| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | ACB143 | 5,096,422 | C→A | 29.0% | intergenic (+35/‑155) | CJ019_04792 → / → CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,096,422 | 0 | C | A | 29.0% | 21.4 / 3.6 | 33 | intergenic (+35/‑155) | CJ019_04792/CJ019_04793 | Putative pterin‑4‑alpha‑carbinolaminedehydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (8/12); new base A (5/2); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCA > ACB143/5096287‑5096556 | tGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGACGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTc < 3:249373/136‑1 (MQ=255) cTGTTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTAATCATGTGTGCAGGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATg < 4:235263/135‑1 (MQ=255) tggtggAGCCACTCGATCAAAGGCCTGCACCGCAGCGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTGATggg > 1:124062/1‑136 (MQ=255) tggtggAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACTGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTCATtgg > 1:298006/1‑136 (MQ=255) tgttgAGCCCCTCGAACAAATGCCAGCACCGCAACGACGTCCTCCTGTGTGCACGCACTGACAATGTTGCGGTAACGGCTGAAGGCCGGAATTAAACACCATAATGAATGGGCCAGAGGCCCCTTTTTTCATgggg < 4:208705/132‑1 (MQ=255) ggtggAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGATTTTTAATgggg > 6:51797/1‑136 (MQ=255) ggAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTAATGGGGCa > 2:9586/1‑135 (MQ=255) atccACTCGCTCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGTAACGGCTGCAGGCCGGAAGTAAACACCAGAAGGTAGGGGCCAGAGGCCCCTTTTTTCATGGGGCatat < 3:99421/134‑1 (MQ=255) gCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCg < 4:307242/136‑1 (MQ=255) ccTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTAGCCCGc > 1:267696/1‑136 (MQ=255) aCCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCCTGGGGCATATGTGCGCTGGTCGCCCGCAAA‑Ac > 6:76147/1‑136 (MQ=255) ccGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCCTTTTTTATTGTGCATATTTTCGCTGTTTCGCCGCAAA‑Acc > 6:193082/1‑136 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTAATTGGGCATATTTTCGCTGTTAGCCCGGAAA‑Acacccaa > 5:247409/1‑129 (MQ=255) cGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACCGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATTGGGCATATGTGCGCTCTTAGCCCCGAAA‑ACCGACaa > 6:210032/1‑135 (MQ=255) cacgcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTGATGGGGGCTATTTTCGGGGTTCGCCCGCCAA‑ACCCCCAAACGACCGGGACAAAAAc > 2:300895/1‑135 (MQ=255) gcacTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGTTTTTTAAAGGGGGATATTTGCGCTGTTCGACCGCAAA‑ACCCACAAACCCCCCGGATAAAAAACaa > 8:273224/1‑135 (MQ=255) gACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAAATGt > 8:289826/1‑135 (MQ=255) gACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCGTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACAGTc > 8:43678/1‑136 (MQ=255) cAAGGGGGCGTAAACGGCGGACGGCCGTAAGTAAACACCAGAAGGACGGGGCCAGAGGCCCCTTTTTGCATGGGGCTTATGTGCGCGGTGCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCAt < 6:115496/136‑1 (MQ=255) cggTGGCGGAATCGGCTGAAGGCCGGAAGTAACCACCAGAAGGACGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTACGCCCGCAAA‑ACCCACAAACGACCGGGAAAAAAACCAAACTGTCATcc < 5:254969/135‑1 (MQ=255) aGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATAAGTGCGCTGTTAGCCCGCAAA‑ACCGACAAACGCCCCGGAAAAAAACCAAACTGGCATc > 1:262274/1‑135 (MQ=255) cTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCGGTTTTTGATTGGGCAGATTTGGGCGGTTCGCCCGCCAA‑AACGACAAACGCCCGGGGAAAAAACCAAACGGGCCTACAGACGTGTGTTTCCt > 3:223886/1‑136 (MQ=255) taaGTAACCACCAGAAGCAAGGGGCCAGAGGACCCTTTTTTCATGGGGCATATGGGCTCTGTTCGCCCGCAAA‑ACCGACAAACGACCTGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCtt < 5:49786/135‑1 (MQ=255) aaGAAAACACAAGAATTAAGTGTCCATAGTCCCCTTTTTTAATGGGTCATATTTGAGCTTTTAGAACGCAAA‑ACAGAAAAACGAACGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCtt < 5:45048/135‑1 (MQ=255) acCAGAAGGACGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCt < 6:279804/136‑1 (MQ=255) ggagtgggCCCCAGGCCCATTTTTAATTGGGGCATATGTGCGCGGTGCCCCCCCAAA‑ACCGACACACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAAcg < 1:9586/131‑1 (MQ=255) gcagggggCCCGCGGCCCGTTTTTTCTTGGGGCATTTGGGCGCTGTCCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAAcg < 5:76147/132‑1 (MQ=255) ggCCAGAGGCCCGGTTTTTTATTGGGGGGATGTTCGCTGGTCGGCCGCCAA‑ACCGCCCAACGACCGGGAAAAAAACCAAACAGTGATCCAGACTTTTGTTTCCTGCCCCAGCTGTTTTTAGCTACAAACCCGccag > 2:309481/1‑134 (MQ=255) cccctcgCCCCTTTTTTTATTGGGCAAATGGGGCGGGTTCCCCCCCAAACCCCCCCCACCGCCCGGCAAAAACACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTgg < 6:200649/130‑1 (MQ=255) tttttCATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACtt < 7:206218/132‑1 (MQ=255) tttttCATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACtt > 8:206218/1‑132 (MQ=255) tttttCATGGGCCATAGGTGCGCTGTTCGCCCGAAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTtc < 5:110084/135‑1 (MQ=255) tttttAAAGGGGCATATGTGCGCTGTTACCCCGCAAA‑ACCCACAAACGACCGGGAAAAAAACCAAACTGTCATCAAGACATGTGTATACTGCCCCGGCTTTTTGAATCTACAAACCCGCCAGGCGCAGACTTTtca > 3:243603/1‑136 (MQ=255) tttCATGGGGCTATTGTCCGCTGACCCCCCCAACA‑ACCGCAATACGACCTGGACAAAAACCAAACTGTCATCCAGAATTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTtcac < 2:220307/135‑1 (MQ=255) cATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTtcactca < 6:120280/135‑1 (MQ=255) | TGACCTGGTGGAGCCACTCGATCAAAGGCCTGCACCGCAACGACTTCATCATGTGTGCACGCACTGACAAGGTGGCGGAAACGGCTGAAGGCCGGAAGTAAACACCAGAAGGAAGGGGCCAGAGGCCCCTTTTTTCATGGGGCATATGTGCGCTGTTCGCCCGCAAA‑ACCGACAAACGACCGGGAAAAAAACCAAACTGTCATCCAGACTTGTGTATCCTGCCCGAGCTTTTTGAAGCTACAAACGCGCCTGGCGCAGACTTTTCACTCA > ACB143/5096287‑5096556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |