Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	2,094,808	A→C	49.1%	L186R (CTG→CGG)	CJ019_01995 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,094,808	0	A	C	49.1%	9.3 / 31.8	89	L186R (CTG→CGG)	CJ019_01995	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (26/14);  new base C (19/20);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094680‑2094936                                                                                                                                   |                                                                                                                                  ccGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGCAGGCCCGgcgcgc                                                                                                                            >  1:272004/1‑136 (MQ=255)   ggggCCATGCGGTCCAGTACGTGGCATTTGGCCCGGGCAAACTTTTACTAAATATACGT‑CAGATGCAATTAATACACCTTCGGCTCGGGGTGCTTGCCAAAATA‑CTCCACGCCGCGCTCGACGGCCAGGCGCGCCt                                                                                                                          <  4:329673/136‑1 (MQ=255)       ccATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCCCGCCGGG‑cc                                                                                                                     >  6:194459/1‑136 (MQ=255)        cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTTCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑ccc                                                                                                                    >  3:307623/1‑135 (MQ=255)        cATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCg                                                                                                                    >  8:105071/1‑136 (MQ=255)         agggggTCCAGAACCTGGGCATTGGCCGGGGCCAACAGTTTCTCAAGATACGT‑CCGCTGCTCTTCAAACCCCTTCGGCGCCGGCTGCTTGCGCAAATA‑CTCCCCGCCGCGCTCGACGGCCCGGCGCGCCTGG‑CCgg                                                                                                                   <  6:69377/132‑1 (MQ=255)           ccGGTCCAGTCCCTGGGCATTGCCCCGGGCCAACAGTTGCTCAACATCCTC‑CTGCTGCCATTCATACACCTTCGGCGCCGGCTGCTTGCGAAAATA‑CTCCCCGCCGCGCTCGATGGCCAGGCGCGCCTGG‑CCGGGc                                                                                                                 <  4:141158/135‑1 (MQ=255)              gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTATTCATACACCTTCGGCTCCGGCTCCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACCCGCCCCAGG‑Ccgggcggg                                                                                                              >  3:371795/1‑136 (MQ=255)                  aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGccgt                                                                                                          >  4:266861/1‑134 (MQ=255)                   gTAGCTGGGCATTGGCCGGGCCCATCTTTTTCTAATGTTAGGT‑GCGCGCTTTTTAATACACCTTGGGCGGCGGGGGTTGGCTAAATTA‑CTCCCCCCGGCGCTCGAAGGCCCGCCGCGCCTGG‑CCGGGCTGGCCttt                                                                                                         <  4:113837/136‑1 (MQ=255)                    tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCCGGCTGGCCtttt                                                                                                        >  5:321475/1‑136 (MQ=255)                    tACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCCGGCAGGGCtgtt                                                                                                        >  2:351170/1‑136 (MQ=255)                    tACCTGGGCATTGCCCCGGCCCAACTGTTTCTAAACATCCGT‑CCGCTGCGCTTCATCCACCTTCGGCTCCGGCTGCTGGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCtttt                                                                                                        <  6:298531/136‑1 (MQ=255)                      cgtGGGCATGGGCCGGGCCAAACTGTTTCTAAAGATACGT‑CAGCTGCTCTTCAAACACCTGGGGCGCGGGCTGCTTGCGAAAATC‑CGGCCCGCCGCGCGCGACGGCCCGGCGCGCGGGG‑CCGGGCTGGCCTTTTTg                                                                                                      <  4:124202/134‑1 (MQ=255)                       cggggCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCGGCTCTTCATACACCTGCGGCTCCGGCTGCTTGCGCAAATC‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGc                                                                                                     <  8:295087/134‑1 (MQ=255)                           gCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGGCCCGGGGGGGCGTTTTTgaggg                                                                                                  >  5:316203/1‑132 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCAGGCGCCCCGGG‑GCGGGCGGGCCAGTTTGGAGGGc                                                                                                >  7:78422/1‑136 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCGGGGCGTTTTTGAgggg                                                                                                >  3:164545/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCGGGGCGTTTTGCAgggg                                                                                                >  6:147618/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGGGGGGCGTTTTTAAGGGc                                                                                                >  5:141613/1‑136 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGG‑CCGGGCGGGGCGTTTTGCAgggg                                                                                                >  4:307786/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAgggg                                                                                                >  2:326633/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCGGGGCGTTTTTCAgggg                                                                                                >  2:26525/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑GCCGGCGGGGCGGTTTGGAgggg                                                                                                >  1:313742/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCGTTTTGGAgggg                                                                                                >  5:90944/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACGCGCCCCAGG‑GCGGGGGGGCCATTTTGCAGGGc                                                                                                >  4:64006/1‑136 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑ATGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCTTTTTGACGGGc                                                                                                >  4:264213/1‑136 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCCGCTGCTTGCGCAAATA‑CTTCACGCCGCGCTCGAAGGCCCCGCCCCCCAGG‑CCGGGCGGGGCGTTTTTGAGGGc                                                                                                >  3:95841/1‑136 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCATCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGG‑CCGGGCGGGCCGTTTTGCACGGc                                                                                                >  6:60394/1‑136 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACAACTTAGGCTACGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTGG‑CCGGGGTGGCCGTTTTTCAgggg                                                                                                >  5:170581/1‑135 (MQ=255)                            cATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCGCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGGGGGCCGGTTTGCCGGGc                                                                                                >  6:67382/1‑136 (MQ=255)                                         cAACTGTTCCTAACGATCCGT‑CCGCTGCTCTTAAACCCCCTTCGGCTCGGGCGGCTTGCGAAAATC‑CTGCCCGCCGCGCTCGCCGGCCAGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAgg                                                                                   <  4:177179/136‑1 (MQ=255)                                         cAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGGAAATA‑CTGCACCCCGCGCTAGAAGGCCAGGCGCGCCTTG‑CCGGGCTGGCGTTTTTGCAGGGCGTGGTTGGCGAgg                                                                                   >  6:304237/1‑136 (MQ=255)                                         cAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCCCGCCTGG‑CCGGGCGGGGCTTTTTTGCGGGACGGTTGGccctgg                                                                                   >  3:7851/1‑132 (MQ=255)                                               tttCTCAAGATACGT‑CAGCTGCTCTTCAAACACCTGCGGCTCCGGCTGCTGGCGAAAATA‑CTCCCCGCCGCGCTCGAAGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGttgttg                                                                             <  7:311878/136‑1 (MQ=255)                                                    cAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCACGCGCGCCGGG‑CCGGGCTGGGCGGTTTGCAGGGCCGGGTGGCCGGGGTTGTTgaagaa                                                                        >  3:217006/1‑136 (MQ=255)                                                    acaactACGTCCAGTTGCTCTTAATAAACGTTCGGGGCGGGCTGTTGGCCAAATTA‑CTGCCCGCCGCGGGGGCGGCCCGGGGGCGGCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                                         <  4:248914/131‑1 (MQ=255)                                                        ctACGT‑CAGCGGCTCGTCATACCCCTTCGGCTCCGGCTGCTTGCGCAAATC‑CTCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAActct                                                                    <  8:57247/135‑1 (MQ=255)                                                             t‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCCGGCAGGCCGTTTTTCAGGGCCGGTTGGCCGAGGTTGTTTAATAActcaaaat                                                                >  2:229769/1‑130 (MQ=255)                                                             t‑CAGCTGCTCTTCAAACACCTTCGGCTCCGGCTGCTTGCGAAAATA‑CTCCACGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                               <  1:279848/136‑1 (MQ=255)                                                             t‑CAGATGCTATACATACACGTTCGGCTCCGGCGGCTTGCGCAAAAA‑ATGAACGCCGCGCTCGACGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                               <  6:291718/136‑1 (MQ=255)                                                               cAGCTGCTTATCATACACCTTCGGCTCGGGCCGCTTGCTCTATTA‑CTGCTGGCCGCGGGCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                              <  7:128050/136‑1 (MQ=255)                                                               cAGCGGCGCTTAAAACACCTGGGCCGCGGGCGGCTTGCTAAAATC‑CTGCCCCCGGCGCGCGCCGGCCGGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgc                                                              <  6:84401/136‑1 (MQ=255)                                                                        ttCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGGGGGCCTTTTTTCAGGGCCTGGTGGGCGCGGGTGTTGAAGAACTCGATATGCAGCAGCAcc                                                     >  1:287948/1‑136 (MQ=255)                                                                        ttCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCCCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATTCAGCAGCAc                                                      >  1:266410/1‑135 (MQ=255)                                                                                  ttCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCAGG‑CCGGGCTGGCCTTTTTGCAGGGCCGGGTGGGCGAGGTTGTTGAAGAAAGATATATGCAGCCGCACCCCGATATGGc                                           >  8:225063/1‑136 (MQ=255)                                                                                  ttCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCCGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGGTGTTGTATAAATCTATATGCAGCAGCACCAGGATATTGc                                           >  2:22925/1‑136 (MQ=255)                                                                                  tggggCTCGGGGTGCTTGCCAAAATA‑CTCCCCGCCGCGGTCGACGGCCCGGCGCGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                                           <  4:84954/133‑1 (MQ=255)                                                                                    gggCTCGGGCTGCTTGCCAAAATC‑CTCCACGCCGCGCTCGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCg                                          <  1:323828/134‑1 (MQ=255)                                                                                    cgccTCCGGCTGATTGCGCAAATA‑CTGCACCCCGCGCGCGCAGGCCAGTCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                                           <  6:301670/131‑1 (MQ=255)                                                                                    cGGCTCGGGGTGCTGGCCAAAATA‑CGCCACGCCGCGCTCGACGGCCGGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                                           <  8:13377/134‑1 (MQ=255)                                                                                       cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGGCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTTAAGAACTCTATATTGAGCCTCACCAGGCTATGGCGGAtt                                      >  4:208711/1‑136 (MQ=255)                                                                                       cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGGTGGCCGAGGGTGTTGTAGAACGCTATATGCAGCAGCACCAGGATATGGCGGAtt                                      >  5:120065/1‑136 (MQ=255)                                                                                       cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGG‑CCGGGCGGGGCGTTTTGCAGGGGCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                                      >  3:62615/1‑136 (MQ=255)                                                                                       cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                                      >  2:61980/1‑136 (MQ=255)                                                                                       cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAtt                                      >  6:380181/1‑136 (MQ=255)                                                                                       cTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGGTGTTTAAGCACTATATATGCACGAGCCCCCGGATATGGCGGAtt                                      >  2:160399/1‑136 (MQ=255)                                                                                        ggggggtgcttgctCAAGTA‑CTGCCCGGCGCGGTCGTTGGGCCGGGGGGGGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  5:347411/130‑1 (MQ=255)                                                                                        gccGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCCAAGGCCAGGCGCGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  7:84016/135‑1 (MQ=255)                                                                                         ccGGGTGATTCCCCAAATC‑CTGCCCGCCGGGCTCTACGGCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTc                                    <  5:325561/136‑1 (MQ=255)                                                                                          ggggtgctTGCGAAAATA‑CTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  2:266410/130‑1 (MQ=255)                                                                                          cggcggGTTGCAAAAATC‑CTCCCCGCGGCGCTCGCCGGCCGGGGGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  3:264213/134‑1 (MQ=255)                                                                                          cGGCTGCTTGCGCAAATA‑CTGCCCGCCGCGCTCGAAGGCCTGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  6:164488/134‑1 (MQ=255)                                                                                          cGGCTGCTTGC‑TAAATACCGGAACGCCGCGCTCGAACGCCAGGCGCGCCGGG‑CCGTGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGGTGTTCAAGAACTCTATATGAAGCAGCACCAGGATATGGCGGCttt                                     <  7:238039/134‑1 (MQ=255)                                                                                             ctggtTGCGCAAATC‑CTGCACGCCGCGGTCGAGGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg                                <  7:33945/136‑1 (MQ=255)                                                                                             ctgctTGCCAAAATC‑CTCCCCGCCGCGCGCGACGCCCGGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTg                                <  3:49971/136‑1 (MQ=255)                                                                                              tgctTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCGGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGa                               >  8:248331/1‑136 (MQ=255)                                                                                               gctTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc                              >  2:125454/1‑136 (MQ=255)                                                                                                     gcAAATA‑CTGCCCGCCGCGCTCGACGGCCAGGGGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATg                        <  8:101048/136‑1 (MQ=255)                                                                                                                   gccgccCTCGAAGCCCAGGCGCCCGGGG‑CAGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCCGATTTCCTTGACCCTATGCTTGGCCTCGACa           <  5:320229/136‑1 (MQ=255)                                                                                                                   gccgcGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGGCTGTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGAcc           >  5:259459/1‑135 (MQ=255)                                                                                                                   gccgcGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa           >  7:227574/1‑136 (MQ=255)                                                                                                                   gccgcGCTCGAAGGCCACGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCACCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa             >  7:253376/1‑134 (MQ=255)                                                                                                                   gccgcGCGCGAAGCCCCGGCGCCCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa           <  5:190654/136‑1 (MQ=255)                                                                                                                   cccgcGCTCGACGCCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa           <  5:184475/135‑1 (MQ=255)                                                                                                                         cgcgctggCCAGGCGGGCGTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc     <  2:256447/130‑1 (MQ=255)                                                                                                                         cgccacggCCCGCCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc     <  6:141613/130‑1 (MQ=255)                                                                                                                         cccGACGGCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc     <  5:12751/134‑1 (MQ=255)                                                                                                                         cTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGGCTTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGATGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTACTTTACCCAATGCTTGGCCTCGTCAGCggg      >  6:104833/1‑135 (MQ=255)                                                                                                                          tCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTACTTTACCCAAAGCTTGGCCTCGACAGCGGGc     >  4:348505/1‑135 (MQ=255)                                                                                                                           cgccggCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaa   <  4:164545/132‑1 (MQ=255)                                                                                                                            gcgggCCTGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGACTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  4:155954/133‑1 (MQ=255)                                                                                                                            gccggCCCGCCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  1:22925/133‑1 (MQ=255)                                                                                                                            gatggCCAGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  8:265845/133‑1 (MQ=255)                                                                                                                            gacggCCCGGCGCGCGGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGTGGATTTCCTTGACCCAATGCTTGGCCTCGGCAGCGGGCaag  <  6:377483/133‑1 (MQ=255)                                                                                                                            gacggCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  6:312378/133‑1 (MQ=255)                                                                                                                            gAAGGCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  4:309354/136‑1 (MQ=255)                                                                                                                              tggCCCGGCGCGCCGGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  2:342547/133‑1 (MQ=255)                                                                                                                              cggCCCGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  5:69879/133‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  CCGGGGCCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGT‑CAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATA‑CTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGG‑CCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094680‑2094936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑