| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 868,338 | A→C | 56.0% | intergenic (+27/+19) | loiP → / ← mneA | Metalloprotease LoiP/Putative manganese exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 868,338 | 0 | A | C | 56.0% | ‑4.7 / 6.3 | 19 | intergenic (+27/+19) | loiP/mneA | Metalloprotease LoiP/Putative manganese exporter |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base C (6/3); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
GAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCTGCAT‑GGCAG‑CCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCG > KT2440/868210‑868469 | gAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCTGGGGGTTGCTT‑GGCGG‑cccc > 7:117852/1‑135 (MQ=255) ttCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCGGCAT‑GGCAG‑cccccccc > 7:342981/1‑134 (MQ=255) gAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGGTGCGT‑GGGGG‑cccccccccgttt > 1:300017/1‑129 (MQ=255) tgaaCTCCCCCGGCGGCCGCGAGCCGCCTTGCTCCGCGGCCGGCGGCGATTCCGAAGGTGATGCCCCTGTATCAGGCTGCCGCCAAGGCGT‑AATTGCC‑TGATTCCG‑GGGGCTGCAT‑GGCAG‑CCCCCCCGGTTTTc < 8:282528/132‑1 (MQ=255) gTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCGGCGG‑GGGCG‑CCCCCCCGGgtttggc > 3:92297/1‑133 (MQ=255) tCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCTGCAT‑GGCAG‑‑CCCCCCGGTTTTCCCTTTa < 8:310214/135‑1 (MQ=255) aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGGTGCAT‑GGCCG‑CCCCCCCCGGGTTcccctgtg > 8:212146/1‑130 (MQ=255) aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCTGCAT‑GGCCG‑CCCCCCCGGGTTTGCCGTTAc > 7:297518/1‑135 (MQ=255) aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCGGGCT‑GGCCG‑CCCCCCCCGGTTTGCCGTTaa > 7:78549/1‑134 (MQ=255) tCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGGGGCCTGGGCCC‑CCCCCCCGGTTTTGCATTTACCCAAAcc > 7:233737/1‑135 (MQ=255) tCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGGGGCAT‑GGCCG‑CCCCCCCCGGTTTGCCGGTTCCCaacaca > 2:301402/1‑131 (MQ=255) tgctgcTCTGCCGGCGGCGTTTCGTACGGTGTTGCCGCTGTATCCGTCTCCCTCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCTGCAG‑GGAAC‑CCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCa < 6:304429/135‑1 (MQ=255) ccagggTGAGGCGGCTTTACCCGGCGGCC‑CCAACCCCT‑AAAAGCCTTCAATCGG‑GGGGGTGCAG‑GGCCC‑CCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGAt < 5:159761/130‑1 (MQ=255) tGAGGCCGCTGTATCCGGC‑GCCCCCAAACCCTAAAAGGCC‑TAAAGCGT‑GGGGCTGCAG‑GGAAC‑CCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGt < 5:364298/135‑1 (MQ=255) ccGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCTGCCT‑TGGAGCCCCCCCCGGGTTGCCGGTTA‑CCAATACCGCCCCTGGCCGTAATTTGCGAAAAGACGCGCCCGAATTCCCGTACg > 4:102759/1‑133 (MQ=255) cGT‑AAATGCC‑TGACTCCT‑GGGGCGGCAT‑GCCAG‑CCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGaa < 4:147175/136‑1 (MQ=255) gggCTGCCT‑GCCCC‑CCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGtt < 8:154474/135‑1 (MQ=255) cTGCAT‑GGCAGCCCCCCCCGGTTTGCCGTTTACCCAA‑CCACCCCCAGGCCGTCATTTGCGAATAGACCGCCCCCCGTGGCACGACCAAGAACGCCCTTGGCCCCAGGCGGCGCAACACCCGCAACCGCAGTTTGt > 2:254312/1‑135 (MQ=255) ggCCG‑CCCCCCCGGTTTTCCCTTTACCCAATCCCGCCACTGGCCTTCATTCCCGAATCGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCcg < 2:77983/135‑1 (MQ=255) | GAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGT‑AAATGCC‑TGAATCCT‑GGGGCTGCAT‑GGCAG‑CCCCCCCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCG > KT2440/868210‑868469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |