| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,491,323 | T→G | 47.1% | intergenic (‑685/+36) | CJ019_03306 ← / ← CJ019_03307 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,491,323 | 0 | T | G | 47.1% | ‑0.6 / 3.6 | 32 | intergenic (‑685/+36) | CJ019_03306/CJ019_03307 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (6/7); new base G (3/9); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCA > KT2440/3491194‑3491448 | gCCCGCTCCTACATCGACCGGGGGTGGCGCAACGTTGTCGGCGCGGGTTAACCCGCTAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATCCACCGCCCCCGCCGCCACCGCATTCGCGGGCAGCCGGCCcgc < 4:114602/136‑1 (MQ=18) tCGCCCGGGGGTGTCGCCACGTTGTAGGAGCGGGTTTACCCGCTAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATCCACCGCCACCCCCCCCCCCGCATCCGCGGGCAGCCGCCCCGCGA‑ACACCGgcgc < 2:33647/135‑1 (MQ=21) ccGGGGGCGGCGCCACGGGGGCGGCGCGGGTTACCCCGCGAATAGGCCGGACCGGCCGGCCACTCTCCCGGGCCAACCCCCGCCCCCCCCCCCCCCCCATCCCCGGGCCGCCGCCCCGCCA‑ACACCGGCGCAGCCg < 4:352091/136‑1 (MQ=255) cGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCGCGC‑ACACCCGCGCAGCCgg > 4:131180/1‑136 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCCCGC‑ACACCGCCGCCGCCgg > 5:161793/1‑135 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCTGCCCCCGC‑ACACCCGCGCAGCCggg > 4:207967/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGA‑ACACCGGCGCAGCCgg > 8:289971/1‑135 (MQ=255) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTACCCGGCACCCCGCCCGCGC‑ACACCCGCGCCCCCgg > 2:274261/1‑135 (MQ=21) ggtggtGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCAGGCCACTATCACTGGTCATTCACCGTCACAGCCGCCACCGCATTCCCCCGCAGCCACCCCGCCC‑ACACACGCGCAccccgg > 4:5929/1‑133 (MQ=18) cccccAGGTTTTAGGCGCTGGTTAACCGGCAAATAGCCCGGACCTCCCGCCCAAAACCCGTGGACATTCCCCGCCACCCCCCCCCCCGCATGCGCGGGCAGCCGCCCCGCCA‑ACACCGGCGCAGCCGGTTTCATc < 6:333738/132‑1 (MQ=255) ggTTGTCGGAGCTGGTTTCCCCGCTAATTGGCGGGACCGGCCGGCCACTATCCCTGGTAATTCACCGTCACAGCCGCCCCCGCATGCGCGGGCAGCCGGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTc < 7:217806/134‑1 (MQ=255) ggTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCGGCCCGCGC‑ACACCGGCGCCGCCGGGTTGGTCCCACTCACAGAcccacggc > 7:346293/1‑129 (MQ=18) ttACCCGCTAATAGGCCGGACCGGCCGGCAACTATCCCGGGTCATACACCGCCACCGCCGCCACCCCATGCGCGGGCAGCCTCCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTg < 3:309462/135‑1 (MQ=255) gcgAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCAGCCCGCGA‑ACACCGGCGCCGCCGGGTTACTACAACTACACGCCCCTCGACCGGTACGc > 8:122721/1‑134 (MQ=18) gcgAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCACCCAGCCCGCGA‑ACACCCGCGCCCCCGGGTTCATCCAACACCACCACCCACGACCCGTACGCa > 6:202428/1‑135 (MQ=18) gcgAACAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGA‑ACACCCGCGCCGCCCGGTTCCTCCAACTCCACCCCCCTCCACCGGTACGc > 2:235709/1‑134 (MQ=18) tAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCCGCCGGCCCGCGC‑ACCCCCGCGCACCCGGGGTCCTCCCACTCAACGAACAAGGCCCGGTGCGCAAAccgg > 4:308096/1‑133 (MQ=18) gCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGtct < 7:289971/134‑1 (MQ=255) aCTATCCCTGGGCATTCCCCGGCCCAGCCCCCCCCGCATGCGCGGGCAGCCGGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCtt < 8:366279/135‑1 (MQ=255) aTTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGA‑ACACCCGCGCAGCCCGTTTCATCCAACACAACCACCATCGACCTGTACCCAAACGTCTCTTCCATCCATTGCCTTGAATGGCCAATTGt > 4:230879/1‑136 (MQ=255) ttCACCGGCACCGCCTCCACCGCATTCGGGGGAAGCGTGCCCGCGA‑ACACCGGCTCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGt < 8:12993/135‑1 (MQ=255) ccGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTg > 3:363011/1‑136 (MQ=255) gcacccccgccCCGGAATTCGCGGGCAGCCCGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGAt < 6:91153/129‑1 (MQ=255) cgccgccCCCCCATGCGCGGGCAGCCTGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAAt < 3:153366/135‑1 (MQ=255) gccgccCCCCCTTTCGCGGGAAGCCTGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAAt < 6:103276/135‑1 (MQ=255) gcccccACCGAAGGCGCGGCCAGCGCCCCCGCCA‑ACACCGGCGCAGCCGGTTTCATCCAACTCACCGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAAt < 2:128240/135‑1 (MQ=255) ccgccACCGCATGCGGGGGAAGCCTGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATg < 1:245718/135‑1 (MQ=255) cccccccGCATTCGGGGGCCCCCCCCCCCCCACACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATg < 7:171141/130‑1 (MQ=255) ttCGCGGGAAGCCGCCCGGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGaa < 3:76890/136‑1 (MQ=255) gcgggggCAGCGTGCCCGGCA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGaa < 6:69535/131‑1 (MQ=255) ccgcgGGCAGCCGGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGaa < 3:131180/134‑1 (MQ=255) gcgGGCAGCCGCCCCGCGA‑ACAGCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCGATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGca < 3:230879/136‑1 (MQ=255) | GCCCGCTCCTACATCGACCGGTGGTGTCGCCACGTTGTAGGAGCTGGTTTACCCGCGAATAGGCCGGACCTGCCGGCCACTATCCCTGGTCATTCACCGTCACAGCCGCCACCGCATTCGCGGGCAGCCTGCCCGCGA‑ACACCGGCGCAGCCGGTTTCATCCAACTCAACGACCATCGACCTGTACGCAAACGTCTCTGCCATCGATTGCCTTGCATGGCCAATTGTCGTTGATGTAATGATCCGGCTTGAAGCA > KT2440/3491194‑3491448 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |