| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,875,884 | 0 | A | C | 26.4% | 162.5 / 13.4 | 91 | N300H (AAC→CAC) | ttgI_2 | Toluene efflux pump outer membrane protein TtgI |
| Reads supporting (aligned to +/- strand): ref base A (10/57); new base C (24/0); total (34/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.19e-14 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTG > KT2440/3875753‑3876017 | tCAGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑ACg > 8:229432/1‑135 (MQ=255) aGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑ACGtt > 6:13598/1‑135 (MQ=255) aGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCACGCCC‑ACCtt > 1:39188/1‑135 (MQ=255) aGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCAGGGTGAGCTGCTGCGCCGCCGCCCGGCCATCCGCAGCGCAGAGCGCCAGCTGGCGGC‑GGCCACCGCCa‑aactt > 6:265342/1‑131 (MQ=255) gCGTAGACCTGGCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGtt < 2:211494/134‑1 (MQ=255) tGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGCGGGCCCCCCCCC‑CCGTTGGG‑GGGGcc > 2:219664/1‑135 (MQ=255) tGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCCCCC‑ACCGTGGG‑GGGGCCa > 2:81877/1‑135 (MQ=255) tGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCCCCC‑ACGTTGTC‑GTGGCCa > 1:40602/1‑135 (MQ=255) cGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCCCCC‑ACCATGGC‑GTgggccccc > 6:104870/1‑129 (MQ=255) cccACGGCCCTGCCGGCCATCGCCAAGGCGCTGCGGGTCGGTGCCCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCTAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCAccgcc < 8:168438/135‑1 (MQ=255) cccACGGCCCGGCCGGCCAGCGCCCCGGCGCTCCCGGGCGGTCACCCGGGTGCGCTGCGGCGCCGCCGCCCGGCCAGCCGCCGCGCCGAGGGCCCGGGGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCAccgcc < 2:99760/135‑1 (MQ=255) cccAAGGCCCTGCCGGCTATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑ACCTTGGC‑GTGGCCAccccc > 1:89166/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGCCCCGGGTGCGCTGCTGCGCCGCCGCCCGGACATCCGCCGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑CCGGTGGC‑GGGGCcccccgc > 5:40228/1‑132 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCTGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGCGCGCCAGCTGGCGGC‑CGCCCCCGCCACCCGTCGGC‑‑TGGCCCccgcc > 3:75931/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGCGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑CCGGTGGG‑CTGGGCCccgcc > 3:106662/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCGCCC‑ACCTTGGC‑GTGGCCAccccc > 8:246656/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCCCCC‑ACCGTGGC‑GGGGcccccccc > 5:161702/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑ACGTTGGC‑GTGGCCCccgcc > 4:63414/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑ACGTTGGC‑GTGGCCAccgcc > 7:89601/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑ACGGTGGC‑GGGGcccccccc > 7:68977/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑CCGTTGGC‑GTGGCCCccgcc > 4:108537/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑ACGTTGGC‑GTGGCCAccgcc > 4:152631/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑ACGTTGGC‑GTGGCCAccgcc > 4:62931/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑ACGTGGGC‑GTGGcccccccc > 7:258728/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑ACCTTGGC‑GTGGCCCccgcc > 3:155440/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCCCCC‑CCGTTGGG‑GTGGCCCccgcc > 6:94602/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCC‑ACGTTGGC‑GTGGCCAccgcc > 4:327318/1‑135 (MQ=255) cccAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCAccgcc < 8:255630/135‑1 (MQ=255) ccTGCCGGCCAGCGCCACGGCGCGGCCGGGCGGTGCCCCGGGTGAGCTGCGGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCGGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGtt < 5:239048/135‑1 (MQ=255) tGCCGGCCATCGCCACGGCGCGGCCGGGCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTcc < 4:168075/135‑1 (MQ=255) cGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑CCGTTGGC‑GGGGCCCCCGCCGCCCGGGTCCcgc > 6:55603/1‑135 (MQ=255) cGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑ACCGTGGC‑GTGGGCCCCCCCCCCCAGTTCCcgc > 2:40333/1‑135 (MQ=255) ggCCATCGCCACGGCGCTGCCGGTCGGTGACCCGGGTGCGCTGCGGCGCCGCCGCCCGGACAGCCGCCGCGCCGAGCGCCCGCTGGCGGC‑GGCCACCGCCA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCcgcg < 1:71056/135‑1 (MQ=255) ggCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGCCATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑CCCGTGGG‑GTGGCCCCCCCCGCCCCGGTCCcccg > 8:142193/1‑135 (MQ=255) gCCATCGCCATGGCGCTGCCGGGCGGTGACCCGGGTGAGGTGCTGCGCCGCCGCCGGGACATCGGCAGCGCCGTGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCcgcgc < 4:54497/135‑1 (MQ=255) gCCAGCGCCAGGGCGCGGCGGGGCGGGGCCCCGGGGGCGCGGCGGCGCCGCCGCCCGGACACCCGCCGCGCCGAGCGCCCGCGGGCGGC‑GGCCACCGCCA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCcgcgc < 1:219664/135‑1 (MQ=255) cGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑ACGTTGGC‑GTGGCCACCGCCGCCCTGTTACCGCGCGTCAg > 2:49622/1‑135 (MQ=255) aGGCGCGGCCGGTCGGTGACCCGGGGGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCa < 6:73803/135‑1 (MQ=255) ccGGTCGGTGCCCCGGGTGAGCTGCTCGGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGGTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTc < 4:103657/135‑1 (MQ=255) ccGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑ACCTTGGCGGTGGCC‑CCGCCGCCCGGTTGCCGCGCCTCCGCCGCAGTggggtc > 8:60590/1‑131 (MQ=255) gggcgggGACCGGGGTGAGCTGCGGCGCCGCCGCCCGGACATCCGGAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCt < 7:250109/132‑1 (MQ=255) ggTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCC‑CCCTTTGC‑GTGGCCCCCCCCGCCCCGGTGCCGCGCCTCAGCCCCAGTGGCGTCCGCGGCGTTAccccc > 8:164672/1‑135 (MQ=255) tGAGCTGCGGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGGCAGCCTCAGTGGCTTCCTCGGCTTTACcgccgc < 4:137559/135‑1 (MQ=255) ggcgcggctgcGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCGTGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACcgccgc < 8:329091/129‑1 (MQ=255) gAGCTGCTGCGCCGCCGCCCGGACATCCGCCGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCgccgcc < 3:51664/135‑1 (MQ=255) gccgccgccCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCGCCC‑ACGTTGGC‑GTGGCCCCCGCCGCCCGGGTCCCGCGCGGCAGCCGCCGTGGCGTCCTCGGCTGTACCGCCGCCCGCGgcgcgc > 3:221690/1‑135 (MQ=255) ccgccgccCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCa < 7:80793/135‑1 (MQ=255) ccgccgccCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCa < 7:239629/135‑1 (MQ=255) aCATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCtt < 1:20070/135‑1 (MQ=255) cgccgcgccgCGGGCCCGGTGGCGGG‑GGCCACCGCCA‑ACGTTGGG‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTcggcg < 1:140517/135‑1 (MQ=255) cGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTcggcg < 3:221434/135‑1 (MQ=255) cGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTcggcg < 7:164672/135‑1 (MQ=255) gCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑ACGTTGGC‑GTGGCCACCGCCGCCCTGTTCCCGCGCGTCAGCCTCAGTGGCGTCCTCGGCTTTACCGCCGCCCGCGGCGCGCAGCTCGGCTCGTcggcgg > 8:171269/1‑135 (MQ=255) ccgcgcggcgcgCCAGCTGGCGGC‑GGCCACCGCCA‑CCGTGGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCggcggc < 5:13598/133‑1 (MQ=255) cAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCggcggc < 3:166697/135‑1 (MQ=255) cAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCggcggc < 2:4368/135‑1 (MQ=255) aGCGCCGAGCGCCAGCTGGCGGC‑GGGCCCCGCCC‑ACGTTGGC‑GTGGCCCCCGCCGCCCGGTTCCCGCGCGTCAGCCGCAGGGGCTTCCTCGGGGTTACCGCCGCCCGCGGCGCGCCGCTCGGCGCTTCGGCGGcc > 4:110445/1‑135 (MQ=255) cgcCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGGTGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCaa < 3:276724/135‑1 (MQ=255) ccccGAGCGCCAGCTGGCGGC‑GGCCCCCGCCA‑CCGTGGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCaa < 6:37126/133‑1 (MQ=255) gcCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAAc < 3:72462/135‑1 (MQ=255) cggAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACg < 1:81877/133‑1 (MQ=255) cGAGCGCCCGCGGGCGGC‑GGCCACCGCCA‑ACGGTGGC‑GGGGCCACCGCCGACCTGGTCCCGCGCGGCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGc < 5:97400/135‑1 (MQ=255) cgcCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTCCCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTgg < 4:116184/135‑1 (MQ=255) gcCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTggg < 8:326350/135‑1 (MQ=255) gcCAGCTGGCGGC‑GGCCACCGCCA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTggg < 5:103814/135‑1 (MQ=255) cccAGCTGGCGCC‑GGCCACTGCCA‑TCGGTCGC‑GTGGCGACGGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTggg < 1:174479/134‑1 (MQ=255) ccAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGc < 2:31140/135‑1 (MQ=255) cAGCTGGCGGC‑GGCCACCGCCA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCTGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCa < 2:290355/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑TCGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 3:112625/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCTGCCAACGCCTGGGCACTgg < 5:147198/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 6:174399/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 8:141477/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 2:39188/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 3:234069/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 8:192641/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 7:154912/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCCA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 5:55603/135‑1 (MQ=255) tGGCGGC‑GGCCACCGCAA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCGCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 7:44953/135‑1 (MQ=255) gggcggc‑ggcCACCGCCA‑AGGTTGGC‑GGGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTgg < 3:64305/134‑1 (MQ=255) ggcggc‑ggcCACCGCCA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTggg < 4:132051/135‑1 (MQ=255) ggc‑ggcCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGccc < 1:40333/135‑1 (MQ=255) c‑ggcCACCGCCA‑ACGTTGGT‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCCACGCCTGGGCACTGGGCCCgc > 4:31769/1‑134 (MQ=255) ggcCCCCGCCC‑ACGTTGGC‑GTGGCCCCCGCCGCCCGGTTACCCCGCGTCACCCGCAGTGGCGTACTCGGCTTTACCGCCGCCCGCGGCTGGCAGAACCGCTCTTCGGCGGCGCACGCCGCGGCCCTGGGGCCgcg > 8:84668/1‑133 (MQ=255) ccA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCt < 6:84185/135‑1 (MQ=255) ccA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCt < 8:88785/135‑1 (MQ=255) ccA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCt < 7:246656/135‑1 (MQ=255) ccA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCTCTGGGCCCGAGCATTACCt < 5:161407/135‑1 (MQ=255) cA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTg < 4:281292/135‑1 (MQ=255) cA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTg < 8:115545/135‑1 (MQ=255) cA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTg < 4:319989/135‑1 (MQ=255) cA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTg < 2:169559/135‑1 (MQ=255) cA‑ACGTGGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCGTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTg < 4:75931/135‑1 (MQ=255) | TCAGCGTAGACCTGTCGCCCAAGGCCCTGCCGGCCATCGCCAAGGCGCTGCCGGTCGGTGACCCGGGTGAGCTGCTGCGCCGCCGCCCGGACATCCGCAGCGCCGAGCGCCAGCTGGCGGC‑GGCCACCGCCA‑ACGTTGGC‑GTGGCCACCGCCGACCTGTTCCCGCGCGTCAGCCTCAGTGGCTTCCTCGGCTTTACCGCCGCCCGCGGCTCGCAGATCGGCTCTTCGGCGGCCAACGCCTGGGCACTGGGCCCGAGCATTACCTG > KT2440/3875753‑3876017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |