Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,175,552	0	A	C	41.5%	30.1 / 12.5	41	N49T (AAC→ACC)	tadA	tRNA‑specific adenosine deaminase
Reads supporting (aligned to +/- strand):  ref base A (14/10);  new base C (2/15);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 3.50e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGTG  >  ACB143/1175432‑1175684                                                                                                                           |                                                                                                                                     gTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCACCCGGCCGATAATCGAc                                                                                                                      >  6:160119/1‑137 (MQ=255) gTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGAc                                                                                                                      >  4:439814/1‑137 (MQ=255)                 cATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAgcgcgc                                                                                                      >  3:151119/1‑137 (MQ=255)                     cgcCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGCTCATCGACAGCGACCCCAGCGCGCCTGc                                                                                                  >  1:208277/1‑137 (MQ=255)                                 gCCTGGGCCGCCGAGGGGGGGGGGTGGGGCGAGGTGCCGGTGGGGGGGGTGCTGGTCCA‑GCATGGG‑CTGGTGAGCGGCCTGGGTTTCACCCGGCCTATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGc                                                                                      <  8:223603/137‑1 (MQ=255)                                  ccTGGGCCGCCGGCGGGGGGGGGTGGGGCGCGGGGCCTGGGGGGGGGGGGCGGGTCCA‑G‑ATGGGCCTGGTGCGGGGCCTGGTTTTCACCGGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCg                                                                                     <  8:450243/137‑1 (MQ=255)                                   cTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCGGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGa                                                                                    >  8:260162/1‑137 (MQ=255)                                         cgccgAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCACCCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAggg                                                                              >  3:314250/1‑137 (MQ=255)                                         cgccgAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAggg                                                                              >  1:426127/1‑137 (MQ=255)                                              aaGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGggcggcc                                                                         >  7:450243/1‑136 (MQ=255)                                              aaGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCa                                                                         >  2:306417/1‑137 (MQ=255)                                                     ggcgTGGGGCGCGGGGCCTGTGGGGGGGGGGCGGGGGCA‑CCTTGGG‑CCGGGTAGCGGCCAGGGTTTCACCCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGt                                                                  <  7:262044/137‑1 (MQ=255)                                                       gggggggCGAGGGGCCTGGGGGGGGGGGGCGGGGCCA‑CCAGGGG‑CCGGTCCCCGGCCAGGGTTCCACCGGCCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCg                                                                <  2:116296/133‑1 (MQ=255)                                                                      ctggggggggggTGC‑GGGGCACGCAGGGG‑CCGGTCAGCGCCCTGGGTTCCACCCGGCCTACAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCgg                                                 <  3:48015/133‑1 (MQ=255)                                                                      ctgggggggCGGTGCTGGGGCA‑GCATGGG‑CAGGGGATCGGCCAGGGTTTCACCCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCgg                                                 <  6:496091/133‑1 (MQ=255)                                                                        gTGGGGGGGGTGCTGGTGCT‑GCTTGGG‑CAGGTGAGCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCt                                               <  5:163322/137‑1 (MQ=255)                                                                          gggggCGGTGCTGGTGGA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTAc                                             >  7:449204/1‑137 (MQ=255)                                                                              ggggTGCGGGGGCA‑GCATGGG‑CCGGTGAGCGCCCGGGGTTGCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCgg                                         <  4:314250/135‑1 (MQ=255)                                                                              gCGGTGCTGGTCCA‑GTATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCgg                                         <  6:466794/137‑1 (MQ=255)                                                                                  tGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGgcagc                                     >  4:235565/1‑137 (MQ=255)                                                                                          ca‑gctTGGG‑CAGGTGAGCGGCCTGGTTTTCACCCGGCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  1:44280/137‑1 (MQ=255)                                                                                          ca‑gcaTGGG‑CTGGTGATGGGCCTGGGTTTCACCCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  1:316576/137‑1 (MQ=255)                                                                                          ca‑gcaTGGG‑CTGGTCATCGGCCCGGTTTTCACCCGGCGGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  1:305551/137‑1 (MQ=255)                                                                                           ctgcttGGG‑CTGGTGAGCGGCCTGGGTTCCACCGGGCGTATAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  7:267279/132‑1 (MQ=255)                                                                                           ccgcaTGGC‑CAGGCGAGCGCCCAGGGTTTCACCCGGCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  8:288363/135‑1 (MQ=255)                                                                                           caccaTGGG‑CCGGTGAGCGGCCCGGGTTTCACCGGCCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  1:361493/134‑1 (MQ=255)                                                                                           atgcaTGGG‑CTGGTTTTCGGCCTGGGTTTAAACCGGCGTTTAATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  5:129040/135‑1 (MQ=255)                                                                                           acgctgggg‑CTGGTCAGCGGCGCGGGTTCAACCCGCCCTAACATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  3:32797/131‑1 (MQ=255)                                                                                           aagattGGG‑CTGTTCATCGGCCTGGGTTTAAACCGGCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  5:165563/132‑1 (MQ=255)                                                                                           aagaaTGGG‑CTGGTTATCGGCCAGGGTTTCAACGGGCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTa                             <  7:231347/133‑1 (MQ=255)                                                                                                tagg‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGa                        >  3:28325/3‑137 (MQ=255)                                                                                                      aGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTg                   >  4:162317/1‑137 (MQ=255)                                                                                                       ggTGATGGGCCAGGGTTTCAACCGGCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                  <  8:312493/137‑1 (MQ=255)                                                                                                       ggTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTgg                  >  3:204392/1‑137 (MQ=255)                                                                                                           aTCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAAcc              >  1:367974/1‑137 (MQ=255)                                                                                                                 ctggttttCACCCGGCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag        <  8:449204/132‑1 (MQ=255)                                                                                                                 cAGGGTTTCCCCCGGCCTAGCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTgcag        <  6:43716/137‑1 (MQ=255)                                                                                                                      tttCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgt   >  8:144335/1‑137 (MQ=255)                                                                                                                      tttCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgt   <  1:306417/137‑1 (MQ=255)                                                                                                                       ttCAACCGGCCTATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTTGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgtg  <  1:80320/137‑1 (MQ=255)                                                                                                                       ttCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCAtgtg  <  2:426127/137‑1 (MQ=255)                                                                                                                           |                                                                                                                                     GTCGCGATCAGGAATTCATGCGCCTGGCGCTGGCCTTGGCCGCCGAAGGCGCGGCGTTGGGCGAGGTGCCAGTGGGGGCGGTGCTGGTGCA‑GCATGGG‑CAGGTGATCGGCCAGGGTTTCAACCGGCCGATCATCGACAGCGACCCCAGCGCGCATGCCGAGATGGTGGCGATCAGGGCGGCAGCCAGGTCGGCCAGCAACTACCGGCTACCCGGCAGCACCCTGTATGTGACCCTGGAACCGTGCAGCATGTG  >  ACB143/1175432‑1175684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑