Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	120,983	(T)9→8	intergenic (+280/+92)	polA → / ← engB	DNA polymerase I/putative GTP‑binding protein EngB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	120,975	0	T	.	100.0%	174.6 / NA	38	intergenic (+272/+100)	polA/engB	DNA polymerase I/putative GTP‑binding protein EngB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (28/10);  total (28/10)

TAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCCAGT  >  KT2440/120848‑121109                                                                                                                                |                                                                                                                                        tAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGtttttttt                                                                                                                                  <  4:308134/135‑1 (MQ=255)  aaCTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGGTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGttttttttg                                                                                                                                 >  8:238689/1‑134 (MQ=255)              ggTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGc                                                                                                                    >  8:167110/1‑135 (MQ=255)                  aaGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCg                                                                                                                  >  7:257811/1‑133 (MQ=255)                            aTGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAACTCCGGGG‑TTTTTTTTGCCCGGAATTTGCAGGGACCCGGtttt                                                                                                      >  5:255887/1‑135 (MQ=255)                                      gttgtGTTCAGTGTTGTCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACATCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                             <  8:232460/134‑1 (MQ=255)                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGACTTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                      >  3:156654/1‑134 (MQ=255)                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                      >  1:310595/1‑134 (MQ=255)                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                      >  8:266248/1‑134 (MQ=255)                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt                                                                                      >  6:98591/1‑134 (MQ=255)                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑GTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAGTCGATGT‑c                                                                                    >  5:188101/1‑135 (MQ=255)                                               aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCGCGCGCATGCGATGT‑Cgc                                                                                  >  5:65970/1‑134 (MQ=255)                                                  gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGCGCGGGTTTTCTCGCGAATGCGATGT‑CGAAt                                                                                >  5:144685/1‑134 (MQ=255)                                                    tGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATaca                                                                             >  2:21706/1‑135 (MQ=255)                                                       cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                             >  2:322226/1‑117 (MQ=255)                                                       cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                             <  1:322226/117‑1 (MQ=255)                                                              cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAACTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCAtt                                                                   >  2:266322/1‑135 (MQ=255)                                                                       gCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTaa                                                          <  7:51831/135‑1 (MQ=255)                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc                                                                                           >  7:18397/1‑101 (MQ=255)                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc                                                                                           <  8:18397/101‑1 (MQ=255)                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAAc                                                        >  4:125799/1‑135 (MQ=255)                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAAc                                                        >  4:67828/1‑135 (MQ=255)                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCGCGCGACTGGGAGGT‑CGCATACACCCCCGCCTTCGCGGGGAAAc                                                        >  1:159395/1‑135 (MQ=255)                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCAGGGG‑TTTTTTTTGCCCGGAATTTGGCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGATAACACCGCCGCATTCGCGGGTAAAc                                                        >  4:159934/1‑135 (MQ=255)                                                                              cGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCg                                                     <  3:260989/133‑1 (MQ=255)                                                                                   cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAgg                                              >  8:132871/1‑135 (MQ=255)                                                                                    ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                             <  3:67828/135‑1 (MQ=255)                                                                                    ggACTTGGACCCCGAACTTCCCCCGCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                             <  3:222552/135‑1 (MQ=255)                                                                                        ttGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCaa                                         >  8:58075/1‑135 (MQ=255)                                                                                        ttGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCaa                                         >  8:58073/1‑135 (MQ=255)                                                                                             ccccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTGACCCCGGGTCCGGCGCAACCCAc                                    >  3:83645/1‑135 (MQ=255)                                                                                             ccccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCa                                     >  7:167880/1‑134 (MQ=255)                                                                                              cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGcc                                                                                                                   <  7:164913/56‑1 (MQ=255)                                                                                              cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGcc                                                                                                                   >  8:164913/1‑56 (MQ=255)                                                                                              cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCAc                                    >  7:105086/1‑134 (MQ=255)                                                                                                   aCTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACg                              >  6:314018/1‑135 (MQ=255)                                                                                                               ccATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCt                  >  7:115057/1‑135 (MQ=255)                                                                                                                cATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCtc                 >  5:35149/1‑134 (MQ=255)                                                                                                                  tACGATGTCCGGGG‑TTTTTTTTGCCGGTAATTTGCCGGGAGCGGGTTTTCGCGCTAATGCGATGT‑CGAATACCCCGCCGCATTCGCGGGTAAACCCGGGGCAGGCGAAAACCACTACACGACCGGCTTGTCTTcc               <  6:102446/135‑1 (MQ=255)                                                                                                                   aCGAAGTCCGGGG‑TTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCa              <  4:293714/135‑1 (MQ=255)                                                                                                                            cggggTTTTTTTTGCCCGGAATTTGCCGGGAGGGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGGGGGTAAACCCGGGTCAGGCGCAAACCACTACACGACCGGCTTGTCTTCCAGTtccatc      <  8:175982/134‑1 (MQ=255)                                                                                                                                ttttttttgGCCCGTATTTTGCGGGTCGGGGGTTTTGTCGC‑TATGGGTTTTACTAAAACCCCCCCGAATTCGGGGTAAAACCGGGGTATGGCGAAAACAAATAAACGACCGGCTTGTCTTCCAGTTCCATCCAGt  <  3:324000/135‑1 (MQ=255)                                                                                                                                |                                                                                                                                        TAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGT‑CGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATCCAGT  >  KT2440/120848‑121109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑