| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 759,419 | A→C | 47.7% | intergenic (+28/‑140) | mmuM_1 → / → CJ019_00729 | Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 759,419 | 0 | A | C | 47.7% | ‑2.1 / 2.4 | 21 | intergenic (+28/‑140) | mmuM_1/CJ019_00729 | Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (7/3); new base C (8/2); total (16/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGC‑TTTTGCAGCGCCCGGCCCGCAG‑CCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCT > KT2440/759294‑759520 | gggCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGACACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGG‑GTTTGGCGCgccccgc > 5:206526/1‑135 (MQ=255) aaGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGG‑GTTTGGAGCGCCccgcccgcc > 5:276757/1‑134 (MQ=255) aCTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCACCCTGGCCTAAGCGCCCACGTGGCA‑CGGGGGC‑TTGTTTAGCCCCCGGCCCGcccgcc > 1:105258/1‑130 (MQ=255) cGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGC‑TTTTGCAGCGCCCGGCCCGCCG‑CCAttta > 8:9458/1‑132 (MQ=255) gTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGC‑TTTTTCCGCCCCCCGCCCCCCG‑CCCTTtaa > 3:246731/1‑134 (MQ=255) ggggTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGCGTTTTGC‑GCGCCCGGCCCCCAGCCCATTaaaaaaacg > 7:173847/1‑133 (MQ=255) ggggTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGC‑GTTTGCCGCGCCCGGCCCGCCG‑CCATTTAAAAAAACg > 3:319856/1‑135 (MQ=255) tCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCACCCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGG‑GTTTGCAGCGCCCCGCCCCGAG‑CCATTCACAAAACCGCCAGTCAGACCCCGa > 8:88409/1‑135 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGGGGC‑GGTTTGAGCGCCCCGCCCGCCG‑CCCATAAAAAAAACGCCCGGCAGACCCGGagt > 6:58977/1‑133 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGG‑TTTTTCCGCGCCCGGCCCGCCG‑GCATTTAAAAAAACGCCCGTGAGGCCCGGCtt > 5:151225/1‑135 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGG‑GTTTGCCGCGCCCGGCCCGCCG‑CCCGTAAAAAAAACGCCCGTCAGCCCCggcgt > 8:91940/1‑132 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGC‑GTTTTCCGCGCCCGGCCCGCCG‑CCATTAAAAAAAACGCCAGTCCGACCCGGAtt > 8:199044/1‑135 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGC‑GTTTGCAGCGCCCCGCCCGCCG‑CCATTAAAAAAAACGCCAGTCACCCCCGCAtt > 6:235300/1‑135 (MQ=255) cggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGG‑GTTTGGCGCGCCCGGCCCGGCG‑CCCTTAAAAAAAACGCCCGGGAGACCCGGGtt > 7:274046/1‑133 (MQ=255) ggAGCACATCGCGGTGCTTAAACGAAACCGGCCCTAACCGCCCCGGGGGCG‑GGGGGGC‑TTTGGCAGCGCCGGGCCCGCAG‑CCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTc < 7:199044/135‑1 (MQ=255) aagcgccgcGGTGAACCCCACCCTGGCCACCGCCCCCACGGGGCC‑GGGGGGT‑TTTGGCCGCCCCGGCCCCGCAG‑CCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTcc < 4:47884/132‑1 (MQ=255) gCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGG‑TTTTTCCGCGCCCGGGCCGCAG‑CCCTTAAAAAAAACCCCAGTCAGACCCGGATTCCCTCGTTTTTCAGGCCTCCGGCATCTcccga > 2:183618/1‑132 (MQ=255) gCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑CGGGCGC‑TTTTTCAGCGCCCCGCCCCCCC‑CCAATTAAAAAAAAGCCCGTCACACCCCGCTTGAATAGTTTTTAACGGCTGCGTCATATCCCgat > 4:162772/1‑135 (MQ=255) gCCTAAGCCCCCACGGGGCC‑GGGCGGG‑TTTTCCAGCGCCGGGCCCGCAG‑CCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgct < 6:301368/135‑1 (MQ=255) gCCTAACCGCCC‑CGGGGCCGGGGGGGG‑TTTGGCCGCCCCCGGCCCGCAG‑CCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgct < 4:216876/135‑1 (MQ=255) cccTACGCGCCCCCGGGGCG‑GGGGGGC‑TTTTCCAGCGCCCGGCCCACAG‑CCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgct < 8:173847/134‑1 (MQ=255) | GGGCCAAGGACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCACGTGGCC‑GGGCGGC‑TTTTGCAGCGCCCGGCCCGCAG‑CCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCT > KT2440/759294‑759520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |