQ=255)                                                                                   t‑CAGATGCTATACATACACGTTCGGCTCCGGCGGCTTGCGCAAAAA‑ATGAACGCCGCGCTCGACGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                         <  6:291718/136‑1 (MQ=255)                                                                                     cAGCTGCTTATCATACACCTTCGGCTCGGGCCGCTTGCTCTATTA‑CTGCTGGCCGCGGGCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                        <  7:128050/136‑1 (MQ=255)                                                                                     cAGCGGCGCTTAAAACACCTGGGCCGCGGGCGGCTTGCTAAAATC‑CTGCCCCCGGCGCGCGCCGGCCGGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                        <  6:84401/136‑1 (MQ=255)                                                                                              ttCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGGGGGCCTTTTTTCAGGGCCTGGTGGGCGCGGGTGTTGAAGAACTCGATATGCAGCAGCAcc                               >  1:287948/1‑136 (MQ=255)                                                                                              ttCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCCCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATTCAGCAGCAc                                >  1:266410/1‑135 (MQ=255)                                                                                                        ttCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGCTGGCCTTTTTGCAGGGCCGGGTGGGCGAGGTTGTTGAAGAAAGATATATGCAGCCGCACCCCGATATGGc                     >  8:225063/1‑136 (MQ=255)                                                                                                        ttCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGGTGTTGTATAAATCTATATGCAGCAGCACCAGGATATTGc                     >  2:22925/1‑136 (MQ=255)                                                                                                        tggggCTCGGGGTGCTTGCCAAAATA‑CTCCCCGCCGCGGTCGACGGCCCGGCGCGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                     <  4:84954/133‑1 (MQ=255)                                                                                                          gggCTCGGGCTGCTTGCCAAAATC‑CTCCACGCCGCGCTCGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCg                    <  1:323828/134‑1 (MQ=255)                                                                                                          cgccTCCGGCTGATTGCGCAAATA‑CTGCACCCCGCGCGCGCAGGCCAGTCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                     <  6:301670/131‑1 (MQ=255)                                                                                                          cGGCTCGGGGTGCTGGCCAAAATA‑CGCCACGCCGCGCTCGACGGCCGGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                     <  8:13377/134‑1 (MQ=255)                                                                                                             cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGGCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTTAAGAACTCTATATTGAGCCTCACCAGGCTATGGCGGAtt                >  4:208711/1‑136 (MQ=255)                                                                                                             cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGGTGGCCGAGGGTGTTGTAGAACGCTATATGCAGCAGCACCAGGATATGGCGGAtt                >  5:120065/1‑136 (MQ=255)                                                                                                             cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCGGGGCGTTTTGCAGGGGCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                >  3:62615/1‑136 (MQ=255)                                                                                                             cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                >  6:380181/1‑136 (MQ=255)                                                                                                             cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                >  2:61980/1‑136 (MQ=255)                                                                                                             cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGGTGTTTAAGCACTATATATGCACGAGCCCCCGGATATGGCGGAtt                >  2:160399/1‑136 (MQ=255)                                                                                                              ggggggtgcttgctCAAGTA‑CTGCCCGGCGCGGTCGTTGGGCCGGGGGGGGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt               <  5:347411/130‑1 (MQ=255)                                                                                                              gccGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCCAAGGCCAGGCGCGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt               <  7:84016/135‑1 (MQ=255)                                                                                                               ccGGGTGATTCCCCAAATC‑CTGCCCGCCGGGCTCTACGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTc              <  5:325561/136‑1 (MQ=255)                                                                                                                ggggtgctTGCGAAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt               <  2:266410/130‑1 (MQ=255)                                                                                                                cggcggGTTGCAAAAATC‑CTCCCCGCGGCGCTCGCCGGCCGGGGGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt               <  3:264213/134‑1 (MQ=255)                                                                                                                cGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt               <  6:164488/134‑1 (MQ=255)                                                                                                                cGGCTGCTTGC‑TAAATACCGGAACGCCGCGCTCGAACGCCAGGCGCGCCGGG‑CCGTGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGGTGTTCAAGAACTCTATATGAAGCAGCACCAGGATATGGCGGCttt               <  7:238039/134‑1 (MQ=255)                                                                                                                   ctggtTGCGCAAATC‑CTGCACGCCGCGGTCGAGGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg          <  7:33945/136‑1 (MQ=255)                                                                                                                   ctgctTGCCAAAATC‑CTCCCCGCCGCGCGCGACGCCCGGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg          <  3:49971/136‑1 (MQ=255)                                                                                                                    tgctTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCGGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGa         >  8:248331/1‑136 (MQ=255)                                                                                                                     gctTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc        >  2:125454/1‑136 (MQ=255)                                                                                                                           gcAAATA‑CTGCCCGCCGCGCTCGACGGCCAGGGGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATg  <  8:101048/136‑1 (MQ=255)                                                                                                                                       |                                                                                                                              AATTCGTTGTGCTCGTCTTCTGCCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATG  >  ACB143/2094658‑2094914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑