| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 382,484 | T→G | 39.9% | S14A (TCC→GCC) | soxD → | Sarcosine oxidase subunit delta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 382,484 | 0 | T | G | 39.9% | 18.8 / 5.0 | 40 | S14A (TCC→GCC) | soxD | Sarcosine oxidase subunit delta |
| Reads supporting (aligned to +/- strand): ref base T (8/14); new base G (8/8); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTATTCACCATGAA > KT2440/382354‑382612 | ccGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGCTCGACGAACCCGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGCCACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTGCGCGcccg > 2:102797/1‑133 (MQ=255) gTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCCCTGCGGCGCGCGGCGCGCCGGAGAAGAGTTCCACGCCGCTGGc > 3:320235/1‑136 (MQ=255) gTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGc > 4:258097/1‑136 (MQ=255) caaCAACGGCGCCCTGATCGACGAACCCGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGGCCCCACTGCGGCGAGCTGCGCGCCGAAGAAGAGTTCCACGCCTCTGGCCa < 8:269290/132‑1 (MQ=255) tACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGccccgc > 4:228597/1‑132 (MQ=255) aCTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCCCTGCGCGCCGAAGAAGACTTACACGCCACAGGCCAGGCGCACATTgcc > 6:181519/1‑135 (MQ=255) aCTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTgcc > 8:272799/1‑135 (MQ=255) cgcccaaCCCGGCGCCGCCGCCGGCGCCCAATAACCG‑GAGACACCGTCTTGTTGCATATTTTCTGCCCCCCCTCCGGCGAGCGGCGCGCCGAAGACGAGGTCCACGCCTCTGGCCAGGCGCACATTGcccgcccg < 7:17811/130‑1 (MQ=255) cgacgaACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCGCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGcccgcccg < 2:244083/135‑1 (MQ=255) gacgaACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCccgcccgc > 8:223682/1‑135 (MQ=255) gacgaACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCCCAGAAGAGATCCACCCCCAATGCCACGCGCACAATgcccgccccc > 1:284597/1‑133 (MQ=255) gacgaACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCGCCCCAGAAGAGTTACACGCCTCTGGCCCCGCGCACCATGCCCGCCCCCt > 4:23839/1‑136 (MQ=255) cgccgccgTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGGGCGGGGCGGCGCAGAAGAGTTGCCCCCCGCTGGCCCCGCGCCCAGTGCCCCCCCCCGGGGCCCGAACGcc > 1:314997/1‑135 (MQ=255) gccgccgGCGCCAAC‑AACCGCGCCACCCCGTCTTTTTTCATATTTTCTGTCCCCACTGCGGCGAGCGGCGCTCCGAAGAACAGTCCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCt < 1:349153/135‑1 (MQ=255) cgTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTGCGCTCCGAACAAGAGTTACACGCCTCTGGCCAGGCGCACATTTCCCGCCCGCTGGCCCCTAAAGCCTGCTCcg > 4:158805/1‑136 (MQ=255) gcGCCCACTCCCCG‑GCCACACCGTCTTGTAGCATATTTTCTGCCCCCACGGCGGCGCGCTGCGCGCCGACGAAGCGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCcgac < 3:310920/135‑1 (MQ=255) cACTAACCG‑CAGACACCGTCATGTTGCATATTTTCTCCCCCCACGGCGGCGAGCTGCGCTCCTAAGAAGAGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACgagga < 3:23839/136‑1 (MQ=255) caaaCCG‑GACACACCGTCATGTTGCATTTTTTCTGACCCCACTGCGGCGAGCGGCGCGCCGAAGAAGAGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGt < 3:258097/134‑1 (MQ=255) gaaCCG‑GAGACACCGTCATGTTGCATATTTTCTGGCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGt < 8:22944/134‑1 (MQ=255) cgttcccacCGTCATGTTGCATATTTTCTGCCCCCCCTGCGGCGCGCGGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTgggg < 5:181519/129‑1 (MQ=255) gagaCACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGCGCTGCGCGCCGAAGAAGAGTTCCACGCCGATGGCCAGGCGCACATTTCCCCCCCCCTGGCACCTCACGCCTGCTGCGACGAGGAGGGGGGTaa > 4:130445/1‑135 (MQ=255) ccgaCACCGTCAGGTTGCATATTTTCTGTCCCCACTGCGGCGAGGTGCGCTCCTAAGAAGAGTGCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTa < 8:279017/133‑1 (MQ=255) gaCCCCGTCAGGTGGCATATTTGCTGGCCCCACTGCGGCGAGCTGCGCCCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTAcc < 6:299670/135‑1 (MQ=255) gaCACCTTCATTTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCGCCGAAGACGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTAcc < 7:3065/135‑1 (MQ=255) gaCACCGTCATGTTGAATATTTTCTGTCCCCACTGCGGCGAGCTGCGCGCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTAcc < 5:152695/135‑1 (MQ=255) gaCACCGTCATGTTCCATATTTTCTGTCCCCCCTCCGGCGCGCTGCGCTCCGAAGAAGTGGCCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTAcc < 5:152701/135‑1 (MQ=255) cATGTTGAATATTTTCTGCCCCCCCGGCGGCGCGCGGCGCTCCGAAGAAGAGGCCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGtt < 2:314997/135‑1 (MQ=255) aTGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCCGGCGCACATTGCCCGCCCGCGGGACCCTAACGCCTGCTCCGCCGAGGAGTGGGGTACCTACATGTTc > 1:318047/1‑135 (MQ=255) aTGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTc > 4:35213/1‑135 (MQ=255) tGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTAc < 7:223682/134‑1 (MQ=255) atTTTCTGTCCCCACTGCGGCGAGCTGCGCGCCGAAGAAGAGTTACACGCCTCTGGCCCGGCGCACATTTCCCGCCCGCGGGACCCGAACGCCTGCGCCGCCGAGGAGTGGGGTAACTACATGTTCTACCCTGa > 8:9418/1‑134 (MQ=255) tCTGGCCCCACTGCGCCGAGCTGCGCGCCGAAGAAGACTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGT‑GGGTACCTACATGTTCTACCGTGACAAccc < 3:111130/135‑1 (MQ=255) cTGTCCCCACTGCGGCGCGCTGCGCGCCCAAGAAGAGTTCCACGCCTCTGGCCACGCGCACATTGCCCGCCCGCGGGCCCCGAACGCCTGCGCCGACGAGGAGTGGGGGACCGACAAGTTCTACCGGGACCAccc > 7:81813/1‑135 (MQ=255) cTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAAccc < 4:138016/135‑1 (MQ=255) cTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAAccc < 3:35213/135‑1 (MQ=255) tGTCCCCTTTCCGGCGAGCGGCGCTCTGAATAAGAGTGCCTCGACTCTGGGCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAAccc < 1:336629/134‑1 (MQ=255) cccACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCgg < 4:145591/136‑1 (MQ=255) cACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTa < 4:127618/136‑1 (MQ=255) cggcgAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTATTc < 4:42435/134‑1 (MQ=255) cggcgcACCGATGCTGAGGTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTATTCACCATGaa < 6:129876/133‑1 (MQ=255) | CCGCGCCGTTCTCCATGGACCGTTTCTACAACGGCGCACTGATCGACGAACACGGCGCCGCCGCCGTCGCCCACTAACCG‑GAGACACCGTCATGTTGCATATTTTCTGTCCCCACTGCGGCGAGCTGCGCTCCGAAGAAGAGTTCCACGCCTCTGGCCAGGCGCACATTGCCCGCCCGCTGGACCCTAACGCCTGCTCCGACGAGGAGTGGGGTACCTACATGTTCTACCGTGACAACCCGCGCGGTATTCACCATGAA > KT2440/382354‑382612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |