| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 120,983 | (T)9→8 | intergenic (+280/+92) | polA → / ← engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 120,975 | 0 | T | . | 98.0% | 214.4 / ‑3.1 | 49 | intergenic (+272/+100) | polA/engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (34/14); total (34/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCAGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTT > KT2440/120841‑121100 | cTCAGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGt > 3:256589/1‑135 (MQ=255) tAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGtttttttt < 4:16219/135‑1 (MQ=255) aaCTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttgc > 7:140692/1‑134 (MQ=255) tGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCg > 8:95228/1‑136 (MQ=255) aTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCgg < 6:271572/134‑1 (MQ=255) cTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAAttt > 4:182107/1‑135 (MQ=255) cTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCAGACGAAGTCCGGGG‑TTTTTTTTGCCCGGGAttt > 5:225393/1‑135 (MQ=255) gAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACACCGCCCTAGCGGTCCGGACTTGGACGCCGAACTTCCCCATCCCCATACGAAGTCCGGGG‑TTTTTTTTGGCGGGAATTTGGCgg > 1:149884/1‑135 (MQ=255) aTGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTTCCGGGAGCGGGTTTtc > 8:292955/1‑136 (MQ=255) aTGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGttt < 3:143379/134‑1 (MQ=255) gCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTtct < 4:290355/135‑1 (MQ=255) tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCACGCGa > 5:274938/1‑135 (MQ=255) aTGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCAAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGATTTTTCCGGGAGCGGGTTTTCTCGCGaa > 5:43275/1‑135 (MQ=255) tgttgtGTTAAGTGTTGTCAGATATCTGGACCCCGCCCTAGCGGTCCTGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 7:46623/135‑1 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 7:95228/135‑1 (MQ=255) gttgtGTTAAGTGGTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 2:30501/134‑1 (MQ=255) tgtgTTATGTGTTGGCAGATTTCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGTGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc < 3:113847/134‑1 (MQ=255) tgtgTTAAGTGTGGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCg < 5:83988/135‑1 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 1:138685/127‑1 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt > 2:138685/1‑127 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt > 3:268026/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 8:49301/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACACCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCAGGAATTTGCCGGGAGCTGGTTTTCTCGCGAATGCGATGta > 3:276256/1‑134 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa > 5:153580/1‑135 (MQ=255) cTGGACCCCTCCCTGGCGGTCGGGAGGTGGACCCCGAAAGTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCTGAATTTGCCGGGTGCGGTTTTTCTCGCGAATGCTATGTCGAATACACCGCCGCAt < 4:163319/134‑1 (MQ=255) cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCCTACGAAGTCCGGGG‑TTTGTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACcgccgccat > 6:22548/1‑132 (MQ=255) cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAtt > 3:170718/1‑135 (MQ=255) cTGGACCCCGCCCTAGAGGTCCGGACTTGTACCCCGAACTTCCCCCTCACCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATTCTATGTCGAATACACCGCCGCAt < 6:119765/134‑1 (MQ=255) cccGCCCTAGCGGTCCGGACTTGGACCCCGCACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGACTTTGCCGGGAGCGGGTATTCTCGCGAAGGCGATGTCGAATACACCGCCGCATTCGCgg > 1:242107/1‑134 (MQ=255) ccGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGt > 8:2828/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGCATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc > 7:20045/1‑136 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACCCCGCCCCATTCGCGGGTAAAc > 1:127056/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGt < 1:97467/131‑1 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGt > 2:97467/1‑131 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTTAAc > 2:42611/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc > 7:233596/1‑136 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGACCGGGG‑TGTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTATAGCGAATGCTATGTAGAAAAAACCCCCCCATTCGCGGGTAAAc > 2:138249/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCAAACGAAGTCCGGGG‑GTTTTTTTGCCCGGCATTTGCCGGGAGCGGGTTTTCTCCCGACAGCGGTGTCGAGTAAACCGCCGCAGTCGCggggtacc > 1:163787/1‑130 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTACCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCCTTCGCGGGTAAAc > 2:36231/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACACCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGGACGGAATTTGCCGGGAGAGGGTTTTCTCGCGAATTGTATGTCGAATACACCGCCGCATTCGAGGGTAAGcc > 7:132656/1‑136 (MQ=255) tAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAccc > 4:165554/1‑135 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt > 5:34299/1‑86 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 6:34299/86‑1 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcgc > 7:170702/1‑135 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg > 4:157581/1‑134 (MQ=255) ccccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAACGCCGCAGTCGCGGGGAACCCCGGGTCAGGCGCAAACCa > 1:246742/1‑134 (MQ=255) cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATCCACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAAcccc > 5:133848/1‑132 (MQ=255) ttCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCACACCACTAAACGAc > 1:129646/1‑135 (MQ=255) ccaaTACGACGGCC‑GGGTTTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc < 6:153580/132‑1 (MQ=38) cATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCtt > 3:265591/1‑135 (MQ=255) cGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCCGGTCAGGCGCAACCCACTAAACGACCGGCTTGTCTTCCAg > 2:219531/1‑135 (MQ=255) gacgTCCGGGG‑TTTTTTTTGCCCGTAATTTGCGGGTAGCGGTTTTGCTCGCGATTGCGATGTCGATTCCACCGCCGCATTCGCGGGTAAACCGGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGtt < 8:55158/133‑1 (MQ=255) | CTCAGAGTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTT > KT2440/120841‑121100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |