Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,202,757 A→T 23.4% V308V (GTA→GTT)  hcf136_2 → Ycf48‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,202,7570AT23.4% 45.3 / 2.2 36V308V (GTA→GTT) hcf136_2Ycf48‑like protein
Reads supporting (aligned to +/- strand):  ref base A (8/19);  new base T (5/2);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 7.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCACCTTCAG  >  KT2440/3202625‑3202888
                                                                                                                                     |                                                                                                                                   
tcGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGGCGAAGGTGTGACTGTGtt                                                                                                                                   >  6:277320/1‑135 (MQ=255)
        tGGCGGTCGGGCTGTATGGGCTTAGCGTGCAGAGCTGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑AAACGCCGAAGGTGTACCTGTATTGTTTTCACg                                                                                                                          <  3:174719/136‑1 (MQ=255)
         gggggTCGGGCTGGAGGGGCTTAGCGTGCAGAGCAGCCATGGCGGGCACAGTTTCCAGGAACCGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACg                                                                                                                          <  2:331864/132‑1 (MQ=255)
          gCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGGT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGcc                                                                                                                        >  4:53708/1‑136 (MQ=255)
           cGGTCGGGCTGGAGGGGCTTAGGGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGTTGGCCTATCACTGAGGGGGGGGTTGTT‑CAACGCGGAAGGTGTAAGTGTTTTGTTTTCACg                                                                                                                          <  8:259138/133‑1 (MQ=255)
           cGGTCGGGCGGGAGGGGCTTCGCGTGCAGAGCCGCGAGGGCGGGCACAGTTTCCCGGAAACGCAGACCGCGGAGGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGACGGTGTACCTGTATTGTTTTCACGcc                                                                                                                        <  6:175510/135‑1 (MQ=255)
                gggCGGGATGGGCTTAGCGGGAAGAGCAGCGATGGCGGGCACAGTTTCCAGTAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAggg                                                                                                                   <  2:329591/135‑1 (MQ=255)
                   ttGGAGGGGTTTCGCGTGCCGCTCATGGAGGGCGGGCACTTTTTCCAGGAAACGCAGACGGGTGATTGCATTTCACTGACGGCGGCTTTGTT‑CACCGACTATGTTTTACGTGTATTGTTTTCACTCCTAGGTGTaa                                                                                                               <  4:199465/135‑1 (MQ=255)
                          ggCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑GACAGCCGCAGGTGTACAGGTATTGTTTTCACGCCGAGGGGGAAAGcccg                                                                                                         >  2:39925/1‑134 (MQ=255)
                            cTTCGCGGGCAGAGCTGCGTTGGCGGGCACAGTTTCCCGGAAACGCAGACCGCGGAGGGCCTTTCAGTGACGGCGGCGTTGTT‑CAACGCCGATGGTGTACCTGTATTGTTTACACGCCGAGGGGTAATGCCCTAc                                                                                                       <  1:46785/135‑1 (MQ=255)
                               aGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑GAACGCCGCAGGTGTACCTGTATTGTTTTCACGCCGCGGGGTAATGCCCTACCAgg                                                                                                   >  3:352461/1‑136 (MQ=255)
                                 cgggCAGAGCAGCGATGGCGGGCACAGTTGCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTGGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGa                                                                                                  <  5:359165/132‑1 (MQ=255)
                                   tGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGGT‑CAACTCCGCACGTGTACCGGTTTTGTTTTTACGCCGCGGGGGTAAGCCCGACCCGgtgt                                                                                                >  8:8657/1‑135 (MQ=255)
                                        agcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGTT‑GAACGCCGAAGGTGTACCGGTTTTGTTTTCACGCCGAGGGGTTATGCCCTACCAGGAGTGAAggg                                                                                          >  3:353906/1‑136 (MQ=255)
                                        agcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGTT‑CAACGCCGAAGGTGTAGCGGTATTGTTTTTACGCCGAGGGGTAATGCCCATCCATGAGTGAAgg                                                                                           >  6:165675/1‑135 (MQ=255)
                                        agcagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGGT‑GCACGCCGCAGGTGTGCCGGTGTTGTTTTGACGCCCCGGGGGTATGGCCGACCCCGAGTGGAggg                                                                                          >  4:184703/1‑136 (MQ=255)
                                         gcagcGATGGCGGGCACAGTTTCCGGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGTTACAAC‑CCCAACGTGTAACAGTTTTTTTTTTACGCCGCGGGGTAATGGCCTACCAGGAGTGAAtgg                                                                                          >  7:114560/1‑135 (MQ=255)
                                          cagcGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGGTGTT‑GAACGCCGAAGGTGTACCGGTTTTGTTTTGACGCCGAGGGGTTATGCCCGACCACGACTGAAgggg                                                                                         >  8:72109/1‑134 (MQ=255)
                                              gttGGCGGGCACAGTTTCCAGGAAACGCAGACCGCAGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCGGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGcc                                                                                    <  4:324338/134‑1 (MQ=255)
                                              gATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGAAGGTGTACCTGTTTTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGc                                                                                     >  4:254045/1‑135 (MQ=255)
                                                     ggCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑GAAAGACCAAACTGTTAGCGTATTTTTTTTAAGGCGACGGGTGATTACCAACAACGAGTCAAGAGTTGGCCAcaac                                                                               >  8:5344/1‑131 (MQ=255)
                                                      caacaGTTTCCAGGAAACGCAGACCGCGGAGGGCCTTTCACTGACGGCGGCGTTTTT‑CAACGCCTAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCt                                                                             <  5:165675/133‑1 (MQ=255)
                                                          aGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGGTGGCCCCTCGCTCAGaa                                                                        >  4:297578/1‑136 (MQ=255)
                                                                cAGCAAACGCAGACCGCGGATGGCCTTTCACTGCCGGCGGCGTTGTT‑CGACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCa                                                                    <  6:151833/134‑1 (MQ=255)
                                                                    aaaGGCAGCCGGCGGTTGGCCTTTCAGTGACGGCGGGGTTGTT‑AAACGCCGAAGGTGTACCTGTATTTTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCAt                                                               <  1:64529/135‑1 (MQ=255)
                                                                                cgGTTGGCCTTTCACTGACGCCGGCGTTGTT‑CAACGCTTAAGGTGTACGTGTATTTTTTTCACGCCGATGGGTAATGCCCTACCAGGAGTGAAGTGTTGGCCACTCTCTAAGAAGCCAGGCATCGCAAACCGATg                                                   <  6:197484/135‑1 (MQ=255)
                                                                                   ttGGCATTTCACTGACGGCGGCGTTGTT‑AAACGCCGATGGTGTACCTGTATTTTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcct                                                <  7:5344/134‑1 (MQ=255)
                                                                                   aTGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcct                                                <  7:48131/135‑1 (MQ=255)
                                                                                     ggCCTTGCACTCACGGGGGCGTTGTC‑CACCGCCGACGGGGGACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATgcctgg                                              <  3:5200/135‑1 (MQ=255)
                                                                                              cTGACGGCGGCGTTCTT‑CAAGGCCGAAGGGGTACCTGTTTTGTTTTGACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGGGGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCt                                    <  3:164868/136‑1 (MQ=255)
                                                                                                gcgggcggcGTTGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGCCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTg                                  <  3:311391/133‑1 (MQ=255)
                                                                                                                      cGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCg              <  6:342276/135‑1 (MQ=255)
                                                                                                                      cGAAGGGGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGt             <  3:53708/136‑1 (MQ=255)
                                                                                                                       gAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGt             <  6:50240/135‑1 (MQ=255)
                                                                                                                           gtgtACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCAGCGCAAACCCAATCCTGGCCGGTTTTATTTGCGGGGGGGATTGGGGGTAGTggga         >  6:248244/1‑133 (MQ=255)
                                                                                                                                 cTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCCTCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCACCTTCAg  >  3:248277/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TCGTGTGCTGGCGGTCGGGCTGGATGGGCTTAGCGTGCAGAGCAGCGATGGCGGGCACAGTTTCCAGGAAACGCAGACCGCGGATGGCCTTTCACTGACGGCGGCGTTGTT‑CAACGCCGAAGGTGTACCTGTATTGTTTTCACGCCGAGGGGTAATGCCCTACCAGGAGTGAAGGGTTGGCCACTCGCTCAGAAGCCAGGCATCGCAAACCGATGCCTGGCCTGTTTTCTTGCCTGGGCGAATGGGTGGTCGTGGCACCTTCAG  >  KT2440/3202625‑3202888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑