Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	399,304	T→G	37.2%	intergenic (‑198/+110)	CJ019_00365 ← / ← aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	399,304	0	T	G	37.2%	23.6 / 3.0	42	intergenic (‑198/+110)	CJ019_00365/aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand):  ref base T (15/10);  new base G (7/9);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAGCATGG  >  KT2440/399174‑399427                                                                                                                                     |                                                                                                                            cccGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAATG‑ACGGCGAAGCCCTCCCa                                                                                                                         >  2:49419/1‑135 (MQ=255)   cGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑GAAGG‑CGGGCGCAGCCCGCCCAGc                                                                                                                       >  1:99840/1‑135 (MQ=255)            gCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑CGGGCGAAGCCCTCCCCGCTTTTTCCAg                                                                                                              >  8:220718/1‑135 (MQ=255)             cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑AGGGCGGAGCCCGCCCCGGGTTTTCCAgg                                                                                                             >  2:194061/1‑135 (MQ=255)             cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAg                                                                                                              >  8:214739/1‑134 (MQ=255)             cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCGCCCAGCTTTTTcccg                                                                                                              >  2:226052/1‑132 (MQ=255)             cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATATTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGCAGCCCTGCCCGCTTTTTcccg                                                                                                              >  6:138673/1‑132 (MQ=255)               aGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCCCCTTTTTCCAGGCa                                                                                                           >  3:280764/1‑135 (MQ=255)                                 tgtgGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATc                                                                                        >  4:287672/1‑136 (MQ=255)                                    ggACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGGCTTGTGTGGCGCAAAGG‑CGGCCG‑AGCCCGCGCCGCCTTTTTAACCCAAAAAAAACCCCCGCCacaaga                                                                                      >  1:346983/1‑130 (MQ=255)                                      aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCg                                                                                    >  5:97288/1‑135 (MQ=255)                                      aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGAGGCCTTGGGTCGCG‑AAAGG‑AGGGCGACGCCCGCCCAGCTTTTTCCAGGCAAAAAAAAGACCAGCCAATCGACg                                                                                    >  2:318138/1‑135 (MQ=255)                                       ccggTTAGCGGCTGACGTGGCCGATTCTGCTGTTGGGCGGCAGATTTTGGGGCCGGTTTGCGGCCTTGTGGCGCC‑ACAGG‑CGGGCGAAGCCCTCCCAGCTTTTTCCAGGAAAAAAAAAGCCCCGCCAATCGCCgg                                                                                   <  7:214739/133‑1 (MQ=255)                                       cAGGTTATGGGGTGCCGGGGCCGATTCTGCAGTTGGGCTGGATATTTTGGGGCGGGTTTGCGGCCTTGTGTCGCT‑AAAGC‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGAAAAAAAAAGCCCCGCCAATCGACgg                                                                                   <  8:95584/135‑1 (MQ=255)                                           ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGGGTTGGGGGCTTGTGGGGCG‑CAAGGCGGGGGGCGGCCCGCCCGCGTTTTGGCCGGAAAAAAAAACCCCCCCAAAGCGACggggg                                                                                >  6:16382/1‑134 (MQ=255)                                             aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑CGGGCGCAGCCCTCCCCGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCGGGGGTGa                                                                             >  1:221470/1‑135 (MQ=255)                                                ggCTGAGGTGCCGTATTGTGCTTTTGGGGTGGATTTTTTGGGGCCGCTTGGCGGCGTTGGGGCGCG‑ACCGG‑CGGGCTACGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAgg                                                                           <  2:70848/134‑1 (MQ=255)                                                  cTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑AGGGCGAAGCCCTCCCAGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTAc                                                                        >  4:43508/1‑135 (MQ=255)                                                       gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑CAAGG‑CGGGCGAAGCCCTCCCCGCGTTTTCCAGGCCAAAAAAAGCCCCGCCAATCGACGGGGGTGAGGGACGAGCgg                                                                  >  3:57243/1‑135 (MQ=255)                                                       gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑CGGGCGCAGCCCGCCCCGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGCCGGGGGTGAGGTACGAGCg                                                                   >  1:70848/1‑135 (MQ=255)                                                       gTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCGTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCg                                                                   >  8:370604/1‑135 (MQ=255)                                                        tGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTATCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTg                                                                 >  3:314299/1‑136 (MQ=255)                                                            gtaTTCTGCATTTTGGCTGCAGATTTCGGGGCCGCTGTGCGGCGTTGGGTCGGG‑ACCGG‑TGGGCTACGCCGCCCCAGCTTTTACGAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAg                                                             <  4:225783/134‑1 (MQ=255)                                                                           ggtGGATATTTTGGGGCCGCTTTGCGGCGTTGTATCGCT‑AATGG‑CGGGCGAAGCCCTCCAAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTAc                                               <  4:314299/133‑1 (MQ=255)                                                                               ttttttttGGGGCCGCTTGGGGGCTTTTGGTCGCT‑AACGC‑TGGGCCACGCCCGCCCAGCTTTTTCCAGCAAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGcc                                           <  5:61058/131‑1 (MQ=255)                                                                                 gaTTTTGGGGCGGCTTTGCGGCCTGGGGGCGCG‑ACAGG‑AGGCCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGcccc                                         <  8:174391/135‑1 (MQ=255)                                                                                   ttttGGGGCCGCTTGGCGGCCTGGGGGCGCG‑AACGG‑CGGGCGCCGCCCGCCCAGCTTGTGCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGGGGCAGCTCGAAAAAGGTACTGCCCCTc                                       <  7:130424/135‑1 (MQ=255)                                                                                            cGCTTTGCGGCCGTGTGTCGCG‑AACGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                             <  4:80591/136‑1 (MQ=255)                                                                                             gCTTTGCGGCCTTGGGGCGCG‑AACGG‑AGGGCGAAGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                             <  6:86481/135‑1 (MQ=255)                                                                                                  gCGGCCTGGTGGCGCG‑AACGG‑CGGGCGAAGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                       <  4:57243/136‑1 (MQ=255)                                                                                                   cGGCCTTGTGGCGGT‑AATGG‑AGGGCGACGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                        <  8:285207/134‑1 (MQ=255)                                                                                                   cGGCCTTGGGGCGCG‑AACGG‑CGGGCGACGCCCGCCCAGCTTTTTCCAGGCAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                        <  7:22340/134‑1 (MQ=255)                                                                                                   cGGCCGTGTGGCGCC‑AACGG‑CGGGCGACCCCCGCCCAGCTTTTTCCCGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                        <  7:186891/134‑1 (MQ=255)                                                                                                        tgggggcgcg‑AACGG‑GGGGGCAACCCCGCCCAGCTTTTTCCTGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTATTGCCCCTCGCAAGGAGGGGCAGTGTGCTg                  <  7:244940/129‑1 (MQ=255)                                                                                                          gtggCGCG‑ACAGG‑CGGGCTAAGCCCTCCCAGCTTTTTCCAGCAAAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGc                <  3:118157/135‑1 (MQ=255)                                                                                                           tgtCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGa                                                        >  7:23106/1‑94 (MQ=255)                                                                                                           tgtCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGa                                                        <  8:23106/94‑1 (MQ=255)                                                                                                            gtCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCtt              >  8:62471/1‑135 (MQ=255)                                                                                                                   aaaCG‑AGGGCCAAGCCCGCCCAGCTTTTTCCCGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGGGGCAGCTAGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca        <  7:43027/135‑1 (MQ=255)                                                                                                                    aaGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca        >  8:334487/1‑134 (MQ=255)                                                                                                                      cggcgggCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca        <  8:232610/129‑1 (MQ=255)                                                                                                                          gggCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAGCATgg  <  2:213332/135‑1 (MQ=255)                                                                                                                                     |                                                                                                                            CCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGCCGCTTTGCGGCCTTGTGTCGCG‑AAAGG‑AGGGCGAAGCCCTCCCAGCTTTTTCCAGGCAAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAGCATGG  >  KT2440/399174‑399427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑