| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,809,389 | C→A | 45.6% | intergenic (+38/+53) | ftsN → / ← CJ019_05436 | Cell division protein FtsN/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,809,389 | 0 | C | A | 45.6% | 8.0 / 2.8 | 20 | intergenic (+38/+53) | ftsN/CJ019_05436 | Cell division protein FtsN/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (5/5); new base A (2/6); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAG‑CATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCCCACC > KT2440/5809256‑5809511 | ttCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCcttctt < 4:197494/136‑1 (MQ=255) tCAGCAACCGCGAGCAGTTGACGGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCcttctt < 2:272648/135‑1 (MQ=255) tCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCcttctt < 5:153436/135‑1 (MQ=255) aaCCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGGTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTatat < 1:60941/135‑1 (MQ=255) cAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAG‑CAtt < 3:248731/136‑1 (MQ=255) gagaGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGAAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAG‑CATTGCCCg < 3:23861/133‑1 (MQ=255) cAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAG‑CATTGCCCg < 6:289089/134‑1 (MQ=255) agacagcaGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAG‑CATTGCCCGTGa < 7:361526/129‑1 (MQ=255) cagcTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTTCCGCAGCGATGCGCCAAGGCCATAGTTATATTGACAG‑CATTGCCCGTGAAGGGGCTGTaaa > 7:89460/1‑135 (MQ=255) tggGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTTCCGCCGCGATGCGACAAGGCCATCTTTTTATTGGCAG‑CATTGCCCGTGAAGGGGCTGTAAagcctgtct > 8:361526/1‑129 (MQ=255) gggCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGCTGCGACAAGGCCTTCTTTATATGGACAG‑CATTGCCCGTGCAGGGGCTGTAAAGCAGCcccccc > 3:150822/1‑136 (MQ=255) ccGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCGTTGCGCAGCGCGTCGCCAAGGGCGGGTTTTTTTGGCCAGCCATGGCCCGGGAGGGGGGGGT‑AAGCCGCCCCGCcccc > 3:22533/1‑136 (MQ=255) tCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTTCCGCAGCGATGCGACAAGGCCTTCGTTATATGGACAG‑CATAGCCCGTGCAGGGGCTGTAAAGCAGCCCCCCCCCCCTccg > 7:186301/1‑133 (MQ=255) aaCCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCGTTATATGGACAG‑CATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGGCCgccccg > 8:275692/1‑135 (MQ=255) aaCCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCATTCCGCAGCGCTTCGACAACGGCGTATTTATATTGACAG‑CAATTCCCGTGAACGGGCTGTAAAGCAGCCCCCCCCCCCGCAgccccc > 7:92861/1‑134 (MQ=255) tGTTGCTGCAACAGCGGCAGACCCGTCACTAACGAAGAAGGCCGTGCCGCAGCGATGCCAAAAGGCCTTATTTATATGGACAT‑CATTGCCCGTGAAGGGGCTTTAAACCACCCCCGCCACCGTCAgccccgcccc < 8:92861/135‑1 (MQ=255) gTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCCGCGCTGCGCCAAGGCCGTCTTTATATGGACCG‑CATTGCCCGTGAAGGGGCGGTATAGCAGCCCCGCCACCGTCCGccccgccccg > 7:194902/1‑135 (MQ=255) actcagaggGCCTTGCCGCTGCGAGCCCACCTGGCTTTATTTATAGGCACCC‑CATTGCCCGTGCGGGGGCTTAAAAGCCCCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACg < 7:145390/131‑1 (MQ=255) aCGAACACGGCCTTGCCGCAGCGAGGCGACAAGGCCTTTTTTATATGGACAC‑CTTTGCCCGTGACGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACg < 7:348387/135‑1 (MQ=255) ctaagaagGCCTTGCCGCAGCGAGGCGACACGGCCTTATTTATATGGACAG‑CATGGCCCGGGAAGGGGCTTTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTc < 4:150822/134‑1 (MQ=255) acTTGCCGCAGCGATGCTACACGCCCTTATTTATATGCCCAG‑CTTGCCCCTTGATGGGGCTGTAACGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCa < 3:144178/135‑1 (MQ=255) ttGCCGCAGCGAGGCGACAAGGCATTATTTATATGCACAG‑CATGGCCGGTCAAGGGGCTTAAAACCCGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCAt < 6:134527/135‑1 (MQ=255) gACATGGCTTTATTTATATGGACAG‑CATTGCCGGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCccacc < 1:36504/134‑1 (MQ=255) | TTCAGCAACCGCGAGCAGTTGACAGGGGCACAGAAGCAGCTGGCTGGGGCCGGGTTCAGCAACCTGTTGCTGCAACAGCGGCAGACCCGTCAGTAACGAAGAAGGCCTTGCCGCAGCGATGCGACAAGGCCTTCTTTATATGGACAG‑CATTGCCCGTGAAGGGGCTGTAAAGCAGCCCCGCCACCGTCAGCCCCGCCCCGCATTCCTTTCGGACACCTGGCTGTTCAACGTCCAGAAGTCATACAGCACCCCCACC > KT2440/5809256‑5809511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |