| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,348 | 0 | T | G | 46.2% | ‑6.6 / 10.5 | 27 | A19A (GCT→GCG) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (13/1); new base G (9/3); total (23/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTG > KT2440/3440226‑3440478 | cAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCAGGCGGCGCCCgcgc > 3:6445/1‑137 (MQ=255) tAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCGGGgt > 6:156706/1‑137 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCAGCCTGGgtgt > 6:99231/1‑137 (MQ=255) gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCAGCCTGGgggt > 4:265921/1‑137 (MQ=255) gcggtcggaTCCCACGGCCCTCAACACGCGCGCGCTCGTGGTGCGGCTGTGAGCGCTGGGCCCC‑CCCGCCTGGTCGCGGGGGCGCGGCTGCGGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGc < 8:205256/131‑1 (MQ=255) cTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGcc > 2:327585/1‑137 (MQ=255) aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCTGGCGGCGCCCCGGCACCCTGGGTGTGGCAGGCCAAcc > 7:259046/1‑136 (MQ=255) tgcGCACGGCCCTCAACACGCGCGCGCGGGTGGGGCGGCGTTGCGCGGTGGGGCCC‑CCCGGGGGGGCGCGGGGGGGGTGGGGGGGGCCGCCCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCt < 6:165227/135‑1 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCCACGCCGATaaa > 1:210789/1‑136 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCAGGGTGTGGCAGGCCAACGCCGATaaa > 8:168531/1‑136 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCACGCCTATAAg > 5:68789/1‑137 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGCCGGCCCCCCGGCCCCCGTGGGGGGGCAGGGCACCGCCGATaat > 2:332933/1‑136 (MQ=255) aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGGGTGGCAGGCCAACCCCGATAACGCGCCGCTCg > 5:142559/1‑137 (MQ=255) aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCCCGCCTATAAGGAGCAGCTCGcg > 1:200000/1‑136 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCAGGGGGTGGCAGGCCACCCCCTCTAAGGCGCAGCTCGCTGATCa > 5:238487/1‑137 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCACGCCGATAAGGCGCAGCTCGCTGATCa > 7:183364/1‑137 (MQ=255) gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCCCCGCCGATAAGGCGCAGCTCGCGGATCAGGc > 3:270767/1‑137 (MQ=255) gcTCGAGGTCCTGCACTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCCCGCGGGCCGGCCCCGCGCAGCCCGGGGTTGGCAGGCAACCCCCGATAAGGCGCCGCGCGCCGAACatgg > 4:46594/1‑134 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCg > 1:8452/1‑137 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCCCTGCTGCTGGCCGCCCTCCCTGGCGGCGCCCGCGCAGCCTCGGGGTGGCAGCCCAACCACAATAAGGCGCAGCTGGCGGCTCAGGCCg > 7:122876/1‑137 (MQ=255) ggttGGGCCCCCCCGCGGGGCCGCGGTGGCGCGGGGGCGGCCCCCCAGGGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt < 6:186349/135‑1 (MQ=255) gcTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTGGCGGCGCTCGGGCCGCGGGGGTGTGGGAGGCCACCGCCGCGAAGGCGCCGGGCGCGGGTGCGGCCGCGGGCTTGGGCCAGCAGtt > 8:45053/1‑137 (MQ=255) gcTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGGCGGCGCCCGGGCGGCCGGGGTGTGGGAGGCCAACGCCGATAATGCGCAGCTCGCGGCTCATGCCGAAGGCTTCGGCcagcaggg > 6:108130/1‑135 (MQ=255) cGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCACCGCCTATAAGGCGCAGCTCGCGGATACGGCCGATGACGTCGGGAAGCAGTTGGCGGAGAAggt > 7:212699/1‑136 (MQ=255) gCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGGGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCAACGCCGATAAGGCGCCGCTCGCTGATCAGGCCGCTGCCGTCGGCCAGCAGGTTGCGGCGAAGGAt > 4:155221/1‑137 (MQ=255) ggcgcgGTGGTGCGGCGGCGGCCCCCCGGCGTGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc < 8:238092/135‑1 (MQ=255) ccgcgcTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGGGGATCAGGCCGCTGACTTCGGCAAGCAGTTGGCGGAGAACGATCGCGCGCCCCGGCGGGCGCGGGAGAAGGCTg > 6:211043/3‑137 (MQ=255) | CAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCA‑CCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTG > KT2440/3440226‑3440478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |