Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,844,971	0	T	G	38.0%	74.9 / 16.8	97	intergenic (+47/+72)	apt/recR	Adenine phosphoribosyltransferase/Recombination protein RecR
Reads supporting (aligned to +/- strand):  ref base T (12/45);  new base G (28/7);  total (42/55)
Fisher's exact test for biased strand distribution p-value = 2.89e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCC  >  ACB143/4844843‑4845097                                                                                                                                    |                                                                                                                                       ggAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTccaaa                                                                                                                                   >  2:246451/1‑131 (MQ=255) ggAACGGCACGGGTCGCGGCGTTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACg                                                                                                                                  <  4:135739/135‑1 (MQ=255)  gAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCGCGCCAAcc                                                                                                                                  >  6:101873/1‑133 (MQ=255)   aaCTGGACGGGTCGCGGCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGaa                                                                                                                                <  6:293415/135‑1 (MQ=255)   aaCTGGACGGGGCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGaa                                                                                                                                <  8:321428/135‑1 (MQ=255)     gtGGACGGGGCGCGGGGGTGCCAAGCGGCCGGGGGGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAg                                                                                                                              <  4:7290/134‑1 (MQ=38)      tGGACGGGTCGCGTGGTTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGc                                                                                                                             <  7:225334/135‑1 (MQ=255)        gaggggtcgggtcGTTTGAAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCcg                                                                                                                           <  6:219291/132‑1 (MQ=255)        gACGGGTCGCGGCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCcg                                                                                                                           <  7:255838/135‑1 (MQ=255)         aCGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCcgc                                                                                                                          <  4:281487/135‑1 (MQ=255)           gggTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAACCCCCGGCGACGCAAGCCGC‑Gt                                                                                                                       >  7:99274/1‑135 (MQ=255)           gggTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCGCCTACGAAAGCccc‑gg                                                                                                                       >  8:54017/1‑134 (MQ=255)           gggTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCCCGCCAGAGCAAGCcgc‑gg                                                                                                                       >  5:89335/1‑134 (MQ=255)                gcggcgGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑tt                                                                                                                 <  5:164177/135‑1 (MQ=255)                gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTACC‑G‑GGGTAAACCC‑CGCCAACGCAAGCcgc‑ggttggt                                                                                                                  >  4:355811/1‑129 (MQ=255)                gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCGCTCCGACGAAAGCCGC‑Ggttg‑tt                                                                                                                 >  7:214939/1‑135 (MQ=39)                gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCGCTACTAAGAAAGCCGC‑GGATG‑tt                                                                                                                 >  5:184247/1‑135 (MQ=39)                gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCGCGCCATCGAAAGCCGG‑GGATggt                                                                                                                  >  4:123074/1‑133 (MQ=255)                gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCGCACCGAAGAAATCCGC‑GGATG‑tt                                                                                                                 >  4:242232/1‑135 (MQ=255)                gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGGAAAACCCCGCCATCGGAAACCGC‑GGATG‑tt                                                                                                                 >  1:340157/1‑135 (MQ=255)                gcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑AG‑C‑GGGGAAAACCGCGCCCTCGCAAGCCGC‑GGATggt                                                                                                                  >  7:191505/1‑133 (MQ=255)                 cggcGGTTGAAAGCGTCGGGGGTGCCTACCTTCTGCCTCACCGAGTTTTCCCACAGCTATTATTGCGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑ttt                                                                                                                <  7:312163/132‑1 (MQ=37)                 cgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑ttt                                                                                                                <  3:143348/135‑1 (MQ=255)                      gtggCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAg                                                                                                          <  3:242481/132‑1 (MQ=255)                      gTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAg                                                                                                          <  5:196742/135‑1 (MQ=255)                       ttGCACGCGGCCGGGGTGCCTACCTTCTCCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAgg                                                                                                         <  4:124576/135‑1 (MQ=255)                         gCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAACCCGCTGCTTCGGAAGGCGC‑GGTTG‑GTTTG‑GAGgcc                                                                                                       >  7:183403/1‑134 (MQ=38)                           acggggCGGGGTTGCCTACTTTGTCCCTCACCGGTTTTCCCCTCAGCTTTTATTGCGGGGGTTTTTGGGGCTGCTTTGATGCCATTT‑CG‑C‑GGGTAAACCCGCTCCTACTAAAGCCGC‑TTTTG‑TTTTG‑TAGGggc‑g                                                                                                    <  4:141256/131‑1 (MQ=255)                             gCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAACCCGCGCCTACGAAAGCCGG‑GGATG‑GTTTG‑TAGGCGC‑ggg                                                                                                  >  2:242755/1‑135 (MQ=39)                               ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCGCCAAAGAAAGCCGC‑GGATT‑TTTTG‑TTTGCGC‑ggggt                                                                                                >  8:347239/1‑133 (MQ=38)                                    gggTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAACCCGCTGCTACGCAAGCCGC‑GTATG‑TTTTG‑TTGGCGC‑GGGGTTT‑ccc                                                                                          >  1:124103/1‑135 (MQ=38)                                              cTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCGACGAAAGCCGC‑GGATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGgc                                                                               >  7:94650/1‑135 (MQ=255)                                                 cTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGgccg                                                                             >  2:258240/1‑134 (MQ=255)                                                  tGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGGTAAACCGCGCCGACGAAAGGCGCGGTGTT‑TTTTG‑AGGGCG‑‑GGGTTTT‑CCCGCGCA‑TGGgccccc                                                                           >  6:277044/1‑135 (MQ=255)                                                    ccTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCCCGCCGACGAAAGCCGC‑GGATT‑TTTTT‑TTTGGGCGGGGTTTA‑CCGCCGAA‑TGGGCCCCCa                                                                          >  1:351684/1‑135 (MQ=255)                                                    ccTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAAACCCCGCCAAACCAAGCCGCGGTTGG‑TTTTT‑TGGGGCC‑GGGTTTA‑CCC‑CCAA‑TTGGCCCCCaa                                                                         >  2:118358/1‑135 (MQ=255)                                                      tGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAACCCCCGCCAAAGAAAGCCGC‑GTATG‑TTTTG‑TTGGCGC‑CGGGTTA‑CCCCCGAA‑ATGGCCGCCAAGc                                                                       >  5:106634/1‑135 (MQ=255)                                                      tGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGc                                                                       >  1:239045/1‑135 (MQ=255)                                                          cGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCCCGACGACGAAAGCCCC‑GTATG‑TTTTT‑TTTGCGC‑GGGGTTA‑GCCGCGAA‑TGGGGCCCCCAGCGGcc                                                                   >  4:245521/1‑135 (MQ=255)                                                           gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTTCTTTGCAGCCCATT‑AGCC‑GGTTAAAACCCGGCCAAAGAAAACCCG‑GAATG‑TTTTT‑TATGCCG‑GGGGTTA‑CACCCGAA‑TGGGCCCCCCAACGGcc                                                                   >  2:355207/1‑135 (MQ=255)                                                           gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCATCCCATT‑CG‑CGGGGTAAACAC‑CGCCGAACAAATCCCG‑GGATT‑TTTTGTTGGGCGG‑GGGGTTA‑GCCGGGAA‑GGGGCC‑CCCAGCGGccc                                                                  >  4:216028/1‑135 (MQ=255)                                                           gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAACCCGCGCCGACGAAAGCCGC‑GGATG‑TTTTG‑TTGGCGC‑GGGGTTT‑CCCGCGCA‑TTGGCCGCCAAGCGGccc                                                                  >  5:344028/1‑135 (MQ=38)                                                           gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCGCGCCTACGAAAGCCCC‑GGTAG‑TTTTG‑TATGCGC‑GGGTTTT‑CCCCCGCA‑TGGGCCCCCAACCGGgcc                                                                  >  6:239830/1‑135 (MQ=25)                                                           gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TATGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGcc                                                                   >  2:67536/1‑134 (MQ=255)                                                           gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GGATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGcc                                                                   >  7:264036/1‑134 (MQ=255)                                                           gAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCCCCCGCAGCCGC‑GGTAG‑GTTTG‑TTGGCGC‑TGGTTTT‑ACCGCGAA‑TGGGCCGCCCAGCGGgcc                                                                  >  8:84662/1‑135 (MQ=25)                                                               tttCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCGCCGACGAAAGCCGC‑GGTTG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGGCGCCCAGCGGCCCCaa                                                               >  5:85801/1‑134 (MQ=37)                                                               tttCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAAACCCCTCCTAAGAAAGCCGC‑GGATG‑TTTTG‑TATGCGC‑GGGTTTA‑ACCGCGAA‑ATGGCCGCCCACCCGcccc                                                                 >  5:108177/1‑132 (MQ=37)                                                                 tCCCTCAGCTAGTTTTCAGGGGGCTTTGGGGGCGGCTTTGCAGCCCATT‑CG‑C‑GGGAAAACCCGCTCCTACTAAACCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATg                                                             <  7:53124/134‑1 (MQ=25)                                                                 acccTCAGCGAGTTTTCAGTGCGCTTTGGGGGCGGCTTTGCAGCCAATT‑GG‑C‑GGCTAACCCCGCTCCTACGAAAGCCGG‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATg                                                             <  7:343097/133‑1 (MQ=37)                                                                  cccTCAGCTAGTATTTCGTGGGCTTTTGGGGCTGCTTTGCACCCAATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGa                                                            <  1:110418/134‑1 (MQ=38)                                                                   ccTCCGCGAGTATTGCGTGGGCTTTGGGGGCTGCTTTCCAGCCCATT‑CG‑C‑GGGAAAACCCGCTCCTACCAAAGCCGC‑GTATG‑TTTTG‑TCGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGaa                                                           <  2:77241/134‑1 (MQ=38)                                                                     tCAGCGAGTATTGCGTGGCCTTTTGGGGCTGCTTTCCTGCCCATT‑CG‑C‑GGGTAACCCCGCGCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCa                                                        <  1:109934/135‑1 (MQ=37)                                                                     tCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCTTT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTGG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCa                                                        <  6:354779/135‑1 (MQ=255)                                                                          gtgttttGAGGGTGTTTGTGGGGTGGTTTTGCTGCCCATT‑CG‑C‑GGTTAACCCCGCGCTACCGAAAGCCGC‑TTATG‑TTTTG‑TGGGCGC‑GGGTTTA‑CCCGCTAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑g                                                  <  6:56845/130‑1 (MQ=255)                                                                                    tGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCg                                       <  1:232909/135‑1 (MQ=255)                                                                                    tGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCg                                       <  7:291625/135‑1 (MQ=255)                                                                                    tGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCGCCTACCAACCCCGC‑GTATG‑TTTTG‑TCGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCg                                       <  6:296312/135‑1 (MQ=37)                                                                                    tGGGCTTTTGGGGCTGCTTCGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTCCTAAAGCGGC‑GTATG‑TTATG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCg                                       <  7:254176/135‑1 (MQ=38)                                                                                     gggCTTTTGGGGCTCGTTTCCCCCCCTTT‑CG‑C‑GGGTAACCCCGCGCCTACGAACGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGTTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCg                                       <  1:367341/134‑1 (MQ=255)                                                                                      ggCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCGCCTACGGAAGCCGC‑GTATG‑TTTTT‑TTGGCGC‑GGGGTTT‑CCCGCGAA‑TGGGCCGCCCAGCGGGCCCCAAGGATAAGAGG‑G‑AAATGGGCCGgc                                     >  6:69713/1‑135 (MQ=25)                                                                                       gCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGgc                                     <  2:257852/134‑1 (MQ=255)                                                                                         ttttGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCGCGGCGTCCGAAGCCGG‑GGATG‑TTTTT‑GTGGCGC‑GGGGTTT‑CCCGCGGA‑TTGGGCGCCACACGGGCCCAATGTATCAGAGC‑G‑CAAAGGGCCCGGGcc                                  >  4:199541/1‑135 (MQ=255)                                                                                         ttttGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAAACCCCGGCGAAGCAAGGCGC‑GGATG‑GTTTG‑TTTGCGC‑GGGTTTT‑ACCGCGAATTGGGCCCCCAA‑CGGCCCCAAAGAATAAGCGC‑GCAAATTGGCCgcccc                                   >  4:249592/1‑131 (MQ=255)                                                                                          tttGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTTAACCCGCGCCTACGGAAGCCGC‑GGATG‑GTTTG‑TAGGCGC‑GGGGGTT‑CCCGCGCA‑TGGGCCCCCCAGCGGCCCCCATGAAACAGCGC‑G‑AAATGGGCCGGCcccc                                 >  8:270188/1‑135 (MQ=25)                                                                                          tttGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCGCCGGCGCAAGCCGG‑GGTTG‑TTTTG‑TAGGCGC‑GGGGTTA‑CCCGCGTA‑TGGGGCGCCCAGCGGCCCCCATTCATAAGGGC‑G‑GAATGGGCCGGCGccc                                 >  1:28511/1‑135 (MQ=21)                                                                                          tttGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAAACCGCGGCGACGCAAGCCGC‑GGATG‑GTTTG‑TATGCGC‑GGGGTTT‑CCCGCGAA‑ATGGCCGCCCAGCGGGCCCCATGGAAAAGAGC‑G‑CAATGGGCCGCCGccc                                 >  6:107137/1‑135 (MQ=255)                                                                                          tttGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGGTAACCCCCGGCGGGGCAAGCCGG‑GGTTGTTTTTT‑TTGGGGG‑GGGTTTA‑CCGCGGAA‑TGGGCCCCCACGGGGCCCCAA‑GGATAAGGGCGG‑AAATGGGCC‑GCGccc                                 >  8:315917/1‑135 (MQ=255)                                                                                            tGGGCCTGCTTTGCAGCCCATG‑GG‑C‑GGGGAAACCCGCTCCTACTAACGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCt                                <  3:87166/134‑1 (MQ=255)                                                                                            gggggCTGATTCGCCCCCCATG‑CG‑C‑GGGTAAACCCGCGCCTACTAACGCCGC‑TTATT‑TTTTG‑TCGGCGC‑GGGTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCt                                <  4:99050/133‑1 (MQ=37)                                                                                             ggggCGGCGTTGCAGCCCACT‑CG‑C‑GGGCAAACCCGCTCCAACGAAAGCCGC‑GTATG‑TTTTG‑ACGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTg                               <  7:267800/134‑1 (MQ=38)                                                                                                 gtggtttCCAGCCCATT‑CG‑C‑GGGAAAACCCGCTCCTACTAAACCGGC‑GTTTG‑TTTTG‑TCGGGGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  1:17805/131‑1 (MQ=37)                                                                                                 ctgctTTGCAGCCCTTT‑CG‑C‑GTTTAAACCCGCTCCTACGAAAGCCGC‑GTTTG‑TTTTG‑TCGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  5:350077/135‑1 (MQ=39)                                                                                                 ctgctTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TCGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  5:361148/135‑1 (MQ=255)                                                                                                 ctgctTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  7:99440/135‑1 (MQ=255)                                                                                                 ctgctTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACCAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  7:228271/135‑1 (MQ=255)                                                                                                 ctgctTTGCACCCCATG‑CG‑C‑GGGTAACCCCGCTCCTACGAAAGCCGC‑GTATT‑TTTTG‑ACGCCGC‑GGGTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  7:320230/135‑1 (MQ=255)                                                                                                 ctgctTTCCAGCCCATT‑CG‑C‑GGGAAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  3:242232/135‑1 (MQ=255)                                                                                                 cggctTTCCAGCCATTT‑CG‑C‑GGGTAACCCCGCCCCTACCAACCCCGC‑TTAGG‑TTTTG‑TCGGCGC‑GGTTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAg                          <  5:130780/133‑1 (MQ=255)                                                                                                      tgccAGCCCATT‑CG‑C‑GGAAAACCCCGCTCCAACGAACGCCGC‑GATTC‑TTTTG‑ATGGCGC‑GGTTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATg                     <  7:34336/132‑1 (MQ=255)                                                                                                      gtGCAGCCCTTT‑CG‑C‑GGGTAACCCCGCTCCTACGAACCCCGC‑TTATG‑TTTTG‑TAGGGGG‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATg                     <  5:101873/134‑1 (MQ=38)                                                                                                       tGCAGCCCTTT‑CG‑C‑GGTTAAACCCTCTCATACTAAACCCGC‑TAATG‑TTTTG‑TCGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATTAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATg                     <  8:325640/134‑1 (MQ=37)                                                                                                       gcccgCCAATT‑CG‑C‑GGGAAAACCCGCTCCACCCAAACCCGC‑TTTTG‑TTTGG‑TCGGCGC‑GGGTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATg                     <  3:324487/130‑1 (MQ=255)                                                                                                       gcccccccTTT‑CG‑C‑GGTAACCCCCGCCCCTCCCAACCCGGC‑GTTTG‑TTTGG‑CCGGCG‑‑GGGTTTACCCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATg                     <  3:211205/129‑1 (MQ=255)                                                                                                        gCAGCCCATT‑CG‑C‑GGGTAAACCCGCGCCTACGAAAGCCGC‑GTATG‑TTTTG‑TCGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATg                     <  7:190436/133‑1 (MQ=255)                                                                                                        ccaccccATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATg                     <  2:293922/129‑1 (MQ=255)                                                                                                         ctgCCCATT‑CG‑C‑GGGTAAACCCGCTCCTCCCAACGCCGC‑GTATG‑TTGTG‑ACGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATggg                   <  8:246642/132‑1 (MQ=38)                                                                                                         cAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAACGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATggg                   <  1:21007/134‑1 (MQ=255)                                                                                                         cAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAACGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTAA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATggg                   <  7:105010/134‑1 (MQ=255)                                                                                                         cAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATggg                   <  8:94650/134‑1 (MQ=255)                                                                                                                 t‑CG‑C‑GGGTTAACCCGCGGCGTCGAAAGCCGC‑GGTTG‑TTTTG‑TTGGCGC‑GGGGTTA‑CCCGCGAA‑TGGGCCGCCCAGCGGCCCCCATGAATGAGCGC‑G‑AAATGGGCCGGCGCCCGGCAAAGGCCTGGGCCAGGGTAc          >  6:65785/1‑135 (MQ=255)                                                                                                                      c‑gGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAccgc       <  2:234088/135‑1 (MQ=255)                                                                                                                          gTAAACCCGCGCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGt    <  4:185080/135‑1 (MQ=255)                                                                                                                           aaaaCCCGCCCCTACGAACGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGTGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTc   <  6:283334/134‑1 (MQ=255)                                                                                                                            acaCCCGCTCATCCGAACCCCGC‑GTAGG‑GTATG‑ACGCCGC‑GGTTTAC‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTcc  <  8:65255/133‑1 (MQ=255)                                                                                                                            aaaCCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTcc  <  6:172902/135‑1 (MQ=255)                                                                                                                            aaaCCCCCACCCGCGAAAGCCGG‑GGAAG‑TGTTG‑GAGGCGC‑GGGGTTT‑CCGGCGCA‑TGGGCCGCCAAGCCGCCCCCATGGATCACAGC‑C‑AAATGGGGCGGCGCCCTGCAAAGGCAAGGGCCCTGGTAccgccgggc  >  8:294137/1‑132 (MQ=255)                                                                                                                                    |                                                                                                                                       GGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATT‑CG‑C‑GGGTAAACCCGCTCCTACGAAAGCCGC‑GTATG‑TTTTG‑TAGGCGC‑GGGTTTA‑CCCGCGAA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGC‑G‑AAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCC  >  ACB143/4844843‑4845097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑