| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 6,039,837 | 0 | C | A | 28.4% | 72.6 / 23.3 | 67 | A37S (GCC→TCC) | puuD_3 | Gamma‑glutamyl‑gamma‑aminobutyrate hydrolasePuuD |
| Reads supporting (aligned to +/- strand): ref base C (16/32); new base A (0/19); total (16/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.14e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGG > KT2440/6039707‑6039965 | ggggCGCCTTTGTAATGAGGCGGCTGTATATTTGACGGCGCGCCGTTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGGGTGGCGCTGCCCCCATCCCGGGCCGGC‑AA‑CATCAGTGCCAAGCCGGAAAAgcc < 6:190296/137‑1 (MQ=255)ggggCGCATTTTTAATGATGCGGCTCGATATTTGACGGCGACCCGGTAAAAACAAGGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCAGCCCGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGAAAAgcc < 8:288721/137‑1 (MQ=255) ggCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAgccgg > 7:85060/1‑137 (MQ=255) gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAgccggc > 8:45873/1‑137 (MQ=255) gcgcCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAgccggc > 4:296048/1‑137 (MQ=255) ttGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTc > 8:314610/1‑137 (MQ=255) ttGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTc > 6:196208/1‑137 (MQ=255) ttGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTc > 3:745/1‑137 (MQ=255) tGTAATGAGGGGGCTCTATATTTGACGGCGCCCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCCGCGCTTGCGTGTCGCGGCCGCCAACACGGGCCGCC‑AA‑GATCAGTGCCAAGCCGGAAAAGCCGGCCGCCTCg < 2:154869/137‑1 (MQ=255) atgCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACt > 6:100856/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGt > 4:306060/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGt > 4:170655/1‑137 (MQ=255) cGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCAGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGt > 6:112770/1‑137 (MQ=255) cgcGATATTTGAAGGCGAGCCGGAAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCGGCCGCCATCACGGGCCGGC‑AA‑GAGAAGGGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGc < 7:74461/135‑1 (MQ=255) cTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGc > 8:320576/1‑137 (MQ=255) cGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCg > 1:260079/1‑137 (MQ=255) atTTGAAGGCGAGCCGTTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCg < 8:347444/137‑1 (MQ=255) tGACGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCAGCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCgtgt < 8:116982/137‑1 (MQ=255) acggCGCGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCGGCCGCCATCACGGGCCGCA‑AA‑GATCAGTGGAAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACTGTGTGc < 8:231685/135‑1 (MQ=255) aaGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGc < 7:314610/137‑1 (MQ=255) ccgcgCCGGTAAAAACAAGGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCgag < 5:101795/133‑1 (MQ=255) gggttAAAAACATTGCCGTGCAGGCGAGCCAGCAGCGCTTGGGTGTCGCTGCCGCCATCACGGGCCGGC‑AT‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGtt < 5:339890/133‑1 (MQ=255) ggTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGttct > 8:332474/1‑137 (MQ=255) aaaaCAATGCCGGGCAGGCGAGCCAGCCGCGCTTGCGTGGCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTccc < 4:240171/137‑1 (MQ=255) tGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgc < 3:170655/137‑1 (MQ=255) ggCCGTGCTGGCGAGCCAGCAGGGCTTGGGGGGCGCGGCCCCCATC‑CGGGGCGGCAAA‑CATCTGTGGCACGCCGCAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACctgc < 3:306060/136‑1 (MQ=255) tGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 7:320576/137‑1 (MQ=255) ggatggCGTGCCAGCAGCGCTTGCGTGGCGCTGCCGCCATCAGGGGCGGGC‑AA‑TAGCAGGGGCAACCGGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 7:324180/133‑1 (MQ=255) ggCAGGCGAGCCTGCAGCGCTGGCGTGGCGCTGCCGCCATCCCGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACg < 6:267359/136‑1 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa > 2:33389/1‑137 (MQ=255) aGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCa > 7:170796/1‑137 (MQ=255) gttGGGTGTCGGTGCCGCCATCACGGGCCGGC‑AA‑GTTCAGTGGCAAGCCGTCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATc < 8:228304/136‑1 (MQ=255) gtgtCGCGGCCGCAATCCCGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAgg < 1:100825/137‑1 (MQ=255) gcggccATCCGGGGCCGGA‑AA‑GTTCCTGGGCACGCGGGCAATGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt < 2:302582/134‑1 (MQ=255) gccgccATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt < 1:41469/137‑1 (MQ=255) cccgccATCAGGGGCCGGA‑AA‑GAGCAGTGGCACGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAt < 4:277766/136‑1 (MQ=255) cgcaATCAGGGGCGGGA‑AA‑TATCCGGGGCAAGCGGCAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCAttt < 3:56134/137‑1 (MQ=255) atccggggCCGGC‑AA‑TAGGAGTGCCACGCCGGCAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 2:337843/132‑1 (MQ=255) aTCCCGGGCCGGC‑AA‑GAGCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:290416/137‑1 (MQ=255) aTCACGGGCGGGC‑AA‑TATCCGTGGCATGCGGGAAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:112770/137‑1 (MQ=255) aTCACGGGCGGGA‑AA‑TATCAGTGGCAAGCCGGCAACGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:233697/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCTGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:195596/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:201337/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:45873/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:126418/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:155968/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 8:71147/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:202030/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 6:242837/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:339118/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 4:56668/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGTGCAAACCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:196208/137‑1 (MQ=255) aTCACGGGCCGGC‑AA‑GATCAGGGGAAAGCCGGCAAACCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 5:68096/137‑1 (MQ=255) aTCACGGGCCGGA‑AT‑TATCAGGGGCACGCCGGAAACGCGGCCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 3:19187/137‑1 (MQ=255) aTCACGGGCCGGA‑AA‑TATCAGTGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 1:81317/137‑1 (MQ=255) aTCACGGGCCGGA‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTgcgc < 7:39883/137‑1 (MQ=255) ggcGGC‑AATGATCAGTGCCAAGCCGTAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATgg < 1:128395/135‑1 (MQ=255) ggC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 5:56655/137‑1 (MQ=255) ggC‑AA‑GATCAGTGCCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 1:33152/137‑1 (MQ=255) gcc‑AA‑GATAAGTGGAAATCCGGCAAAGCGGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCtt < 8:313605/135‑1 (MQ=255) aa‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca < 8:209633/137‑1 (MQ=255) aa‑GATCAGGGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTaca < 8:85060/137‑1 (MQ=255) tCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 3:296048/137‑1 (MQ=255) tCAGTGCCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 7:172462/137‑1 (MQ=255) gcAGGGGCAAGCCGGAAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCgt < 5:91460/136‑1 (MQ=255) cAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg > 1:25443/1‑137 (MQ=255) aacgCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAgg < 6:55574/134‑1 (MQ=255) | GGGGCGCCATTGTAATGATGCGGCTCGATATTTGAAGGCGAGCCGGTAAAAACAATGCCGTGCAGGCGAGCCAGCAGCGCTTGCGTGTCGCTGCCGCCATCACGGGCCGGC‑AA‑GATCAGTGGCAAGCCGGCAAAGCCGGCCGCCTCGACATACTTGTCGCCGACCGTGTGCGAGGAGTTCTTCCCTACCTGCTGACGGCAGGCGCTGACACCGATCAAAGGGACCGCATTTGCGCTCATGGGCTTACACCGTGTGCAGG > KT2440/6039707‑6039965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |