Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,245,1450AC56.4% ‑4.6 / 15.8 46L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (6/11);  new base C (13/9);  total (19/27)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245016‑4245265
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ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGGCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCCGCGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCTGACCAACCCGGCGG‑CCCGGgcg                                                                                                                     >  6:107173/1‑135 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCCGCCTTCGCTGACCAACCCGGCGG‑CCCGGgcg                                                                                                                     >  7:106255/1‑135 (MQ=255)
ccGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCAACCCGGCGG‑CCCGGgcg                                                                                                                     >  2:35307/1‑135 (MQ=255)
    cgcgTATCAGCCGCTTGGTCTGCGGATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCCGGCAACGGCTCGTCGGCCCAGCCTTCGCTGACCAACCCCGCGG‑CCCGGGGGCGAt                                                                                                                 >  8:109105/1‑135 (MQ=255)
                    ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCACGGCGCGATCCGCCGCCCACAAcac                                                                                                 >  4:187742/1‑133 (MQ=255)
                           gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCAGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCCGGCGCGATCGGCGCCCCACAACTCGGCGacg                                                                                          >  3:39658/1‑133 (MQ=255)
                               cggCGAAGGGCGGGCCATTGGCCTGGCGCCGGGCCAGTGCGGCGGCAACGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                      <  7:99490/134‑1 (MQ=255)
                                  cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCCACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGCTCGGCGCCCCCCCACTCCGCGAGGacccgcc                                                                                   >  7:50670/1‑129 (MQ=255)
                                    aggggcGGGCAATTGGCCAGGCGCCGGGCCAGTGGGGCGGCAACGGCAGGCAACGGAGGGGCGGCGAATAATGCGCTCACCAACCCGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAgg                                                                                 <  2:69538/130‑1 (MQ=255)
                                         cGGCCCATTGGCCTGGCGCCCGGACAGTTGGGCGGAAACGGCAGGCAACTGCTCGGCGGCGCATACTTCGCGGACCAACCCGGCGG‑CCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCtt                                                                            <  6:139921/135‑1 (MQ=255)
                                            gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCCGGCGCGCTCGGCGCCCCCCCACGCGGCGAGGGACACCAGgcgcgttgc                                                                         >  3:267271/1‑130 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCCACCCGGCGG‑CCCGGGCGCGCGCGGCGCCCCCCACCTCGGGGGCGGACCGCAGGCGCCTGGccg                                                                       >  6:139527/1‑134 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCCCCACGCCGCGAGGAAAACCCGGCGCGTTGCCt                                                                       >  2:27266/1‑135 (MQ=255)
                                              cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGAAAGGCGCCCCCCCACTCGGCGCGGAACACCCCGCGCGTGGCCt                                                                       >  1:189099/1‑135 (MQ=255)
                                                   ccctggCGCCGGGCCAGTTCGGCGGCAAGGGCAGGAAACTGCGCGTCGGCGCAGACTTCGCTCACAAACCCGGCGG‑CCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  3:288872/131‑1 (MQ=255)
                                                   ccccggCGCCGGGCCTGGGCGGCGGCACGGGCCGCCAGCGGCCCGGCGGCGCAGAGTGCGCTCCCAACCCCGGCGC‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCg                                                                  <  2:282034/131‑1 (MQ=255)
                                                        gcgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTGGGCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  6:43702/135‑1 (MQ=255)
                                                        gcgcCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  7:197677/135‑1 (MQ=255)
                                                        gcgcCCGGCCAGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGCCCACCCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  8:50670/135‑1 (MQ=255)
                                                        gcgcCCGGCATGTGCGGCGGAACCGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCGCACCACCCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGcc                                                             <  4:135133/135‑1 (MQ=255)
                                                           cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCCGGGCGCGATCGGCGCCCCCCCACGCGGCGAGGAACAGCAGGCGCGTGGCCTGCGCGCAGCCGa                                                           >  8:226363/1‑134 (MQ=255)
                                                              ggCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCCCGGCGCGCTCGGCGCCCCCCCACACGGCGAGGCAAAGCACGCGCTTGGCCAGCGCCCAGCCgcgccg                                                       >  6:63888/1‑130 (MQ=255)
                                                                  cgtgcggcggCAACGGCAGCCAACGGCGCGGCGGCGCAGACTGCGCTGACCAACCCGGCGG‑CCTGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  6:130369/131‑1 (MQ=255)
                                                                  actgcggcggCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                   <  4:111858/131‑1 (MQ=255)
                                                                             aaCGCCAGGAAACTCCGGGTCGGCGCAGACTTCGCTCCCAAACCCGGGGG‑CCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                        <  7:200070/135‑1 (MQ=255)
                                                                                 gccggcaACTGCTCGTCGGCGCAGACTTCGCTGACCACCCCGGCGG‑CCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCA‑GCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTgg                                   <  2:37703/132‑1 (MQ=255)
                                                                                 gcaggcaACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGTGGCAGGTGCCCGCTg                                    >  8:202906/1‑135 (MQ=255)
                                                                                        aCTGCGCGGCGCCCCAGACTGCGCTCCACACCCCGGCGC‑CCGGGGCGCGCGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCgg                             <  6:1341/135‑1 (MQ=255)
                                                                                          tGCTCGTCGGCGCAGACTGCGCTGCCCAACCCGGCGC‑CCAGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:162485/135‑1 (MQ=255)
                                                                                          tGCGCGGCGGCGCAGACTGCGCCCACCACCCCGGCGC‑CCCGGCCGCGACCGCCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                           <  6:308195/135‑1 (MQ=255)
                                                                                          gggtcggcgGCGCCCACTGCGCTGACCACCCCGGCGG‑CCCGGGGGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTGGGCGTGCTCGCTGCCGATCAGCCGGGGCAGGTGCCCGCTGGCGCCGGCa                           <  2:38065/132‑1 (MQ=255)
                                                                                               gtcgGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCCCAACGCGGCGAGGGACAGCAGGCGCTTGGCCGGCTCGCGGCCGCTCCGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCggg                      >  4:112908/1‑135 (MQ=255)
                                                                                               gggggCGCTGACGTCGCTTCCCACCCGGGGGG‑CCTGGGCGCTAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                      <  3:253841/132‑1 (MQ=255)
                                                                                                    cgcAGACTTCGCTCCCCACCCCGGCGG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  6:67571/135‑1 (MQ=255)
                                                                                                    cgcAGACTTCGCGCACCAACCCGGCGG‑CCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                 <  3:130413/135‑1 (MQ=255)
                                                                                                       aGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCTGCGCGCTGCCGATCAGCCGTGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGCTGTAGc              >  7:317465/1‑135 (MQ=255)
                                                                                                       aGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCCCCACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCCGCCGGGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCGGTAGc              >  4:282886/1‑135 (MQ=255)
                                                                                                       aGACTTCGCTGACCAACCCGGCGG‑CCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGc              >  8:77876/1‑135 (MQ=255)
                                                                                                                 gACCAACCCGGCGG‑CCACGGCGCGATCCGCGCCCCACAACTCGTCGAGGAACAGCACGCGCTTGGCCTGCTCCCAGCCCATCAGCCGTGGCAGGTGCCAGCTGGCCCCGGCATAGGGGCTGTAGCCCAAGCTGgt    >  6:247881/1‑135 (MQ=255)
                                                                                                                   ccACCCGGGGGG‑CCTGGGCGCTTTGGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:109105/135‑1 (MQ=255)
                                                                                                                   ccACCCGGGCGG‑CCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:148947/135‑1 (MQ=255)
                                                                                                                   ccACCCGGGCGG‑CCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:133327/135‑1 (MQ=255)
                                                                                                                   ccACCCCGGCGG‑CCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  7:77876/135‑1 (MQ=255)
                                                                                                                   ccAACCGGGCGG‑CCTGGGCGGGAGGGGCGCCACACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCGGGCGCCGGCATCTGGGCTGTAGCCCATGCTGgtgt  <  4:241428/135‑1 (MQ=255)
                                                                                                                   ccAACCGGGCGG‑CCCGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  2:5839/135‑1 (MQ=255)
                                                                                                                    ccaCCCGGCGGCCCCGGGGGCCTGCGGCCCCCCACACCTCGTCGCGGAACAGCAGGCGCGTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt  <  5:36371/133‑1 (MQ=255)
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CCGTCGCGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGGCGG‑CCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGT  >  KT2440/4245016‑4245265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑