| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,893,888 | A→C | 20.9% | intergenic (+26/‑472) | lrpC → / → pucI_2 | HTH‑type transcriptional regulator LrpC/putative allantoin permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,893,888 | 0 | A | C | 20.9% | 36.5 / 2.1 | 34 | intergenic (+26/‑472) | lrpC/pucI_2 | HTH‑type transcriptional regulator LrpC/putative allantoin permease |
| Reads supporting (aligned to +/- strand): ref base A (7/19); new base C (5/3); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
GATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCT > KT2440/4893754‑4894019 | caTGGCGCAGCTGGACCCCCTCCTCGACCTCATGAATGGCCTGGCCGCCACCAATCCCGCCAGCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAg < 3:228858/135‑1 (MQ=255) gAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGt > 4:54150/1‑136 (MQ=255) cTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCCGGCGGGGCc‑gc > 4:25211/1‑136 (MQ=255) tGGACACCCTGCTCGACCTCATGACTGTGCTGGCCGAGACCAATACCGCGATCATCAAGACGCCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCc‑gc < 6:304489/135‑1 (MQ=255) cacCCTCCCCCACCCCATGAATGTGCTGGCCGAGCCCAATCCCGCGATCATCAAGACCACCCCGGGCAAGCGGCGGTTGCCGCCAATGGATTGAGTGGCTCCTGGGCTGGCC‑TCTTCCCCGGCGTGCCC‑GCGAag‑a < 4:104835/136‑1 (MQ=255) tCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑GAGTCGCACGGGTGGCC‑CCGAAC‑AGGG‑CAG‑t > 2:200564/1‑135 (MQ=255) gagaCCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCATCGCAGGCGTGCCC‑GCCAAC‑AGGC‑CAG‑TACACGAAAATCTGAGTAtttttt > 8:362998/1‑135 (MQ=255) cccAATACCGCGATCATCAAGACGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTGCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTa < 6:133841/134‑1 (MQ=255) aaTACCGCGATCATCACGACGACCCCGGCCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCTTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTacac < 1:207502/135‑1 (MQ=255) tACCGCTATCAGCAAGACTCCCCCGGTCAAGCGGCGGTTGCCGCAAATGGATTTATTGGCTCCTGGGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACaaa < 4:56816/136‑1 (MQ=255) tcatcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGGCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGCGTATTTTTTTTACAAAAAAACG‑TAAc > 4:44359/1‑136 (MQ=255) tcatcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TAATCGCACGCGTGGCC‑GCGCAC‑ACGG‑CAGAGACA‑GAAAAGCAGATGATTTTTTTTTCACaaaaaactta > 6:84547/1‑130 (MQ=255) tcatcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TAATAGCAGGCGTGCCC‑GCGAAG‑AGGG‑CAC‑GACAGGCAAAGCTGAGTATTTTTTTTACAAAAAAAccgaa > 7:257854/1‑131 (MQ=255) atcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTAGCAGGCGTGGCC‑GCGGAG‑AGGG‑CAG‑GTCAGGTAAAGCTGTGTTTTTTTTTTTAACAAAAACG‑TAAc > 5:153055/1‑134 (MQ=255) tcaAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCAt < 4:274213/136‑1 (MQ=255) aagaagACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCGTGGCCGGGGTGCCC‑CCGCAG‑ACGCGCCC‑TA‑AGGAAAAACAGGGGTTTTTTTTTAAACACAAACC‑TAAcacaa > 8:203256/1‑131 (MQ=255) gaCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTTGCTCCTGTGCTGGCCAAATTCCCCGGCGGCCCC‑GCCATG‑AGCC‑CAG‑AAAAGAAAAACATATTAATTTTTTTTAAAAAAAAACG‑AAACCAAAgttc > 2:293913/1‑132 (MQ=255) aCCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGcc < 6:58697/135‑1 (MQ=255) gTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCGGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTtg < 4:128467/136‑1 (MQ=255) aaGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑ACATGGGCGGCGTGCGC‑CCGGAGTCGGC‑CGG‑T‑CAAGAAAAACCGCGGATTTTTTTTTAACCAAAACGTTAACAAACGGGGCCTTTTTtgg > 6:302300/1‑134 (MQ=255) aGCGGCTGTTGCCGCCAATGTATTTATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTtgtgt < 6:140800/135‑1 (MQ=255) gcggcgGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGc < 3:54150/136‑1 (MQ=255) aTGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGgcg < 4:22898/136‑1 (MQ=255) aTGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGgc < 6:299805/135‑1 (MQ=255) attgattgGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGcccc < 3:25211/136‑1 (MQ=255) ttgattgGCTCCTGTGCTGGCC‑TCTGGCCAGGGGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGcccc < 7:322881/135‑1 (MQ=255) ggattgGCTCCTGTGCTGGCC‑TCTTCGCAGGCGGGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGcccc < 2:229276/133‑1 (MQ=255) gccccTGTGGGGGCC‑GCTGCGCCGGCGGGCCC‑GCGAAG‑CGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGtt < 3:207117/133‑1 (MQ=255) gCTCCTCTGCCGGCC‑TCTTCGCCGGC‑TGCCCTGCTTTT‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGt < 5:33917/135‑1 (MQ=255) cTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGtt < 6:168867/135‑1 (MQ=255) cTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGtt < 7:362998/135‑1 (MQ=255) cTGTGCTGGCC‑ACATCGGCGGCGGGGCC‑GCGGAG‑AGGC‑CAG‑GAACGGGAATGGGGTGGTTTTTTTGTACACAAAAACG‑TAAACAGAGGTGCCCTTTTTTTGTGCACGTTGTTGAGTGGAGGCGCAAATCggtta > 6:182492/1‑130 (MQ=255) gCTGGCC‑GGTTCGCAGGCGGGCCC‑GCTAAT‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACt < 5:148107/135‑1 (MQ=255) cGCAGGCGTGCCG‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCctct < 3:356597/135‑1 (MQ=255) | GATGGAGCAGCTGGACACCCTGCTCGACCTCATGAATGTGCTGGCCGAGACCAATACCGCGATCATCAAGAAGACCCCGGTCAAGCGGCGGTTGCCGCCAATGGATTGATTGGCTCCTGTGCTGGCC‑TCTTCGCAGGCGTGCCC‑GCGAAG‑AGGC‑CAG‑TACAGGAAAAGCTGAGTATTTTTTGTACACAAAAACG‑TAACCATAGGTGCCATTTTTGTGTGCACTTTCTTCAGTGGCGCCCCAATTCGTTACACACTTTCCACCCTCT > KT2440/4893754‑4894019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |