| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,285,747 | 0 | T | G | 52.2% | 1.5 / 11.0 | 23 | G658G (GGT→GGG) | tamB | Translocation and assembly module subunit TamB |
| Reads supporting (aligned to +/- strand): ref base T (5/6); new base G (4/8); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAGTGGCA‑GTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGGCGACGACCAGCGACTGGCCCCCGA > KT2440/3285626‑3285878 | gCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAGTgg < 5:97610/134‑1 (MQ=255) ggacTTGACCGTGGCGCTGGACGGCCCGCATCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTTGCCGAATCCAGGCCGGTGGCCAGGAGTGGCA‑GTTGCa < 8:277771/132‑1 (MQ=255) gACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGCCACGGGCCGCAACCCGGCCGGGGGGCCCGCGTGGGC‑GTTTGAGGcgcc > 3:174712/1‑135 (MQ=255) cgcTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGCCCCAGGCCGCATACCCGCCCGGGGGCACGCGTGGGC‑GTTTCAGGcgccggcccgcc > 8:169721/1‑135 (MQ=255) gcTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGCCCCAGGCCGCATCCCAGCCCGGGGCCCGGGTTGGCC‑GTTTCATGcccccgccccccg > 2:281370/1‑129 (MQ=255) gcTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACGGGCCACGGCGCGCATCCAGGCCGGTGGGCAGGAGTGGCA‑GTTGCACGcgcctgcccgcca > 4:232195/1‑134 (MQ=255) cGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACAGGCCGCAACCCCGCCCGTGGGCCCGCGGGGCC‑GTTTCAGGcgccagcccgccagcaccc > 6:360794/1‑132 (MQ=255) cccTTGGCCGGGAGGGCCAGTTCAACAAAGGGGCCGGGCGCGGCCGACGGGCCACTGGCCGAAGCCCGGCCGGGGGCCAGGAGTGGCA‑GTTGCCGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCt < 4:173089/132‑1 (MQ=255) tgggCCGGGATGCCCAGTTCAACAACGGGCCCGGGCGCGGCCGACTGCCCCCTGGCCGAAGCCCGGCCGGGGGCCAGGAGGGGCA‑GTTGCAGGCGCCGGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACt < 3:147511/133‑1 (MQ=255) gggATGGCCTGTTGAACAACGGTGACTGGCGCGGCCGACTGGCCACTGGCCCAATCCCGGCGGGGGGCCAGGAGTGGCA‑GTTGCCGGCGCCGGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCgggg < 1:11756/134‑1 (MQ=255) gTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATACAGGCCGGTGGCCAGGAGTGGCA‑GTTGCAGGCGCCTGCACGCCAGCCGCGGCTGGCCAGCGGCCCGCTCGCCTTAGGGGCCCActgctg > 4:265049/1‑135 (MQ=255) cAACGGGGACGGGCGCGGCCGACTGGCCGCTGGCCGAATCCAGGCCGGTGGCCAGGAGGGGCA‑GTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTc < 8:96831/135‑1 (MQ=255) aGGTGACTGGCGCGGCCGAGGGGCCCCAGGCCCAAACAAGGCCCGGGGGCAGGCGTGGCA‑GTTTCAGGCGCCGGCCCGCCGGCCGCGGCGGGCCCCCCGCCCGCGCGCCATCGGGGGCCCCCGCGTGCGCTcccg > 6:334976/1‑133 (MQ=255) gTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAGTGGCA‑GTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGcc < 8:228252/135‑1 (MQ=255) aggggCGCGGCCGACTGCCCACGGGCCGAATCCAGGCCGGGGGCCAGGAGGGGCA‑GTGGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAgg < 3:128374/132‑1 (MQ=255) tGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAGTGGCA‑GGTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCa < 7:290647/135‑1 (MQ=255) tGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGGGGCCAGGAGTGGCA‑GTTGCAGGCGCCGGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCa < 2:209882/135‑1 (MQ=255) gggCGCGGCCGACTGGCCACGGGCCGAATCCAGGCCGGGGGCCAGGAGTGGCA‑GTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCa < 7:284147/134‑1 (MQ=255) cctactCCCGGCCGGGGGACAGGAG‑GGCATGTTGCAGGCCCGGGCACGCCGGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGGGTGGCGAGGACCAg < 6:274332/128‑1 (MQ=255) ccGAATCCCGGCCGGGGGCCAGGAGTGGCA‑GTGGCCGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGGCGACGACCAGCg < 7:65712/135‑1 (MQ=255) gAATCCCGGCCGGTGGCCAGGAGGGGCA‑GTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGGCGACGACCAGCGAc < 8:179396/135‑1 (MQ=255) cAGGCCGGTGGCCAGGAGTGGCA‑GTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGGCGACGACCAGCGACTGGcc > 2:250551/1‑135 (MQ=255) cGGTGGCCAGGAGTGGCA‑GTTGCAGGCGCCTGCCCGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGGCGACGACCAGCGACTGGCCCCCGa > 8:65463/1‑135 (MQ=255) | GCCAACAGGCCTTGACCCTGGCGCTGGACGGCCCGCAGCTCAAGCTCGACCTTGGCCTGGATGGCCAGTTGAACAAAGGTGACTGGCGCGGCCGACTGGCCACTGGCCGAATCCAGGCCGGTGGCCAGGAGTGGCA‑GTTGCAGGCGCCTGCACGCCTGCAGCGGCTGGCCAGCGGCCAGCTCGACTTCGGGGCCCACTGCTGGCGCTCCGGCCAGGCAAGCCTGTGTGGCGACGACCAGCGACTGGCCCCCGA > KT2440/3285626‑3285878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |