Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	399,292	A→C	34.9%	intergenic (‑186/+122)	CJ019_00365 ← / ← aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	399,292	0	A	C	34.9%	34.8 / 4.6	43	intergenic (‑186/+122)	CJ019_00365/aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand):  ref base A (15/9);  new base C (7/8);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCA  >  KT2440/399169‑399421                                                                                                                              |                                                                                                                                  gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTCTGCGGCCTTGTGTCGCG‑AAAGG‑CGGGCGAAGccc                                                                                                                        >  5:50750/1‑135 (MQ=255)  atgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGccc                                                                                                                        <  8:299690/134‑1 (MQ=255)  atgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGccc                                                                                                                        <  1:95540/134‑1 (MQ=255)      cccGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAATG‑ACGGCGAAGCCCTCCCa                                                                                                                   >  2:49419/1‑135 (MQ=255)        cGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑GAAGG‑CGGGCGCAGCCCGCCCAGc                                                                                                                 >  1:99840/1‑135 (MQ=255)                 gCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑CGGGCGAAGCCCTCCCCGCTTTTTCCAg                                                                                                        >  8:220718/1‑135 (MQ=255)                  cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑AGGGCGGAGCCCGCCCCGGGTTTTCCAgg                                                                                                       >  2:194061/1‑135 (MQ=255)                  cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAg                                                                                                        >  8:214739/1‑134 (MQ=255)                  cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCGCCCAGCTTTTTcccg                                                                                                        >  2:226052/1‑132 (MQ=255)                  cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATATTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGCAGCCCTGCCCGCTTTTTcccg                                                                                                        >  6:138673/1‑132 (MQ=255)                    aGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCCCCTTTTTCCAGGCa                                                                                                     >  3:280764/1‑135 (MQ=255)                                      tgtgGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATc                                                                                  >  4:287672/1‑136 (MQ=255)                                         ggACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGGCTTGTGTGGCGCAAAGG‑CGGCCG‑AGCCCGCGCCGCCTTTTTAACCCAAAAAAAACCCCCGCCacaaga                                                                                >  1:346983/1‑130 (MQ=255)                                           aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCg                                                                              >  5:97288/1‑135 (MQ=255)                                           aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGAGGCCTTGGGTCGCG‑AAAGG‑AGGGCGACGCCCGCCCAGCTTTTTCCAGGCAAAAAAAAGACCAGCCAATCGACg                                                                              >  2:318138/1‑135 (MQ=255)                                            ccggTTAGCGGCTGACGTGGCCGATTCTGCTGTTGGGCGGCAGATTTTGGGGCCGGTTTGCGGCCTTGTGGCGCC‑ACAGG‑CGGGCGAAGCCCTCCCAGCTTTTTCCAGGAAAAAAAAAGCCCCGCCAATCGCCgg                                                                             <  7:214739/133‑1 (MQ=255)                                            cAGGTTATGGGGTGCCGGGGCCGATTCTGCAGTTGGGCTGGATATTTTGGGGCGGGTTTGCGGCCTTGTGTCGCT‑AAAGC‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGAAAAAAAAAGCCCCGCCAATCGACgg                                                                             <  8:95584/135‑1 (MQ=255)                                                ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGGGTTGGGGGCTTGTGGGGCG‑CAAGGCGGGGGGCGGCCCGCCCGCGTTTTGGCCGGAAAAAAAAACCCCCCCAAAGCGACggggg                                                                          >  6:16382/1‑134 (MQ=255)                                                  aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑CGGGCGCAGCCCTCCCCGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCGGGGGTGa                                                                       >  1:221470/1‑135 (MQ=255)                                                     ggCTGAGGTGCCGTATTGTGCTTTTGGGGTGGATTTTTTGGGGCCGCTTGGCGGCGTTGGGGCGCG‑ACCGG‑CGGGCTACGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAgg                                                                     <  2:70848/134‑1 (MQ=255)                                                       cTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑AGGGCGAAGCCCTCCCAGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTAc                                                                  >  4:43508/1‑135 (MQ=255)                                                            gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑CGGGCGAAGCCCTCCCCGCGTTTTCCAGGCCAAAAAAAGCCCCGCCAATCGACGGGGGTGAGGGACGAGCgg                                                            >  3:57243/1‑135 (MQ=255)                                                            gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑CGGGCGCAGCCCGCCCCGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCGGGGGTGAGGTACGAGCg                                                             >  1:70848/1‑135 (MQ=255)                                                            gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCg                                                             >  8:370604/1‑135 (MQ=255)                                                             tGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTATCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTg                                                           >  3:314299/1‑136 (MQ=255)                                                                 gtaTTCTGCATTTTGGCTGCAGATTTCGGGGCCGCTGTGCGGCGTTGGGTCGGG‑ACCGG‑TGGGCTACGCCGCCCCAGCTTTTACGAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAg                                                       <  4:225783/134‑1 (MQ=255)                                                                                ggtGGATATTTTGGGGCCGCTTTGCGGCGTTGTATCGCT‑AATGG‑CGGGCGAAGCCCTCCAAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTAc                                         <  4:314299/133‑1 (MQ=255)                                                                                    ttttttttGGGGCCGCTTGGGGGCTTTTGGTCGCT‑AACGC‑TGGGCCACGCCCGCCCAGCTTTTTCCAGCAAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGcc                                     <  5:61058/131‑1 (MQ=255)                                                                                      gaTTTTGGGGCGGCTTTGCGGCCTGGGGGCGCG‑ACAGG‑AGGCCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGcccc                                   <  8:174391/135‑1 (MQ=255)                                                                                        ttttGGGGCCGCTTGGCGGCCTGGGGGCGCG‑AACGG‑CGGGCGCCGCCCGCCCAGCTTGTGCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGGGGCAGCTCGAAAAAGGTACTGCCCCTc                                 <  7:130424/135‑1 (MQ=255)                                                                                                 cGCTTTGCGGCCGTGTGTCGCG‑AACGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                       <  4:80591/136‑1 (MQ=255)                                                                                                  gCTTTGCGGCCTTGGGGCGCG‑AACGG‑AGGGCGAAGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                       <  6:86481/135‑1 (MQ=255)                                                                                                       gCGGCCTGGTGGCGCG‑AACGG‑CGGGCGAAGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                 <  4:57243/136‑1 (MQ=255)                                                                                                        cGGCCTTGTGGCGGT‑AATGG‑AGGGCGACGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                  <  8:285207/134‑1 (MQ=255)                                                                                                        cGGCCTTGGGGCGCG‑AACGG‑CGGGCGACGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                  <  7:22340/134‑1 (MQ=255)                                                                                                        cGGCCGTGTGGCGCC‑AACGG‑CGGGCGACCCCCGCCCAGCTTTTTCCCGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                  <  7:186891/134‑1 (MQ=255)                                                                                                             tgggggcgcg‑AACGG‑GGGGGCAACCCCGCCCAGCTTTTTCCTGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTATTGCCCCTCGCAAGGAGGGGCAGTGTGCTg            <  7:244940/129‑1 (MQ=255)                                                                                                               gtggCGCG‑ACAGG‑CGGGCTAAGCCCTCCCAGCTTTTTCCAGCAAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGc          <  3:118157/135‑1 (MQ=255)                                                                                                                tgtCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGa                                                  <  8:23106/94‑1 (MQ=255)                                                                                                                tgtCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGa                                                  >  7:23106/1‑94 (MQ=255)                                                                                                                 gtCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCtt        >  8:62471/1‑135 (MQ=255)                                                                                                                        aaaCG‑AGGGCCAAGCCCGCCCAGCTTTTTCCCGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGGGGCAGCTAGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca  <  7:43027/135‑1 (MQ=255)                                                                                                                         aaGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca  >  8:334487/1‑134 (MQ=255)                                                                                                                              |                                                                                                                                  GATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCA  >  KT2440/399169‑399421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑