| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 990,702 | T→A | 18.0% | intergenic (‑54/+175) | CJ019_00935 ← / ← bfrD | hypothetical protein/putative TonB‑dependent receptor BfrD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 990,702 | 0 | T | A | 18.0% | 73.4 / 3.7 | 43 | intergenic (‑54/+175) | CJ019_00935/bfrD | hypothetical protein/putative TonB‑dependent receptor BfrD |
| Reads supporting (aligned to +/- strand): ref base T (18/17); new base A (2/6); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTAATGCCCATCAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAG > KT2440/990568‑990830 | ggTAATGCCCATCAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATa > 4:345914/1‑136 (MQ=255)ggTAATGCCCATCAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCAGTATGCCTTGAt > 8:361600/1‑135 (MQ=255) tAATGCCCATCAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATac > 5:172357/1‑135 (MQ=255) cccATCAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGCCCCCCGCGGCGT‑GCATTTTTCCTTTAAACACaa > 2:342808/1‑134 (MQ=255) aTCAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGcc > 3:43373/1‑136 (MQ=255) cAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCTGCGCCCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGccc > 8:16820/1‑135 (MQ=255) aGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCCCGCGGCGTGGCATT‑TGCCGTGATAGACCAAACccccc > 2:137031/1‑134 (MQ=255) ggCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGCCCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGc > 8:38004/1‑135 (MQ=255) ggCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGc < 1:368445/135‑1 (MQ=255) ggCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGc < 5:285480/135‑1 (MQ=255) ggCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCGTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGTGGCATT‑TGGCTTTATGCACAAAAGCCCCGc > 1:250113/1‑135 (MQ=255) gCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACg < 2:105767/135‑1 (MQ=255) cACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATAAACAAAAGCCCCGCTCACg > 6:299305/1‑134 (MQ=255) cAGGCCAGCGTTGATGCCAAAGAACCAGTGCAATTGTAACAGCGTCTTCTTCACCACGTTTCTCACATTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GAATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAAt < 4:184796/136‑1 (MQ=255) ggCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAACGCCCCGCTCACGGGAATGa < 3:345914/136‑1 (MQ=255) cAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGCCCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGa > 3:298637/1‑133 (MQ=255) cAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGa < 4:298637/133‑1 (MQ=255) cAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGCAc > 6:344249/1‑135 (MQ=255) cAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAAc > 8:229786/1‑135 (MQ=255) cAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAAc > 2:288608/1‑135 (MQ=255) cAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACg > 3:128291/1‑136 (MQ=255) ccAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGc > 6:69521/1‑110 (MQ=255) ccAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGc < 5:69521/110‑1 (MQ=255) ccAGTGCAATTGGAACAGCGTCTTCTTCACCAGGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GAATTATGCCTTGATACACAAACGCCCCGCTCACGGGAATGACCGGGGCCGGGAGGGCTGCtt < 7:257243/135‑1 (MQ=255) cAGTGCAATTGCAACAGCGTCTTCTCCCCCACGTTCGACACCTTGCGTGGCTGCCGCGCCCCGCGCGGCGT‑GCATTATGCCTTGATCCACAAACCCCCCGCTCCCGGGAATCACCGGGGCCGGGAGGGCTGCtttt < 4:210433/136‑1 (MQ=255) aaTTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTAAGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTAGtgtt > 3:93577/1‑136 (MQ=255) aaTTGCAACAGCGGCTTCTTCACCACGTTGCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GAATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCgtgt < 2:274078/135‑1 (MQ=255) gAACAGCGGCTTCTTCACCACGTTTCTCCCCTTGAGTGGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCt < 4:153817/135‑1 (MQ=255) gCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATg > 2:59861/1‑96 (MQ=255) gCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATg < 1:59861/96‑1 (MQ=255) gCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCGCACGGGAATTAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCgg > 3:316701/1‑136 (MQ=255) tcttctTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGc > 7:200719/1‑135 (MQ=255) cacGTTACTCACCTTTAGTTGCTGCCCCGCCCCGCGCGGCGT‑GCTTTTTGCCTTGAACCACAAAAGCCCCGCTCACGGCAATGACCGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGc < 4:100180/136‑1 (MQ=255) acctttATCACCGTGAGTTGCTGCCGCGACCCGCGCGGCCT‑GCAATATACCATGAATAACCAAACCCCCCCACACCGGAAAGAACCGGGCCCCGCGGGCAGGCTTTAGTTTTGTAATTTCGGGCCTCATTCGCgg > 8:322438/4‑135 (MQ=255) gttgcccACCTTTAGTGGCGGCGGCGACCCGGGCGGCGT‑GCATTATGCCTGTATACACAAAAGCCCCGCTCCCGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTaa < 3:37987/130‑1 (MQ=255) tttCTCCCCTTGAGGTGCGGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTCAAACACAAAAGCCCCGCTCACGGGAATGACCGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTaa < 5:265816/135‑1 (MQ=255) tgTTGCGGCCGCGCCCCGCGCGGCGT‑GCATTATGCCTTGATACACAAACGCCCCGCGCACGGGAATGACCGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGc < 5:282853/134‑1 (MQ=255) cgACCCGCGCGGGGT‑GCATTATGCCTTGAAACACAAAAGCCCCGCTCACGGGAATGACCGGGGCCGGGAGGGCTGCTTTTCGTGTTGGCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCa < 4:316701/136‑1 (MQ=255) cgACCCGCGCGGCGT‑GCATTATGCCTGGAAACACAAACGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCa < 4:182763/136‑1 (MQ=255) gACCCGCGCGGCGT‑GCATTATGCCTTGAAACACAAAACCCCCGCCCACGGGAATGACCGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCa < 6:259257/135‑1 (MQ=255) gACCCGCGCGGCGT‑GCATTAGGCCTTGAACCACAACCCCCCCGCCCCCGGAAAGGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCa < 1:42290/135‑1 (MQ=255) gcgT‑GAATTATCCCTTGATACACAAATGCCCCCCTCACGGCATTGACCGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTc < 3:171343/136‑1 (MQ=255) cctgcaTACACAAACGCCCCGCTCACGGGAAGTACCGGGGCCGGGAGGGCTGCTTTTCGGGTGGTCGGTGCGGCCCTC‑TTCGCGGCTAAACCCGCCCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAg < 2:11208/130‑1 (MQ=255) ccTTCATCCCCAAACCCCCCCCCCCCGGCAATCACCGGGGCCGGGAGGGCGGCTTTTCGTGTTGGCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAg < 6:172357/135‑1 (MQ=255) | GGTAATGCCCATCAAGGCCAGCACCAGGCCAGCGGTGATGCCAAAGAACCAGTGCAATTGGAACAGCGTCTTCTTCACCACGTTTCTCACCTTGAGTTGCTGCCGCGACCCGCGCGGCGT‑GCATTATGCCTTGATACACAAAAGCCCCGCTCACGGGAATGAACGGGGCCGGGAGGGCTGCTTTTCGTGTTGTCTGTGCGGGCCTC‑TTCGCGGCTAAAGCCGCTCCTGCACCGGCAACGCAGGAGCGGCTTCAGCCGCGAAAG > KT2440/990568‑990830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |