| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,537,415 | 0 | C | G | 36.0% | 27.2 / 12.4 | 25 | A388G (GCG→GGG) | mdfA | Multidrug transporter MdfA |
| Reads supporting (aligned to +/- strand): ref base C (14/2); new base G (0/9); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCT > ACB143/1537290‑1537541 | cGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGc > 5:178955/1‑135 (MQ=255)cGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCGTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGACC‑TGGCGGCGGGGGT‑GGc > 5:4538/1‑135 (MQ=255) agagCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCggggg > 4:121739/1‑135 (MQ=255) gCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCt > 5:301517/1‑135 (MQ=255) gTTGCGGCCATGCTGGGGATGATCAGGATGGCCTTGGTGGGGTTGGGCGGAGGGCTGGTGGGGATGATGGGCGCGGGGGCCAGGTTGCTGCATTTTGCCC‑TGGCGGGGGGGGT‑GGCGGGGGCGTTGGCGCTGTgg < 6:325036/135‑1 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGc > 7:346850/1‑135 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGc > 7:328832/1‑135 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGc > 2:248632/1‑135 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGc > 6:82841/1‑135 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGc > 5:30737/1‑135 (MQ=255) ttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGTAGCGCTGCTGGCGATTATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGcc < 3:352432/135‑1 (MQ=255) ttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCGGCTGGCGATGATTGGCGCGGGGGCCAGCTTGCTGCTTTTTGCCC‑TGGCGGGGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGcc < 3:234507/135‑1 (MQ=255) gcgggggTTGATCCGGTTTGCGTTGGTGGGGTTGGGGGGCGGGGGGGGGGCTTTGTTTGGCGCCGGTGCATGCTTGCTGCTTTTTGCCC‑TGGGGGGGGGGGG‑GGGGGGGGCGTTGGCGCTGTGGCCGCTGTGGt < 6:154412/131‑1 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGtgggtggg > 5:217838/1‑135 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGtgggtggg > 3:245679/1‑135 (MQ=255) aCGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGtgggtggg > 2:55766/1‑135 (MQ=255) tGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGCCGCGGGGGTTTGTGGTGGGTGGGCGGCCGgggg > 7:305073/1‑135 (MQ=255) ctgctgGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATaa > 1:140421/1‑135 (MQ=255) tgcTTTTTGCCG‑GGGGGGGGGGGGG‑GGGGGGGGCGTGGGCGCTGGGGCGGGTGTGTTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 7:261202/135‑1 (MQ=255) tgaTTTTTCCC‑GGGGGGGGGGGGTGGGGGGGGGGGGTGGGGCTGTGGCGGGGGTGGTGTGTGGTGGGTGGGGGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 6:100358/133‑1 (MQ=255) tgaTTTTGCCCGGGGGGGGGGGGGG‑GGGGGGGGCGTGGGGGGGGTGGCGGGTGGGGTTGGGGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 4:124617/133‑1 (MQ=255) tttttGCCG‑GGGGGGGGGGGGG‑GGGGGGGGGTTGGGCGCGGTGGCCGAGGGGGTTGGGGGTGGGTGGGCGCCCGGGGTACGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAc < 1:329341/133‑1 (MQ=255) ttttgGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGGGGCCGCTGGGGTTGGGGGTGGGTGGGCGCCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAcc < 4:147491/134‑1 (MQ=255) ttttGCCCGGGGGGGGGGGGGG‑GGGGGGGGGGTGGGCGCGGTGGCCGCGGTGTTTTGGGGGGGGTGGGCGGCGGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAAc < 8:268397/134‑1 (MQ=255) cgtGGGGGGGGGGGG‑GGGGGGGGGGGGGGCGCGGTGGCCGCGGGGGGTTGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCt < 1:248632/133‑1 (MQ=25) | CGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGT‑GGCGGGGGCGTTGGCGCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCT > ACB143/1537290‑1537541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |