Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1340AC32.6% 37.7 / 10.8 49L182V (TTG→GTG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (22/11);  new base C (1/15);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 5.85e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245010‑4245265
                                                                                                                            |                                                                                                                                    
cGGGCGCCGTCGCGTATCCGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCACCGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑cc                                                                                                                           >  7:249697/1‑135 (MQ=255)
cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCCGTTCGGCGGCCACGGCAGGCCACTGCTCGTCGGCGCCGACGTCGCTGCCCAACCCCGCGG‑cc                                                                                                                           >  8:289865/1‑135 (MQ=255)
cGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑cc                                                                                                                           >  5:125079/1‑135 (MQ=255)
  ggCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCACCGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCAACCCGGCGG‑cccc                                                                                                                         >  7:255586/1‑133 (MQ=255)
      ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGGCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCCGCGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCTGACCAACCCGGCGG‑CCCGGgcg                                                                                                                     >  6:107173/1‑135 (MQ=255)
      ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCTGACCAACCCGGCGG‑CCCGGgcg                                                                                                                     >  7:106255/1‑135 (MQ=255)
      ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCAACCCGGCGG‑CCCGGgcg                                                                                                                     >  2:35307/1‑135 (MQ=255)
          cgcgTATCAGCCGCTTGGTCTGCGGATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCCGGCAACGGCTCGTCGGCCCAGCCTTCGCTGACCAACCCCGCGG‑CCCGGGGGCGAt                                                                                                                 >  8:109105/1‑135 (MQ=255)
                          ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCACGGCGCGATCCGCCGCCCACAAcac                                                                                                 >  4:187742/1‑133 (MQ=255)
                                 gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCAGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCCGGCGCGATCGGCGCCCCACAACTCGGCGacg                                                                                          >  3:39658/1‑133 (MQ=255)
                                     cggCGAAGGGCGGGCCATTGGCCTGGCGCCGGGCCAGTGCGGCGGCAACGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  7:99490/134‑1 (MQ=255)
                                        cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGCTCGGCGCCCCCCCACTCCGCGAGGacccgcc                                                                                   >  7:50670/1‑129 (MQ=255)
                                          aggggcGGGCAATTGGCCAGGCGCCGGGCCAGTGGGGCGGCAACGGCAGGCAACGGAGGGGCGGCGAATAATGCGCTCACCAACCCGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  2:69538/130‑1 (MQ=255)
                                               cGGCCCATTGGCCTGGCGCCCGGACAGTTGGGCGGAAACGGCAGGCAACTGCTCGGCGGCGCATACTTCGCGGACCAACCCGGCGG‑CCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                            <  6:139921/135‑1 (MQ=255)
                                                  gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCCGGCGCGCTCGGCGCCCCCCCACGCGGCGAGGGACACCAGgcgcgttgc                                                                         >  3:267271/1‑130 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCCACCCGGCGG‑CCCGGGCGCGCGCGGCGCCCCCCACCTCGGGGGCGGACCGCAGGCGCCTGGccg                                                                       >  6:139527/1‑134 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCCCCACGCCGCGAGGAAAACCCGGCGCGTTGCCt                                                                       >  2:27266/1‑135 (MQ=255)
                                                    cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGAAAGGCGCCCCCCCACTCGGCGCGGAACACCCCGCGCGTGGCCt                                                                       >  1:189099/1‑135 (MQ=255)
                                                         ccctggCGCCGGGCCAGTTCGGCGGCAAGGGCAGGAAACTGCGCGTCGGCGCAGACTTCGCTCACAAACCCGGCGG‑CCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  3:288872/131‑1 (MQ=255)
                                                         ccccggCGCCGGGCCTGGGCGGCGGCACGGGCCGCCAGCGGCCCGGCGGCGCAGAGTGCGCTCCCAACCCCGGCGC‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  2:282034/131‑1 (MQ=255)
                                                              gcgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTGGGCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  6:43702/135‑1 (MQ=255)
                                                              gcgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  7:197677/135‑1 (MQ=255)
                                                              gcgcCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGCCCACCCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  8:50670/135‑1 (MQ=255)
                                                              gcgcCCGGCATGTGCGGCGGAACCGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCGCACCACCCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  4:135133/135‑1 (MQ=255)
                                                                 cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCCGGGCGCGATCGGCGCCCCCCCACGCGGCGAGGAACAGCAGGCGCGTGGCCTGCGCGCAGCCGa                                                           >  8:226363/1‑134 (MQ=255)
                                                                    ggCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCCGGCGCGCTCGGCGCCCCCCCACACGGCGAGGCAAAGCACGCGCTTGGCCAGCGCCCAGCCgcgccg                                                       >  6:63888/1‑130 (MQ=255)
                                                                        cgtgcggcggCAACGGCAGCCAACGGCGCGGCGGCGCAGACTGCGCTGACCAACCCGGCGG‑CCTGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  6:130369/131‑1 (MQ=255)
                                                                        actgcggcggCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  4:111858/131‑1 (MQ=255)
                                                                                   aaCGCCAGGAAACTCCGGGTCGGCGCAGACTTCGCTCCCAAACCCGGGGG‑CCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                        <  7:200070/135‑1 (MQ=255)
                                                                                       gccggcaACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCA‑GCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTgg                                   <  2:37703/132‑1 (MQ=255)
                                                                                       gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGTGGCAGGTGCCCGCTg                                    >  8:202906/1‑135 (MQ=255)
                                                                                              aCTGCGCGGCGCCCCAGACTGCGCTCCACACCCCGGCGC‑CCGGGGCGCGCGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                             <  6:1341/135‑1 (MQ=255)
                                                                                                tGCTCGTCGGCGCAGACTGCGCTGCCCAACCCGGCGC‑CCAGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:162485/135‑1 (MQ=255)
                                                                                                tGCGCGGCGGCGCAGACTGCGCCCACCACCCCGGCGC‑CCCGGCCGCGACCGCCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:308195/135‑1 (MQ=255)
                                                                                                gggtcggcgGCGCCCACTGCGCTGACCACCCCGGCGG‑CCCGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTGGGCGTGCTCGCTGCCGATCAGCCGGGGCAGGTGCCCGCTGGCGCCGGCa                           <  2:38065/132‑1 (MQ=255)
                                                                                                     gtcgGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCCCAACGCGGCGAGGGACAGCAGGCGCTTGGCCGGCTCGCGGCCGCTCCGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCggg                      >  4:112908/1‑135 (MQ=255)
                                                                                                     gggggCGCTGACGTCGCTTCCCACCCGGGGGG‑CCTGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  3:253841/132‑1 (MQ=255)
                                                                                                          cgcAGACTTCGCTCCCCACCCCGGCGG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  6:67571/135‑1 (MQ=255)
                                                                                                          cgcAGACTTCGCGCACCAACCCGGCGG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  3:130413/135‑1 (MQ=255)
                                                                                                             aGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCGCGCTGCCGATCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGCTGTAGc              >  7:317465/1‑135 (MQ=255)
                                                                                                             aGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCCCCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGGGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCGGTAGc              >  4:282886/1‑135 (MQ=255)
                                                                                                             aGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGc              >  8:77876/1‑135 (MQ=255)
                                                                                                                       gACCAACCCGGCGG‑CCACGGCGCGATCCGCGCCCCACAACTCGTCGAGGAACAGCACGCGCTTGGCCTGCTCCCAGCCCATCAGCCGTGGCAGGTGCCAGCTGGCCCCGGCATAGGGGCTGTAGCCCAAGCTGgt    >  6:247881/1‑135 (MQ=255)
                                                                                                                         ccACCCGGGGGG‑CCTGGGCGCTTTGGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:109105/135‑1 (MQ=255)
                                                                                                                         ccACCCGGGCGG‑CCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:148947/135‑1 (MQ=255)
                                                                                                                         ccACCCGGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:133327/135‑1 (MQ=255)
                                                                                                                         ccACCCCGGCGG‑CCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:77876/135‑1 (MQ=255)
                                                                                                                         ccAACCGGGCGG‑CCTGGGCGGGAGGGGCGCCACACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCGGGCGCCGGCATCTGGGCTGTAGCCCATGCTGgtgt  <  4:241428/135‑1 (MQ=255)
                                                                                                                         ccAACCGGGCGG‑CCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:5839/135‑1 (MQ=255)
                                                                                                                          ccaCCCGGCGGCCCCGGGGGCCTGCGGCCCCCCACACCTCGTCGCGGAACAGCAGGCGCGTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:36371/133‑1 (MQ=255)
                                                                                                                            |                                                                                                                                    
CGGGCGCCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245010‑4245265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑