| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,138,494 | T→G | 46.6% | intergenic (‑108/+33) | pigA_1 ← / ← hemR | Heme oxygenase PigA/Hemin receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,138,494 | 0 | T | G | 46.6% | ‑2.5 / 2.5 | 21 | intergenic (‑108/+33) | pigA_1/hemR | Heme oxygenase PigA/Hemin receptor |
| Reads supporting (aligned to +/- strand): ref base T (2/9); new base G (6/4); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
GGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCTCTTCGCGGGC‑GTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCG > KT2440/1138360‑1138623 | ggAGCGCAGGGCTGGGCGGTCGGTCAGGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCTCTTCGCGGGC‑Gt < 4:244143/135‑1 (MQ=255) cAGGGCTGGGCGGGCGGGAATGGGGGAGTCCTTGGAAAGAGGGCGGCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCTCTTCGCGGGC‑GTGCCCGc < 4:298950/135‑1 (MQ=255) cggggCGGTCGGTCATGGGGCAGTCCTTGGAAAGCGGCCGTCTACATACACTACGAAACAGCGGCAGCGACACAGTAAAAAACGCCGGTATTGGCGTGGCCAGTACC‑GGCCTCTTCGCGGGC‑GGGCCCGCAAaga < 2:258267/133‑1 (MQ=255) cgggcggtCATGGGGGAGGCCTTGGAAAGAGGGCGTCTACATACACCCCTAAACCGCGGGAGCGACACAGTAAAAAACGCCGGTATTTGCGTTGCCTGTCCC‑GGCCTCTTCGCGGGC‑GGGCCCGCAAAGAGGCCg < 4:143363/135‑1 (MQ=255) ggtcggtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCAGTTATCGGGC‑GGGGCCCCCAAAAGGcccg > 5:111518/1‑133 (MQ=255) gtcATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACCGGGCCACGACCCGCGG‑GGGCCCGCCAAAAGGCCgggccg > 6:3226/1‑131 (MQ=255) aGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCACGTTGCCGGG‑GGGGCCCCAAAAAGGCCGGGTCAGGCGGTACGcc > 8:171897/1‑135 (MQ=255) cTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCTCTTAGCGGGC‑GGGGCCGCAAAGAGGCCGGTACAGCCGTTACGcc > 1:99834/1‑131 (MQ=255) cTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCACTTCGCGGGC‑GGGGCCCCCAAGACGCCCGGACAGCCGGAACGCCGCa > 3:260760/1‑134 (MQ=255) aaaGAGGGCGTCTAGCTACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCTCTTCGCGGGC‑GTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATc < 7:224859/135‑1 (MQ=255) gcgggCGTCTTGATCCAAGACGAAACCGCGGGTGCGAGACTGTAAAAAACGCCGGTATTTGCGTTGCCTGTCCC‑GGCCTCTTCGCGGGC‑GTGCCCGAAACGAGGCCGGTACAGACGTAACGCCTCAGATATCCCa < 7:69167/133‑1 (MQ=255) gTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCGCTTCGCGGGC‑GTGGGCGCAAAGAGGGCGGGTCAGCCGTAAAGCCGCAGCTATCCCCAAccc > 2:195032/1‑133 (MQ=255) aCGAAACAGCGGGAGCGAGACAGAAAAAAACCCCGGTATTTGCGTTCCCCGTACC‑GGCCTCTACGCGGGC‑GGGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAg < 2:49260/135‑1 (MQ=255) gggAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCACTAAGCCCGC‑CTGGCCCCAAATCCGCCCGTTCAGAAGTAAAGCCCCACCTAAACCAACCCACAATTAAAACCAAAATTGcccccc > 8:154738/1‑135 (MQ=255) gAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCTCTTAGCGGGC‑GGGGCCGCAAAGAGGGCGGGACAGCCGGTACGCCGCAGATTTCCCAAACCAC‑ATTGGTACCGCAGTTGCGCCCCGGc > 4:85754/1‑135 (MQ=255) tATTTGCGTTGCCAGTACC‑GGCCTCTTCGCGGGC‑GTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggct < 4:260760/135‑1 (MQ=255) tATTTGCGTTGCCAGTACC‑GGCCTCTTCGCGGGC‑GTGCCCGCAAAGAGGCCGGTACAGACGTAACACCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTtggct < 3:257296/135‑1 (MQ=255) gCCAGTCCG‑GGCCGGTTCGCGGGC‑TGCCCCGCAAAGAGGCCGGTACAGAAGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca < 8:149634/135‑1 (MQ=255) gCCAGTCCC‑GGCCTCTCCGCGGGC‑GTGCCCGAAAAGCGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca < 3:51850/135‑1 (MQ=255) cctgtccc‑GGCC‑CTTAGGGGGCGGTGCCCGAAACCTGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTca < 7:154738/128‑1 (MQ=255) gcgGGC‑GTGCCCGCAACTAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCt < 6:184806/132‑1 (MQ=255) gggC‑GTGCCCGCAAAGTGGCCGGTACATACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTTGCTGGCTGGGTCACAGCCGCCTCACCCTGGCcg < 7:216223/135‑1 (MQ=255) | GGAGCGCAGGGCTGGGCGGTCGGTCATGGGGGAGTCCTTGGAAAGAGGGCGTCTAGATACAAGACGAAACAGCGGGAGCGAGACAGTAAAAAAAGCCGGTATTTGCGTTGCCAGTACC‑GGCCTCTTCGCGGGC‑GTGCCCGCAAAGAGGCCGGTACAGACGTAACGCCTCAGATATCCCAAACCAC‑ATTGATACCGAAGTTGCGCCCCGGCATGGTCAGGCGGTCGATGTTGGCTGGCTGGGTCACAGCCGCCTCACCCTGGCCG > KT2440/1138360‑1138623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |