Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,626,0890TG40.0% 38.7 / 10.1 50V174G (GTG→GGG) hcf136_3Ycf48‑like protein
Reads supporting (aligned to +/- strand):  ref base T (14/16);  new base G (0/20);  total (14/36)
Fisher's exact test for biased strand distribution p-value = 2.16e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGC  >  ACB143/3625955‑3626219
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ggcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTgg                                                                                                                                  >  8:48035/1‑137 (MQ=255)
 gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGc                                                                                                                                 >  1:116819/1‑137 (MQ=255)
 gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGc                                                                                                                                 >  8:256876/1‑137 (MQ=255)
      cGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATgc                                                                                                                            >  6:162607/1‑137 (MQ=255)
        aGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATgcgc                                                                                                                          >  3:230827/1‑137 (MQ=255)
                  aGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCgaccacc                                                                                                                >  1:99185/1‑136 (MQ=255)
                              ccTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCt                                                                                                    >  5:301747/1‑137 (MQ=255)
                                 ggcggcggcGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGc                                                                                                 >  3:460965/1‑137 (MQ=255)
                                     gcggcgCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATTCGCACCGACGACGGCGGGCGCAGCTGGCCGTc                                                                                             >  2:194665/1‑137 (MQ=255)
                                     gcggcgCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTc                                                                                             >  1:110924/1‑137 (MQ=255)
                                        gcgcAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGga                                                                                          <  2:110924/137‑1 (MQ=255)
                                        gcgcAACGGCGGCGGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCGGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGga                                                                                          <  2:62731/137‑1 (MQ=255)
                                           cAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTGGGGGGC‑GACGGGGTGGCCAAGCGCCCCGACGCCCGGGGGGGCCGCGGGCAGTCGGGGAt                                                                                       >  3:4626/1‑137 (MQ=255)
                                             ccggcggcggGCCGTGGGCGCGGCCAAGCTGTTGTTGGCCCTTAATTTCCCCGATGCCGCCACCGGCGTGGGCGGGGGGGAC‑CAGGGGGTGCCCATGCGCCCCGCCGACGGCGGGCGCAGCTGGCAGTCGGAGATCg                                                                                     <  8:439333/136‑1 (MQ=255)
                                                       gcctcgggCGCGCACACGCCGTTGTTGGCCCTGAATTGACCCGTGGCCCGCAACGGCCTGGGCGGCGGTGCC‑TCCGGGGTGCCCATCCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  6:260944/131‑1 (MQ=255)
                                                       gccgcgggCGGGCACACGCGGTTGTTGGCCCTGAATTGCCCCGATCCCCCCACCGGCCTGGGCGTGGGGCCC‑TAGGGGGGGCCCTTGCCCACCCACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  8:20871/131‑1 (MQ=255)
                                                       gccgccGGGGCGGACACGCCGTGGTGGGCCCTTAATTGACCCGATGCCCGCACCGGCGGGGGGGGGGGTGCC‑TCCGGGGGGGCCAGGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  3:111968/137‑1 (MQ=255)
                                                       gccgccGGCGCGGACACGCGGTTGTGGGCCGTGAATTTCCCCGAGGCCCGCACCGGCCGGGGCGGCGGGGCC‑TCGGGGGGGGCCCGGCGCCCCGCGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  3:446703/137‑1 (MQ=255)
                                                       gCCGACGGCGCGTACACGCCGTTGTTGGCCCTGAATTTCACCGAGCCCCGCACCGGCGGGGGCGGCGGTGCC‑TACGGGGTGCCCATGCGCACCGCCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  4:95597/137‑1 (MQ=255)
                                                       gCCGACGGCGCGTACAAGCCGTGGTTGGCCCTTAATTTCACCGATGCCCGCAACGGCCGGGGCGTCGGGGCC‑TAGGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  7:479598/137‑1 (MQ=255)
                                                       gCCGACGGCGCGGACACGCCGTTGTTGGCCCTGAATTTCACCGAGGCCCGCACCGGCCTGGTCGGCGGGGCC‑TACGGGGGGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  2:323482/137‑1 (MQ=255)
                                                       gCCGACGGCGCGGACAAGCGGTTGTTGGCCCTTAATTTCACCCATGCCCCCAACGGCCGGGCCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACTACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  6:382872/137‑1 (MQ=255)
                                                       gCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCACCGGCCTGGTCGTCGGTGCG‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  6:476320/137‑1 (MQ=255)
                                                       gCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGGGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                           <  8:386203/137‑1 (MQ=255)
                                                             ggCGGGGACAAGCCGTTGTGGGCCCTGAATTTCACCGAGGCCCGCACCGGCCTGGTGGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg                                                                     <  4:460965/137‑1 (MQ=255)
                                                              gcgcGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCCCCGACGCCGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGCCTGCCGAACggg                                                                    >  3:289566/1‑137 (MQ=255)
                                                               ggcgGACACGCCGTTGTTGGCCCTGATTTTCACCGAGGCCCGCACCGGCCGGGGCGTCGGGGCC‑TCCGGGGGGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGc                                                                   <  7:96767/136‑1 (MQ=255)
                                                                 gggACAAGCCGTTGTGGGCCCTGAATTGCACCGAGGCCCGCACCGGCCTGGGCGTGGGGGCC‑TCCGGGGGGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGt                                                                 <  6:301747/136‑1 (MQ=255)
                                                                 cgtacacGCCGTTGTGGCCCCTGAATTTCCCCGAGCCCCGCACCGGCCGGGGGGGCGGGGCC‑TACGGGGGGGCCAGGCGCACCGAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGt                                                                 <  1:148549/134‑1 (MQ=255)
                                                                    actaGCCGTTGTTGGCCCTTTATTTCCCGGTGGCCCGCAAGGGCGTGGT‑GTTGGGGCCTTCCGTGGGGGATTTGCGCACCGTGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                              <  2:253293/134‑1 (MQ=255)
                                                                    aCAAGCGGTTGTGGGCCCGTAATTTCCCCGAGGCCCCCAACGGCCTGGGCGTGGGTGCC‑TCGGGGGGGGCCTTGCGCACCGAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                              <  7:229310/137‑1 (MQ=255)
                                                                    aCAAGCCGTTGTGGGCCCTGAATTTCCCCGATGCCCGCAACGGCCGGGGCGTCGGGGCC‑TCGGGGGGGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                              <  4:423917/137‑1 (MQ=255)
                                                                      aaGCCGTTGTTGGCCCTTAATTTCACCGATGCCCGCAACGGCGTGGTCGGGGGGGCC‑TACGGGGGGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACt                                                            <  4:293735/137‑1 (MQ=255)
                                                                           tttgttgGCCCTCAATTACACCGAGCCCCCCACCGGCGGGGGGGGGGGGGCC‑TCGGGGGGGCCCAGGCCCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc                                                       <  3:469063/136‑1 (MQ=255)
                                                                           gttgtgGGCCCTGAATTCCCCGCAAGCCCCCACCGGCTGGGCCGGCGGGGCC‑TCCGGGGGGCCCATGCCCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc                                                       <  2:187624/137‑1 (MQ=255)
                                                                                    ccTTAATTTCACCGATGCCCGCAACGGCCTGGGCGGCGGTGCC‑TCCGGGGGGGCCATGCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGccc                                              <  3:350065/137‑1 (MQ=255)
                                                                                                cGATCCCCGCACCGGCCTGGTGGGCGGGGCC‑TACGGGGTGGCCAGGCGCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAg                                  <  5:143199/137‑1 (MQ=255)
                                                                                                cGATCCCCCCAACGGCCTGGGCGGCGGTGCC‑TCCGGGGTGGCCAGGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAg                                  <  8:286041/137‑1 (MQ=255)
                                                                                                  ctGCCCGCACCGCCCGGGGCGGCGGGGCC‑TACGGGGGGGCCAGGCCCCCCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                                <  1:372691/136‑1 (MQ=255)
                                                                                                      ccGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCAGGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAcc                            <  6:461678/137‑1 (MQ=255)
                                                                                                                  ggccggcgGTGCC‑TACGGGGGGGCCATGCGCACCGACGACGGAGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCg                <  3:449594/131‑1 (MQ=255)
                                                                                                                  ggTCGTCGGGGCC‑TACGGGGTGGCCATGCGAACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCg                <  6:397341/137‑1 (MQ=255)
                                                                                                                   gtcggcGGGGCC‑TCCGGGGGGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGc               <  7:256876/137‑1 (MQ=255)
                                                                                                                       tcgGGGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGc           <  5:463032/137‑1 (MQ=255)
                                                                                                                        gggggCC‑TAGGGGGGGGCCAGGCGCACCGAGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCg          <  6:22603/133‑1 (MQ=255)
                                                                                                                          gTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGCCTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAg        >  5:414212/1‑137 (MQ=255)
                                                                                                                           tGCC‑TAGGGGGTGCCCATGCGAACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGc       <  7:146912/137‑1 (MQ=255)
                                                                                                                           ggCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGc       <  3:395083/136‑1 (MQ=255)
                                                                                                                           gccc‑TACGGGGGGCCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGc       <  1:382840/135‑1 (MQ=255)
                                                                                                                                 aCGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGc  >  3:225999/1‑137 (MQ=255)
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GGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCC‑TACGGGGTGGCCATGCGCACCGACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGC  >  ACB143/3625955‑3626219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑