BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	4,844,971	T→G	26.1%	intergenic (+47/+72)	apt → / ← recR	Adenine phosphoribosyltransferase/Recombination protein RecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,844,971	0	T	G	26.1%	140.8 / 17.7	151	intergenic (+47/+72)	apt/recR	Adenine phosphoribosyltransferase/Recombination protein RecR
Reads supporting (aligned to +/- strand): ref base T (22/77); new base G (28/10); total (59/92)
Fisher's exact test for biased strand distribution p-value = 6.34e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

CCGGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCAT  >  ACB143/4844841‑4845102

ccGGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCt                                                                                                                                        <  4:315143/134‑1 (MQ=255)

ccGGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTAc                                                                                                                                      <  3:358089/136‑1 (MQ=255)

 cGGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACg                                                                                                                                     <  5:70768/136‑1 (MQ=255)

 cGGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACg                                                                                                                                     <  2:121890/136‑1 (MQ=255)

  ggAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑CCGGCTACg                                                                                                                                     >  2:216047/1‑135 (MQ=255)

  ggAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑AGCCGGT‑TAAACCC‑CCGCCTAc                                                                                                                                      >  2:159218/1‑135 (MQ=255)

   gAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGaa                                                                                                                                   <  7:374152/136‑1 (MQ=255)

         gACGGGTCGCGTCGGTTGGAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑Cc‑gc                                                                                                                           <  2:357320/136‑1 (MQ=255)

         gACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑Cc‑gc                                                                                                                           <  8:367774/136‑1 (MQ=255)

           cGGGTCGCGTGGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑Gt                                                                                                                        <  6:337382/136‑1 (MQ=255)

           cGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑Gt                                                                                                                        <  6:139926/136‑1 (MQ=255)

             gggcgggTCGGTGGCACGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTAt                                                                                                                      <  1:387440/130‑1 (MQ=255)

             ggTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAAACC‑GCGCCGACGGAAG‑CC‑GC‑GTAt                                                                                                                      >  7:253223/1‑136 (MQ=255)

                 gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTTGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGGTTAAACCC‑CTACATAGAAAAG‑CC‑GG‑GTGTG‑tt                                                                                                                  >  3:214100/1‑136 (MQ=255)

                 gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCAGCGAAGAAAG‑CC‑GC‑CTATG‑ttt                                                                                                                 >  6:156764/1‑136 (MQ=39)

                 gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAAACC‑GCACCTACGAAAG‑CC‑GC‑GGATG‑tgt                                                                                                                 >  2:119748/1‑134 (MQ=255)

                    tCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑GCGCCAACGCAAG‑CC‑GC‑GGATG‑tttttt                                                                                                              >  4:304887/1‑134 (MQ=39)

                     cgttggCAAGCGGCCGGGTTGCCTACCTTCTGCCTGACCGAGTTATCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTa                                                                                                             <  8:303748/131‑1 (MQ=255)

                     cGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTa                                                                                                             <  8:188273/136‑1 (MQ=255)

                      ggTTCCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTa                                                                                                             <  7:93118/135‑1 (MQ=255)

                       gTTGCAAGCGGCCGGGGGGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAgg                                                                                                           <  6:169223/136‑1 (MQ=255)

                           aaaGCGGACGGGGTGCCTACATTCTGCCTTACCGAGTTTTCCCTCAGCGCGTATTGATTGGGCATTTGGGGC‑TGA‑ATTTCAGCACAAT‑‑CG‑CCGG‑TAAAACC‑CCGCCGAAGAAAA‑CC‑GC‑GGATT‑TTTTTTATgcg‑cc                                                                                                      >  2:263309/2‑135 (MQ=255)

                                ggCCGGGGTGCCTACCTTCTGCCTGCCCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAACCCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGttt                                                                                                 <  3:54352/136‑1 (MQ=255)

                                ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCTGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGGTTAAACCC‑CCGCACAGGAAAC‑CC‑CC‑GTTTT‑TTTTTTAGGGG‑GGGGtt                                                                                                  >  6:135253/1‑136 (MQ=255)

                                ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATTAGCG‑GGGG‑TAAACCC‑‑CTCGAACAAAAG‑CC‑GC‑GAGTT‑TTTTTTTGGCg‑gggtgt                                                                                                  >  4:15870/1‑133 (MQ=255)

                                ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAAACC‑CCGCCGACCCAAG‑CC‑GC‑GGATG‑TTTTTTTGGGG‑CGGGgtt                                                                                                 >  4:141549/1‑136 (MQ=255)

                                ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑AG‑CGGG‑GTAACCC‑GCGCCCAACGAAG‑CC‑GC‑GGTTG‑TGTTTTTGGGG‑CGGGgtt                                                                                                 >  7:21234/1‑136 (MQ=255)

                                    ggggTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑GCGCCGACGGAAG‑CC‑GC‑GGTTG‑TGTTGTAGGCG‑CGGGGTTAccc                                                                                             >  5:266157/1‑136 (MQ=38)

                                                      ctcaCCGAGTTTCCCCTCAGCGAGTATTGCGTGGGCTTTTGGGGC‑TGC‑TTTCCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTCCGAACG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGc                                                                           <  4:84109/133‑1 (MQ=38)

                                                       tGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑CCTCCTACACAAA‑CC‑GC‑CGATG‑TTTTTTATGCG‑CGGGTTTTACCCCCAAAGGgccgcccaccg                                                                          >  6:96373/1‑131 (MQ=25)

                                                        gACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAAACC‑GCTGCTACGAAAG‑CC‑GC‑GGATG‑TTTTGTTTGCG‑CGGGGTTTCCCGCGCAAGGGCCGCCaacagg                                                                         >  2:109702/1‑132 (MQ=37)

                                                        gACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAAACC‑CCGCCAACCAAAT‑CC‑GC‑CGATG‑TTTTTTAAGCG‑CGGGTTTAACCGCCAAATGGCCGCCAACCgg                                                                         >  3:44475/1‑136 (MQ=255)

                                                          ccGAGTTTTCCCTCTCCGAGTATTGAGTGGGCTTGTGGGGC‑GGC‑TTGGCAGCCAATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAC‑CC‑GC‑GTATG‑TTTTGTAGGCC‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGcc                                                                       <  2:279331/136‑1 (MQ=37)

                                                           cGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GGATG‑TTTTGTAGGCG‑CCGGGTTACCCGCGAATGGGCCGCCCAGCGGccc                                                                      >  1:252567/1‑136 (MQ=255)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑GCTCCGACGCAAC‑CC‑GC‑GGATG‑GTTTTTAGGCG‑CGGGGTTTCCCGCGAATGGGGCCCCCAGCGGcccc                                                                     >  5:392701/1‑136 (MQ=37)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑GCGCCAAACCAAG‑CC‑CC‑CGATG‑TTTTTTTTGCG‑CGGGTTTTCCCCCCAAATGGCCCCCCACCGGgccc                                                                     >  5:302619/1‑136 (MQ=16)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑CCTCCGACGAAAG‑CC‑GC‑GTTTG‑TTTTTTAAGCG‑CGGGGTTAACCGCGAAAGGGCCGCCCAGCGGccc                                                                      >  1:48927/1‑135 (MQ=37)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑CCGCCAACGAAAA‑CC‑GC‑GGATT‑TTTTTTATGCG‑CGGGGTTTACCGCGAATTGGCCGCCCAGCGGgcc                                                                      >  5:363973/1‑135 (MQ=255)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑CCCCCTAACAAAG‑CC‑CC‑GTATT‑TTTTTTTGGCG‑CGGGTTTACCCCCGAATGGGCCGCCAAACCGcccc                                                                     >  6:267263/1‑136 (MQ=37)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAAACC‑CCTCCGAACCAAG‑CC‑CC‑GGTTGTTTTTTTTGCCC‑CGGGTTTACCC‑CGAAAGGGGCCCCCACCGGcccc                                                                     >  3:165137/1‑136 (MQ=255)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAAACC‑GCTCCAACCAAAC‑CC‑GC‑GGATG‑TTTTTTAGGCG‑CGGGTTTACCCCCGAATGGGCCGCCCACCCGcccc                                                                     >  8:188238/1‑136 (MQ=37)

                                                            gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCACATT‑‑CG‑CGGG‑TTAACCC‑GCTCCGACGAAAG‑CC‑GC‑GGATG‑TTTTGTAGGCG‑CGGGTTTTACCGCGCATGGGGCGCCCAGCCGcccc                                                                     >  5:91497/1‑136 (MQ=255)

                                                                 ttCCCTCAGCGAGTATGGAGTGGGCTTTTGGGGC‑GGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑AAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTGGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATg                                                                 <  5:103297/135‑1 (MQ=38)

                                                                          gtaGTTTTGAGTGGGCTTTGGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAACCCC‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑g                                                      <  6:264861/134‑1 (MQ=38)

                                                                          cGATTATTGCGGGGGCTTTTGGGGG‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAACCCC‑GAGCCTCCGAACG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑g                                                      <  8:253223/136‑1 (MQ=37)

                                                                           gAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAAACC‑GCGGCTAAGAAAG‑CC‑GC‑GTATT‑TTTTTTATGCC‑CGGGGTTTCCCGCGGATTGGCCCCCAAGCGGCCCCCATGAATCAGAGC‑g                                                      >  8:80066/1‑135 (MQ=255)

                                                                           gAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAAACC‑CCTGCGTCGCAAG‑CC‑GC‑GGTTG‑TTTTGTAGGCG‑CGGGGTTTCCCGCGAATGGGCCCCCCACCGGCCCCAATGAATAAGcgc‑g                                                      >  7:35531/1‑135 (MQ=255)

                                                                           gAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GGATT‑TTTTGTTGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATAAGAGC‑g                                                      >  5:183453/1‑135 (MQ=255)

                                                                           gAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTAACTAAG‑GC‑GC‑GTATG‑TTTTGTAGGAG‑CCGGTTTAACCGCCAATTGGGCGCCAAACCGCCCCAAAGAATCAGAGC‑g                                                      >  7:378865/1‑135 (MQ=25)

                                                                               aTTGAGTGCGGTTTTGGGGC‑GTC‑TTCCCA‑CCAATTG‑CC‑CGGT‑AAAACCC‑GCTCAAACTAAAG‑CG‑GC‑TTTTG‑TTTTGTCGCCG‑GGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATg                                                 <  4:395567/136‑1 (MQ=255)

                                                                                    gTGGGCTTTTGGGGC‑GGC‑TGTGCCGCCCATT‑‑CG‑CGGG‑TAACCCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg                                            <  7:167221/136‑1 (MQ=38)

                                                                                     tGGGGTTTTGGGGC‑GGC‑TTTGCTGCCCATT‑‑GG‑GGGG‑TAAACCC‑GCGCCTACGAACG‑CG‑GC‑GTATG‑TTTTGATGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg                                            <  5:181469/135‑1 (MQ=37)

                                                                                     tGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CGCCGGG‑TAAACCC‑‑CGCCATCGCAAGCCC‑GG‑GTTTG‑TTTT‑TTGGCGCCGGGTTTGCCCCCCAAGGGGCC‑CCCACCGGGCCCAATTAATACGCGC‑GCAATTGGCCgg                                           >  8:156446/1‑136 (MQ=255)

                                                                                     tGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg                                            <  8:106378/135‑1 (MQ=255)

                                                                                     gtgggttttGGGGG‑GGCATTTGCCCCCAATC‑‑CG‑C‑GG‑TAAACCC‑CCCCCAACCCAAC‑CCGGC‑GTTTT‑TTTTGAGGGCG‑GGGTTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg                                            <  5:32174/131‑1 (MQ=255)

                                                                                     gggggTTTGGGGGC‑TGG‑TTTCCCGCCCTTG‑‑CG‑CGGG‑TAACCCC‑GCTCCTACCAACG‑CC‑GC‑TTATT‑TTTTGTAGGGG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg                                            <  7:233511/134‑1 (MQ=25)

                                                                                     ggggCTTTGGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCg                                            <  2:263323/134‑1 (MQ=255)

                                                                                      tgggttttGGGGCGTTC‑TTTGCTGCCCTTC‑‑CG‑CGGG‑TAACCCC‑GCTCTTCCGAACG‑CC‑GC‑TTTTG‑TTTTGTAGGGG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCgg                                           <  8:227611/132‑1 (MQ=25)

                                                                                       ggCTTTTGGGGC‑TGC‑TTTCCAGCCAATT‑‑CG‑CGGG‑AAACCCC‑GCTCCTACCAACC‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGgc                                          <  4:340065/135‑1 (MQ=38)

                                                                                           tttGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTGCTACGAAAG‑CC‑GC‑GGATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCt                                     >  3:293872/1‑136 (MQ=255)

                                                                                            ttGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGTAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGGATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCt                                     <  7:7712/135‑1 (MQ=255)

                                                                                                ggC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑GCTGCTACGAAAG‑CC‑GC‑GGTTG‑TTTTGTAGGCG‑CGGGGTTACCCGCGCATGGGCCGCCCAGCGGCCCCAATGGATCAGAGC‑GATATGGGCCGGCGCCCGGCa                                  >  3:304530/1‑134 (MQ=37)

                                                                                                ggC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TTAAACC‑CCGCCGACGGAAG‑GC‑GCGGTTTG‑TTTTTTTGGGC‑GGGGTTTACCGCGGATTGGGCCCCCACCCGG‑CCCACAGAATGAGCGCGGAAATGGGCC‑GCGccccggaa                                 >  4:270630/1‑130 (MQ=255)

                                                                                                 gc‑tgc‑tTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg                               <  1:309734/136‑1 (MQ=255)

                                                                                                 gc‑tgc‑tTTGCAGCCCATT‑‑CG‑CGGG‑AAAACCC‑GCGCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg                               <  7:279688/136‑1 (MQ=255)

                                                                                                 gc‑tgc‑tTTCCAGCCCTTT‑‑CG‑CGGG‑TAACCCC‑GCTCCTCCGAAAG‑CC‑GC‑TTTTG‑TTTTGTCGGGG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg                               <  8:36434/136‑1 (MQ=38)

                                                                                                 gc‑tgc‑tTGGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg                               <  2:61475/136‑1 (MQ=255)

                                                                                                  ggggc‑tTTGCAGCCCATT‑‑CG‑CGGG‑AAAACCC‑GCTCATACGAAAG‑CC‑GC‑GTTTG‑TTTTGAAGGCG‑CGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg                               <  4:353100/133‑1 (MQ=255)

                                                                                                  gcggc‑tTTGCAGCCCATT‑‑CG‑CGGC‑TAACCCC‑GATCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg                               <  1:101953/133‑1 (MQ=255)

                                                                                                  c‑tgc‑tTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACTAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAgg                              <  1:55620/136‑1 (MQ=255)

                                                                                                   gcggatttGCAGCCCATT‑‑CG‑CGGT‑AAACCCC‑GCTCCTCCGAACG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑GGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAg                               <  7:154047/131‑1 (MQ=255)

                                                                                                      c‑tTTGCAGCCCATT‑‑CG‑CGGG‑TTAACCC‑GCGGCGACGGAAG‑CC‑GG‑GGTTG‑TTTTGTTGGCG‑CGGGGTTTCCCGCGAATGGGGCGGCCCGCGGGCCCCATGGATGAGGGG‑GGAATGGGGCCGCGCCCGGGAAAGGGAt                           >  8:263892/1‑136 (MQ=255)

                                                                                                        tttGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCGGCTACGAAAG‑GC‑GC‑GGTTG‑GTTTGTTGGCG‑CGGGGTTTCCCGCGAATGGGGCGCCCAGCGGGCCCCATGGATCAGAGC‑GCAATGGGCCGGCGCCCGGGCAAGGGATg                          >  6:374516/1‑136 (MQ=255)

                                                                                                        tttGCAGCCCATT‑‑CG‑CGGG‑AAACCCC‑GCTCCAACGAACG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑CGGGTTTCCCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  8:121688/136‑1 (MQ=255)

                                                                                                        tttGCAGCCCATT‑‑CG‑CGGG‑AAAACCC‑GCTCCTACGAACG‑CC‑GC‑GTAGG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  7:314865/136‑1 (MQ=255)

                                                                                                        tttCCAGCCCATT‑‑GG‑CGGG‑TAAACCC‑GCTCCTACTAACG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  5:386537/136‑1 (MQ=39)

                                                                                                        ttggCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCGCCTACTAAAG‑CC‑GC‑GTATG‑TTTTTTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCAGCAAAGGCATg                          <  4:332060/133‑1 (MQ=38)

                                                                                                        gtcgctgCCCTTT‑‑GG‑CGGG‑TACACCC‑GCTCCAACGAAAG‑CC‑GC‑GTATT‑TTTTGTCGGCG‑GGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  4:210564/130‑1 (MQ=255)

                                                                                                        gcggCACCCCATG‑‑CG‑CGGT‑ACAACCC‑GCGCATCCCAATG‑CC‑CC‑GTATG‑TTGTGACGGCG‑CGGTTATACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  8:333960/133‑1 (MQ=255)

                                                                                                         ttggcccccATT‑‑GG‑GGGG‑TAAACCCGGCTCCACCGAACG‑CC‑GC‑TTTTG‑TTTTGTAGGGG‑GGGTTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  3:197687/130‑1 (MQ=255)

                                                                                                         ttGCAGCCCATT‑‑CG‑CGGG‑GTAAACC‑GCGGCGTCGGAAG‑GC‑GG‑GGTTG‑TTTTGTTGGCG‑GGGGGTTTCCCGCGGATGGGGCGCCCAGCGGGCCCCATGGATGAGAGC‑GCAATGGGGCGGCGCCCGGGAAAgggaggg                         >  6:193721/1‑131 (MQ=255)

                                                                                                          tGCAGCCCATT‑‑CG‑CGGG‑TAACCCC‑GCTCCTACTAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  8:232609/134‑1 (MQ=255)

                                                                                                          tGCAGCCCATT‑‑CG‑CGGG‑TAACCCC‑GCGCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  1:136977/134‑1 (MQ=255)

                                                                                                          tGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  8:128026/134‑1 (MQ=255)

                                                                                                          tGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGAAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  7:18316/134‑1 (MQ=255)

                                                                                                          tGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCGCCTACGAAAG‑CC‑GC‑GTTTG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  2:8185/134‑1 (MQ=255)

                                                                                                          tGCAGCCCATT‑‑CG‑CGGG‑AAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATTGGCCGGCGCCCTGCAAAGGCATg                          <  2:256620/134‑1 (MQ=255)

                                                                                                          tGCACCCCATT‑‑CG‑CGGG‑CAAACCC‑GCTCCTACCAAAG‑CC‑GC‑TTATG‑TTTTGACGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  5:206888/134‑1 (MQ=39)

                                                                                                          ggCAGCCCATT‑‑CG‑CGGG‑AACACCC‑GCGCCTACGAAAC‑CC‑GC‑GTATG‑TTTTGTCGGCG‑GGGTTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATg                          <  1:384515/133‑1 (MQ=255)

                                                                                                           gCAGCCCATT‑‑CG‑CGGG‑AAAACCC‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATggg                        <  1:49092/135‑1 (MQ=255)

                                                                                                             aGCCCATT‑‑CG‑CGGG‑TAAACCG‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGTCGGCG‑CGGGTTAACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCa                     <  2:313617/136‑1 (MQ=255)

                                                                                                                    t‑‑CG‑GGGC‑TAAACCC‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAc               <  6:178916/135‑1 (MQ=255)

                                                                                                                       tcccgGG‑TACACCG‑GCTCCACCGACCG‑CC‑GG‑GTTTG‑TGTGGTCGGCG‑CGGGTATCCCGGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTa                <  4:102381/131‑1 (MQ=255)

                                                                                                                        g‑cgGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAccgc            <  2:287538/136‑1 (MQ=255)

                                                                                                                           ggg‑TAAACCC‑GCTCCTACTAACG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAccgccg          <  7:267054/136‑1 (MQ=255)

                                                                                                                            gg‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGt         <  2:250873/136‑1 (MQ=255)

                                                                                                                             gttAAACCC‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGAAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGt         <  1:220502/134‑1 (MQ=255)

                                                                                                                               tAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTcc       <  6:357226/136‑1 (MQ=255)

                                                                                                                                aaaCCC‑GCGCCTGCGAAAG‑GC‑GC‑GGATG‑TTTTGTAGGCG‑CGGGGTTTGCCGCGCATGGGGCGCCAAGCGGCCCCAATGAATCAGAGG‑GAAATGGGCCGGCGCCCTGCAAAGGCAGGGGCCCGGGTGCCGCCCTccc      >  2:68144/1‑135 (MQ=255)

                                                                                                                                    cc‑GCTCCTACGAACG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCAt  <  1:345034/136‑1 (MQ=255)

CCGGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGC‑TGC‑TTTGCAGCCCATT‑‑CG‑CGGG‑TAAACCC‑GCTCCTACGAAAG‑CC‑GC‑GTATG‑TTTTGTAGGCG‑CGGGTTTACCCGCGAATGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑GAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCACCAT  >  ACB143/4844841‑4845102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`