| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,289,845 | T→G | 46.2% | intergenic (+119/‑106) | CJ019_04021 → / → gloA | hypothetical protein/Lactoylglutathione lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,289,845 | 0 | T | G | 46.2% | 4.2 / 2.3 | 25 | intergenic (+119/‑106) | CJ019_04021/gloA | hypothetical protein/Lactoylglutathione lyase |
| Reads supporting (aligned to +/- strand): ref base T (4/6); new base G (4/6); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAA > KT2440/4289715‑4289971 | tGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCACCGCCCGGCCt > 7:81245/1‑135 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGGGCCCGGccggttga > 5:245821/1‑129 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGGGCCCAGCCgtttga > 3:131236/1‑132 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCATTGCa > 7:7392/1‑135 (MQ=255) aCGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCTTTGCa > 3:22639/1‑135 (MQ=255) ccACATAGGCTTCACAGTTTTTAACAAAGGAGTGGCTCAGTACACAAACAGAAAACGCACTCGCCACCCCATCCCGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTg < 8:311898/135‑1 (MQ=255) acaTAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCGTTCATTTgg > 4:132700/1‑133 (MQ=255) acaTAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCCCCCCTCCTTTTCACGGGGCGTTAAttttg > 1:9832/1‑132 (MQ=255) tccaaacTTTTTTCAAACGCCCTGCCTCTTTATCGAACCAGCTAAAGGCCTACCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTCCAGGGCGCTTTCATTTGCGCAATCGgc < 8:99204/130‑1 (MQ=255) acacTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCAATTTCGCAATCGGCG‑Ca > 4:72632/1‑135 (MQ=255) gCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGGCTTTGCAGGGGGGTTTGAATTGGGCAATCCGCG‑GACTGCAGTATAATGGCCAGccg > 6:326763/1‑134 (MQ=255) cAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGTGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCgg > 7:302280/1‑135 (MQ=255) aaaGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCAGCCTTTGCAGGGCGCGGTAATTTGAGCAATCGGAG‑CACGGCCGGATCATGGCCCGCCTGTCTGTTTTTCCGGCGccc > 5:158538/1‑135 (MQ=255) cgcccccccAGCCCGCG‑CCCCCACTGGGGGGCATATATTTTCTCGGCCGGGCTTTTGCGGGCGGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCg < 8:7392/130‑1 (MQ=255) ccgccccAACCCGCGCCCCGCACTTCCGGGCGTATTATTACCCCCCCCGGCATTTCCTGGGCGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCgg < 3:132700/132‑1 (MQ=255) aTCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCGGCCGTTGCAGGGCGCGTTGATTTTGGCAATCGGCG‑CCCGGCCGGGTACTGGCCCGGCGGGGGGTTTTTCCGGCGGCCGGCGCCCGGCCCCggcg > 4:218432/1‑132 (MQ=255) cACGCG‑CCCCAAATTGCGGGCAGATAATTCCCGCGCCCTGCCTTTGAGGGGGGGTTTCATTTGCGCAATCGGCT‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCGGGCGCCCTGCGCCCGGCCCCGTCTCa < 4:131236/135‑1 (MQ=255) ccggg‑cCCGCAAATGCGGGCCTGTAATTTCAGGGCCCGGGCTTTGCCGGGGGCTTTCATTTTCGCAATCGGCGCCACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCa < 4:22639/131‑1 (MQ=255) ccccgccccGCACTGGCGGGCCTCAAATATCCCCCCCGGGCTTTTCCAGGGCGCTTTCATTTGCGCAAGCGGCG‑CACTGCCGTATCATGGCCAGCCGGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCa < 2:15469/129‑1 (MQ=255) cgcg‑CCCGAAATTGCGGGCCTCTAATTTCCGCGCCCTGCCTTTGCTGGGCGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAgg < 2:187589/135‑1 (MQ=255) cGCACATGCGGGCCTCTAATTTCAGCCCCCCGCCATTTCCCGGCGCGATCATTTTCGGAAACAGCG‑CGCAGCCGTATCATGGCCCGCCAGGCGGTTTTTACCGCGCCCAGCCCCCCGCCCCGGCGCACGAggtac > 2:52649/1‑131 (MQ=255) ggCGTCAAATTGCAGCGCCCTGCGTTTGCAGGGCGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGGCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTg < 7:283138/135‑1 (MQ=255) aaTTCCCGCGCCCGGCCTTTCCCGGGCGCTTTCATTTGCGCAAGCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATc < 6:105844/135‑1 (MQ=255) cccgccccTCCATTTGCAGGGGGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTGGCGGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCa < 6:245821/129‑1 (MQ=255) cgcgcCCGGCCTTGGCAGGGGGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCaa < 2:9832/133‑1 (MQ=255) | TGGGTACGCTGATGAAATGCCCACATAGGCTTCACACTTTTTTCACAAGGACTGGCTCAGTATCGAAACAGCTAAAGGACTAGCGACCCCATCACGCG‑CCCGCACATGCGGGCCTCTAATTTCAGCGCCCTGCCTTTGCAGGGCGCTTTCATTTGCGCAATCGGCG‑CACTGCCGTATCATGGCCAGCCTGTCTGTTTTGCCGGCGCCCTGCGCCCGGCCCCGTCTCAGGAGTTCCCATGAGCCTGCACGATCTGCAA > KT2440/4289715‑4289971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |