| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 3,061,793 | G→A | S592N (AGC→AAC) | qedA_2 → | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,061,793 | 0 | G | A | 94.4% | 57.1 / NA | 18 | S592N (AGC→AAC) | qedA_2 | Quinoprotein alcohol dehydrogenase (cytochromec) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (12/5); minor base C (1/0); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGG > KT2440/3061660‑3061909 | gTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAATGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaac‑g < 8:151700/136‑1 (MQ=255) gTAGTCTCCGGCGGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGc < 7:114219/136‑1 (MQ=255) tAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAg > 4:216639/1‑134 (MQ=255) tcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAAc > 7:256475/1‑136 (MQ=255) cggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAACC‑GTCAAGCCATAACGCCTGCCTACt > 7:48263/1‑136 (MQ=255) cgAAGTGGCCAAACGGGTCAAGGACTTCAACAAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCACACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAACGGt > 1:208602/1‑135 (MQ=255) aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATaa > 1:37051/1‑97 (MQ=255) aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATaa < 2:37051/97‑1 (MQ=255) aGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTAGCTGCCGCTGATCGGTGGc > 8:263595/1‑135 (MQ=255) gCCAAACGGGTCATGGCGTTCAACCAGGGCGGCAGGCTCTGGACCTTCAAGTTGCCCACGCAGTTGCAG‑AAACCGCAAACGGACAACCCATAACGCGTGCCTACTGCCGCTTGCGGCCGCTTATCGTTGGCAAGCg < 8:100393/136‑1 (MQ=255) ccAAAGGGGTCAAGGACTTCAACCCGGGGGGAATGCGCTGGACATACAAGTTGCCCAAGCAGTGGCAGCAAACGGAAAAC‑GTCAAGCCATACCGCCTGCCTACTGCCGCTTGCTGCCGCTGAGGGGGGGAAAGCg < 4:88688/135‑1 (MQ=255) aGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATAGGTGGCAAGCGGCCCTACTAcc > 4:10642/1‑134 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACg > 4:79372/1‑133 (MQ=255) aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCCCTTATAGGTTGAAAGCGGCCCGACTACCAAATTACgatc > 7:282710/1‑134 (MQ=255) ggACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATg > 8:153927/1‑136 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGCTCGGTGGCAAGCGGCCCGACGACCAAATGACGAGAGCCCCGCGGCCCACGATGCATTCGcc > 1:159879/1‑135 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGccc > 8:66526/1‑136 (MQ=255) aGTTGCCCAAGCAGTTGCAGCAAACGGCAAAC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcc > 1:198398/1‑135 (MQ=255) aGTTGCACCAACCGGCAAAC‑GTCAACCCAAAACCCCTGCCTCCTGCCGCTGGCTGCCGCTGAGCGGTGGCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCgggg < 2:91107/135‑1 (MQ=255) | GTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGC‑GTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGG > KT2440/3061660‑3061909 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |