| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,780,706 | 0 | A | T | 57.1% | ‑1.5 / 10.2 | 21 | intergenic (+96/‑29) | ahpC_2/ahpF | Alkyl hydroperoxide reductase C/Alkyl hydroperoxide reductase subunit F |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base T (11/1); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGG > KT2440/2780587‑2780823 | cTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACCCCCGGGCGACCTCGCC‑AGGGC‑GGTTTTTTTTGCGAGTTTTGaaa > 3:362310/1‑134 (MQ=255) taCCTCTTAGGAAATATCTAAGGTCATGCCAATAACGCGGGCGTTAAGCAGCCGCAAAAGCTTAAGTACCTACCGCCCCTTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTTTATCCGAGTTTTTAAAAAgg < 1:14684/134‑1 (MQ=255) tAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACCCCCGGGGGGCCACGGC‑GGGGC‑GGGTTTGTATGCGCGTTTTGTAAAAGGCATCGCCCGGCTGTTggg > 7:202003/1‑134 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GGTTTTGTTTCCGAGTTTTGAAAAAGGAATCGCCCGGATGTTGGCCGCCACGCt > 6:190102/1‑134 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑GGGGC‑GTGTTTGTTTCCGCGGTTTGGAAAAGGAATCGCCCGGTTGTTGGGCGCCCCGcg > 7:104903/1‑133 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑GGGGC‑GGTTTTGTTTCCGAGTTTTGAAAAATGCAAAGCCCGGTTGTTTGGCGCCACgag > 6:252768/1‑132 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCCGGCGCCCGCGCC‑TGGGC‑GTTTTTGTTTACCAGGTTTTTAAAAAGAATCGCCCGGATTTTGGACGCCACGcg > 8:14803/1‑133 (MQ=255) cccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACCCCCGGGCGACCTCGCC‑TGGGC‑GGTGTTGTAGCCGAGGTTTGAAAAAGGATTCGGCCGGATTGTGGACGCCCCGcct > 3:338759/1‑133 (MQ=255) cccATAACGCGGGCGGTAAGCAGCAGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCCACCTAGCC‑TGGGG‑GTTTTTGTATACGAGTTTTTAAAAATGCATGGCCCCTAAGTTGGGCGCCAccgg > 5:242577/1‑131 (MQ=255) ccATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAAACCCCGGGGGCCCACGCC‑AGGGCGGGTTTTGTTACGGGGTTTTG‑AAAAGGAATCGCCCCGATGTTGGTCGcccccggg > 2:253579/1‑128 (MQ=255) cgcgAGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGGTTTGAAAAAGGGATCGCCCGTATGTTGGACGCCACGCTTAAATCg > 4:172708/1‑134 (MQ=255) cggTACGCCGCCGCCAAAGCTGAAGTACCAACCGCCCCGTAAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGa < 1:112699/135‑1 (MQ=255) cggTAAGCAGCCGCCAATCGTGAAGGACCTACCGCCCGGTCAAACGCCCGGGCGACCGGGGC‑TGGGC‑TTTTTTGTATCCTAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACt < 6:237761/133‑1 (MQ=255) ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGCCCTCGCC‑TGGGC‑GTTTTTGTTTGCGAGTTTTGAAAAAGGAATCGGCCGGTTGGTGGCCGCCCCGCGTAAATCGCAACTGa > 3:354147/1‑134 (MQ=255) ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGCCCTCGCC‑GGGGC‑GGTTTTGTTTGCGCCGTTTGAAAAATGAATAGCCCGGATGTTGGTCGCCCCGCCTAAAACGCAAcagt > 7:289758/1‑131 (MQ=255) ccAAAGCTGATGTACCTCCCGCCCCGGCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGa < 4:240329/135‑1 (MQ=255) cGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGATTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGAt < 7:188279/134‑1 (MQ=255) cccGTCAAACGCCCGGGCGCCCTCGCCGGGGGC‑GTTTTT‑TTTAAGAGTTTTGAAAAAGGAATAGCCCGGATGTTGGCCGCCACGCGTAAAAAGGAACAGAAAACCAACATGGAGCGGGTCACCCACCCgcacaa > 8:76930/1‑129 (MQ=255) gTCAAACCCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTTTCCGCGTTTTGTAAAAGGAATAGCCCCGATGTTGGGCGCCCCGCGGTAATAGCCACTGAAAACCAACATTGTGCGGGGGACCCCCCCGATCGCGAta > 6:64949/1‑134 (MQ=255) cgcccTCGCC‑GGGGC‑GTTTTTGTTGCCGAGGTTGGCAAAAGGAATCGCCCGGATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTcga < 5:5013/132‑1 (MQ=255) cGCC‑TGGGC‑GTTTTTGTTTCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCCCGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGCTCGAGATCGTTGCGTCCCTCGACGACgg > 8:194556/1‑135 (MQ=255) | CTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCC‑TGGGC‑GTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGG > KT2440/2780587‑2780823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |